##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	b.chr21.1.fq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4898
Sequences flagged as poor quality	0
Sequence length	55-75
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.548999591670068	31.0	31.0	34.0	30.0	34.0
2	31.702939975500204	31.0	31.0	34.0	30.0	34.0
3	31.24724377296856	31.0	31.0	34.0	27.0	34.0
4	35.185585953450385	37.0	35.0	37.0	32.0	37.0
5	35.071661902817475	37.0	35.0	37.0	32.0	37.0
6	35.14087382605145	37.0	35.0	37.0	32.0	37.0
7	35.163331972233564	37.0	35.0	37.0	32.0	37.0
8	35.22111065741119	37.0	35.0	37.0	32.0	37.0
9	36.73438138015516	39.0	37.0	39.0	32.0	39.0
10	36.70334830543079	39.0	35.0	39.0	32.0	39.0
11	36.75847284605962	39.0	35.0	39.0	32.0	39.0
12	36.629644752960395	39.0	35.0	39.0	32.0	39.0
13	36.599428338097184	39.0	35.0	39.0	32.0	39.0
14	37.72131482237648	40.0	37.0	41.0	32.0	41.0
15	37.67333605553287	40.0	37.0	41.0	32.0	41.0
16	37.61086157615353	40.0	37.0	41.0	32.0	41.0
17	37.45140873826051	40.0	36.0	41.0	31.0	41.0
18	37.43466721110657	40.0	36.0	41.0	31.0	41.0
19	37.466312780726824	40.0	36.0	41.0	31.0	41.0
20	37.53082890975909	40.0	36.0	41.0	32.0	41.0
21	37.10126582278481	39.0	36.0	41.0	30.0	41.0
22	37.159248672927724	39.0	36.0	41.0	31.0	41.0
23	37.062882809309926	39.0	36.0	41.0	31.0	41.0
24	36.974275214373215	39.0	36.0	41.0	31.0	41.0
25	36.88505512454063	39.0	35.0	41.0	30.0	41.0
26	36.399346672111065	39.0	35.0	41.0	29.0	41.0
27	36.39873417721519	39.0	35.0	41.0	30.0	41.0
28	36.2360146998775	39.0	35.0	41.0	29.0	41.0
29	35.97264189465088	39.0	35.0	41.0	27.0	41.0
30	36.09759085340956	39.0	35.0	40.0	27.0	41.0
31	35.730093915884034	39.0	34.0	40.0	27.0	41.0
32	35.67435688035933	38.0	34.0	40.0	26.0	41.0
33	35.69844834626378	38.0	34.0	40.0	27.0	41.0
34	35.61821151490404	38.0	34.0	40.0	27.0	41.0
35	35.438138015516536	38.0	34.0	40.0	26.0	41.0
36	35.32931808901593	38.0	34.0	40.0	25.0	41.0
37	35.03572886892609	38.0	34.0	40.0	24.0	41.0
38	34.91343405471621	38.0	33.0	40.0	24.0	41.0
39	34.83217639853001	38.0	33.0	40.0	24.0	41.0
40	34.450796243364636	38.0	33.0	40.0	23.0	41.0
41	34.43997550020416	38.0	33.0	40.0	23.0	41.0
42	34.411188240098	38.0	33.0	40.0	23.0	41.0
43	34.13883217639853	38.0	33.0	40.0	22.0	41.0
44	33.949162923642305	38.0	33.0	40.0	21.0	41.0
45	34.00837076357697	38.0	33.0	40.0	22.0	41.0
46	33.88015516537362	38.0	32.0	40.0	22.0	41.0
47	33.55553287055941	38.0	32.0	40.0	20.0	41.0
48	33.55737035524704	37.0	32.0	40.0	20.0	41.0
49	33.360351163740305	37.0	32.0	40.0	20.0	41.0
50	33.50592078399347	37.0	32.0	40.0	20.0	41.0
51	33.47815434871376	37.0	31.0	40.0	21.0	41.0
52	33.58309514087382	37.0	32.0	40.0	21.0	41.0
53	33.27970600244998	37.0	32.0	40.0	20.0	41.0
54	33.22662311147407	37.0	32.0	40.0	20.0	41.0
55	33.370355247039605	37.0	32.0	40.0	21.0	41.0
56	34.40040604556734	38.0	33.0	40.0	25.0	41.0
57	34.309722535528984	38.0	33.0	40.0	25.0	41.0
58	34.18542747575005	37.0	33.0	40.0	23.0	41.0
59	33.99819535303406	37.0	33.0	40.0	24.0	41.0
60	33.883600270697045	37.0	33.0	40.0	24.0	41.0
61	33.69614256711031	37.0	33.0	40.0	23.0	41.0
62	33.47823144597338	36.0	32.0	40.0	22.0	41.0
63	33.251297090006766	36.0	32.0	40.0	22.0	41.0
64	33.06586961425671	36.0	32.0	40.0	21.0	41.0
65	32.90525603428829	36.0	32.0	39.0	21.0	41.0
66	32.8287841191067	36.0	32.0	39.0	21.0	41.0
67	32.7351680577487	35.0	31.0	39.0	22.0	41.0
68	32.63839386420032	35.0	32.0	39.0	21.0	41.0
69	32.35235732009926	35.0	32.0	39.0	19.0	41.0
70	32.17031355741033	35.0	31.0	39.0	18.0	40.0
71	31.910669975186103	35.0	31.0	38.0	17.0	40.0
72	31.492217459959395	35.0	31.0	38.0	7.0	40.0
73	31.237536656891496	35.0	31.0	37.0	2.0	40.0
74	31.024137153169413	35.0	31.0	37.0	2.0	40.0
75	30.47078727723889	34.0	30.0	37.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.01052541208791169
1101	2	-0.1274169117919044
1101	3	0.5369792244792251
1101	4	-0.12179706867206619
1101	5	0.31606764184889613
1101	6	-0.9638438529063578
1101	7	-0.4000119093869188
1101	8	-0.5355360495985622
1101	9	-2.697867815055318
1101	10	-2.0827937109187076
1101	11	-0.7519589207089226
1101	12	-0.32137318699818707
1101	13	-1.9519542089854696
1101	14	0.3727599657287186
1101	15	1.3603727002164447
1101	16	-0.7765683679746118
1101	17	-0.6020051418488848
1101	18	1.1643039830539834
1101	19	-0.5852170109982637
1101	20	-0.7640241124616125
1101	21	1.4934267988955412
1101	22	0.10486469548970234
1101	23	1.1333491450678892
1101	24	1.573524161024153
1101	25	2.591458541458543
1101	26	2.31748711358086
1101	27	1.3924990807803397
1101	28	1.2574536285473883
1101	29	2.7812077101139536
1101	30	1.8338945487383
1101	31	3.0955877166814645
1101	32	2.0909215206090224
1101	33	2.791178902116407
1101	34	2.454208840927585
1101	35	2.431693306693319
1101	36	1.9922850297850374
1101	37	-0.012721277565027833
1101	38	1.1109966422466542
1101	39	2.8694040912790797
1101	40	0.4542728972416441
1101	41	-4.210497112840862
1101	42	0.286036735255486
1101	43	-1.4801400856088236
1101	44	4.371917174260915
1101	45	0.07204835095460282
1101	46	-1.0006784881784867
1101	47	0.17179044913419972
1101	48	-0.06434673544049474
1101	49	2.4222436995874403
1101	50	2.427507677507677
1101	51	5.844807189338454
1101	52	0.8426315582565564
1101	53	2.8568669178044317
1101	54	0.501968633218631
1101	55	2.564099846912356
1101	56	1.3949876036273068
1101	57	3.286986845534635
1101	58	0.3529023298324745
1101	59	1.8727606067679687
1101	60	3.2565987355509236
1101	61	3.8371137134923927
1101	62	3.9649062912849757
1101	63	2.0250070803379643
1101	64	3.0069382959088813
1101	65	3.7247649375406766
1101	66	2.5133079270946794
1101	67	3.750905327651658
1101	68	3.245264318334172
1101	69	1.6170965608465622
1101	70	2.0791849286334596
1101	71	1.2122804449091191
1101	72	1.9322086172269977
1101	73	2.494290676643619
1101	74	2.459877479546602
1101	75	2.323416357607517
1102	1	1.775188873626373
1102	2	1.140440231065238
1102	3	1.786979224479225
1102	4	1.735345788470788
1102	5	1.8874962132774655
1102	6	1.8932990042364963
1102	7	0.8678452334702271
1102	8	1.3573210932585837
1102	9	0.7842750420875362
1102	10	1.4172062890812924
1102	11	1.9623267935767927
1102	12	1.8393410987160976
1102	13	1.8159029338716763
1102	14	2.444188537157288
1102	15	2.217515557359299
1102	16	2.098431632025388
1102	17	1.2551377152939693
1102	18	2.8071611259111293
1102	19	2.3790687032874516
1102	20	2.8431187446812416
1102	21	3.011283941752687
1102	22	1.5512932669182717
1102	23	3.151206287925035
1102	24	3.0020955895955836
1102	25	2.7878871128871126
1102	26	3.835344256438006
1102	27	3.249641937923194
1102	28	3.7038821999759577
1102	29	4.156207710113954
1102	30	3.566037405881154
1102	31	3.5777305738243186
1102	32	4.5730643777518765
1102	33	3.791178902116407
1102	34	3.7577802694990154
1102	35	3.788836163836173
1102	36	4.402999315499322
1102	37	4.433707293863542
1102	38	4.932425213675224
1102	39	4.226546948421934
1102	40	4.614987182955929
1102	41	5.4502171728734226
1102	42	5.661036735255486
1102	43	5.680574200105461
1102	44	3.46120288854663
1102	45	4.679191208097457
1102	46	4.070750083250083
1102	47	4.72536187770563
1102	48	5.024938978845221
1102	49	4.440100842444586
1102	50	5.695364820364823
1102	51	5.576950046481308
1102	52	4.806917272542272
1102	53	5.803295489233001
1102	54	4.805540061790062
1102	55	4.099814132626641
1102	56	3.019987603627307
1102	57	2.911986845534635
1102	58	3.394568996499139
1102	59	3.164427273434633
1102	60	3.3399320688842593
1102	61	3.003780380159057
1102	62	3.38157295795164
1102	63	3.7333404136713
1102	64	3.840271629242217
1102	65	0.058098270874012314
1102	66	1.8466412604280151
1102	67	1.2092386609849939
1102	68	1.0369309850008364
1102	69	3.200429894179898
1102	70	2.5791849286334596
1102	71	3.337280444909119
1102	72	4.057208617226998
1102	73	4.3276240099769545
1102	74	4.876544146213266
1102	75	4.906749690940853
1103	1	0.9180460164835189
1103	2	0.8011545167795227
1103	3	1.4655506530506557
1103	4	1.3067743598993573
1103	5	1.6017819275631808
1103	6	1.7504418613793504
1103	7	1.1714166620416577
1103	8	0.8930353789728684
1103	9	1.1592750420875362
1103	10	1.345777717652723
1103	11	1.3908982221482233
1103	12	1.3214839558589517
1103	13	1.3337600767288222
1103	14	2.2299028228715727
1103	15	1.9318012716450141
1103	16	1.9377173463111035
1103	17	1.6122805724368234
1103	18	1.5214468401968446
1103	19	1.700497274716021
1103	20	1.9502616018240957
1103	21	1.4934267988955412
1103	22	1.6762932669182717
1103	23	1.8476348593536045
1103	24	1.7878098753098683
1103	25	1.5200299700299666
1103	26	2.81748711358086
1103	27	2.606784795066048
1103	28	2.6145964856902424
1103	29	2.9240648529710995
1103	30	2.476751691595439
1103	31	1.4527305738243186
1103	32	1.7337786634661612
1103	33	1.7911789021164068
1103	34	3.5256374123561613
1103	35	3.5745504495504576
1103	36	2.8494278869278915
1103	37	2.487278722434972
1103	38	2.753853785103793
1103	39	3.6551183769933644
1103	40	1.8114157543844982
1103	41	3.0752171728734226
1103	42	2.2146081638269166
1103	43	3.0198599143911764
1103	44	3.1576314599751996
1103	45	3.786334065240311
1103	46	3.285035797535798
1103	47	3.386076163419915
1103	48	4.364224693130936
1103	49	2.350815128158871
1103	50	3.927507677507677
1103	51	5.273378617909877
1103	52	4.8426315582565564
1103	53	3.9997240606615705
1103	54	5.501968633218631
1103	55	4.849814132626641
1103	56	3.933449142088847
1103	57	4.210063768611555
1103	58	5.442645919576059
1103	59	4.462504196511553
1103	60	4.359162838115026
1103	61	4.465318841697517
1103	62	3.8238806502593334
1103	63	4.666032721363607
1103	64	4.801810090780677
1103	65	4.942713655489399
1103	66	5.0389489527357085
1103	67	4.199623276369607
1103	68	4.911930985000836
1103	69	4.911968355718358
1103	70	4.502261851710379
1103	71	4.943049675678353
1103	72	5.393747078765458
1103	73	6.096854779207721
1103	74	5.088082607751726
1103	75	5.925980460171619
1104	1	0.0024616008990996363
1104	2	0.3790765947016048
1104	3	0.900615588115592
1104	4	1.553527606652608
1104	5	1.7056780314592856
1104	6	1.3478444587819496
1104	7	0.7428452334702271
1104	8	0.9709574568949506
1104	9	1.0683659511784427
1104	10	1.1899335618085658
1104	11	0.8714177026676992
1104	12	1.5097956441706444
1104	13	1.5545392975080432
1104	14	0.39873399170274126
1104	15	1.5811519209956657
1104	16	1.8597952683890213
1104	17	1.982410442566696
1104	18	1.7617065804565826
1104	19	-0.4163858421670952
1104	20	0.8203914719539682
1104	21	1.636283941752687
1104	22	0.2217478123728185
1104	23	0.2762062879250351
1104	24	1.274822862322857
1104	25	1.5265234765234794
1104	26	2.0057988018925528
1104	27	0.8860055742868269
1104	28	1.5561549272486843
1104	29	0.5084804373866803
1104	30	1.7819464967902476
1104	31	1.5890942101879517
1104	32	1.7662461959336966
1104	33	1.5184516293891335
1104	34	2.3373257240444687
1104	35	0.10701798201799306
1104	36	1.9143629518629552
1104	37	1.1950709302271747
1104	38	1.6824252136752236
1104	39	-7.257788507928353E-4
1104	40	0.7399871829559288
1104	41	1.0752171728734226
1104	42	-0.713963264744514
1104	43	3.169210563741828
1104	44	1.631657434001177
1104	45	2.0201002990065504
1104	46	4.661659174159176
1104	47	2.4185436958874504
1104	48	3.104484433390674
1104	49	4.0196462969900395
1104	50	1.5703648203648228
1104	51	-0.843504498973239
1104	52	2.011462727087725
1104	53	3.0646591255966342
1104	54	1.8396309708809682
1104	55	2.2134504962630075
1104	56	1.9949876036273082
1104	57	1.8869868455346364
1104	58	2.61956899649914
1104	59	2.539427273434633
1104	60	0.28993206888426215
1104	61	2.003780380159057
1104	62	2.331572957951643
1104	63	2.658340413671297
1104	64	2.54027162924222
1104	65	1.5580982708740123
1104	66	1.046641260428018
1104	67	1.884238660984991
1104	68	1.9119309850008364
1104	69	3.450429894179898
1104	70	2.7791849286334624
1104	71	2.1122804449091177
1104	72	1.9322086172269977
1104	73	-0.9723759900230462
1104	74	-0.07345585378673292
1104	75	0.15674969094085256
1105	1	-0.8157202172827169
1105	2	-0.5300143143893088
1105	3	1.0824337699337718
1105	4	-0.7191996660746653
1105	5	-0.11250378672253447
1105	6	0.4387535496910431
1105	7	-0.25715476652977287
1105	8	0.42550291144040386
1105	9	-0.1134522306397372
1105	10	0.3717517436267457
1105	11	-1.1285822973323008
1105	12	-0.8538407194657225
1105	13	-4.1727334297646905
1105	14	-2.6012660082972587
1105	15	-1.327938988095248
1105	16	-0.9583865497927917
1105	17	-0.7448622847060307
1105	18	-3.965566146816144
1105	19	0.038159612378358077
1105	20	-0.08869943713693829
1105	21	-1.2728069673382194
1105	22	-2.4146158239908218
1105	23	-4.814702802984055
1105	24	-3.1797225922225962
1105	25	-3.564385614385614
1105	26	-3.721473925380174
1105	27	-3.3867216984404394
1105	28	-3.4438450727513157
1105	29	-4.309701380795136
1105	30	-2.945326230482479
1105	31	-4.683633062539318
1105	32	-4.1428447131572135
1105	33	-3.208821097883593
1105	34	-1.4808560941373514
1105	35	-0.7111638361638271
1105	36	-4.812909775409768
1105	37	-3.8958381606819117
1105	38	-5.953938422688413
1105	39	-6.182543960668976
1105	40	-4.805467362498618
1105	41	-5.106601008944761
1105	42	-6.895781446562697
1105	43	-5.739880345349086
1105	44	-3.82288802054428
1105	45	-4.434445155538906
1105	46	-4.156522644022644
1105	47	-5.126910849567096
1105	48	-7.531879202972963
1105	49	-5.525808248464504
1105	50	-5.611453361453361
1105	51	-4.479868135336876
1105	52	-4.079446363821365
1105	53	-5.480795419857909
1105	54	-3.2512781200281218
1105	55	-3.2410949582824493
1105	56	-0.04945684081713608
1105	57	-1.2685687100209222
1105	58	-1.369319892389747
1105	59	-4.016128282120924
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2306	11	-0.43767320642320584
2306	12	-1.8356589012838995
2306	13	-3.8090970661283237
2306	14	-2.055811462842712
2306	15	-2.982484442640704
2306	16	-1.9765683679746147
2306	17	-4.344862284706032
2306	18	-2.8928388740888735
2306	19	-3.8709312967125484
2306	20	-3.9068812553187584
2306	21	-3.16371605824731
2306	22	-4.723706733081727
2306	23	-3.723793712074965
2306	24	-3.5979044104044178
2306	25	-5.237112887112886
2306	26	-3.339655743561991
2306	27	-3.5503580620768034
2306	28	-8.171117800024042
2306	29	-7.818792289886048
2306	30	-4.108962594118843
2306	31	-3.0472694261756814
2306	32	-6.251935622248123
2306	33	-5.008821097883594
2306	34	-3.3172197305009874
2306	35	-2.1111638361638256
2306	36	-0.7220006845006779
2306	37	-2.04129270613646
2306	38	-0.3175747863247764
2306	39	-3.0734530515780634
2306	40	0.9399871829559316
2306	41	-3.724782827126578
2306	42	-4.513963264744515
2306	43	-4.79442579989454
2306	44	-1.9137971114533698
2306	45	-0.6708087919025445
2306	46	-0.22924991674991446
2306	47	2.2003618777056317
2306	48	-4.350061021154779
2306	49	-4.034899157555415
2306	50	-7.6296351796351765
2306	51	-5.698049953518691
2306	52	-7.6430827274577275
2306	53	-4.771704510766998
2306	54	-6.469459938209937
2306	55	-1.1501858673733594
2306	56	4.144987603627307
2306	57	2.536986845534635
2306	58	0.7695689964991388
2306	59	-1.960572726565367
2306	60	0.8399320688842593
2306	61	1.003780380159057
2306	62	-0.11842704204835997
2306	63	-3.8916595863287
2306	64	-1.409728370757783
2306	65	1.0580982708740123
2306	66	-1.1533587395719849
2306	67	-1.6657613390150061
2306	68	1.1619309850008364
2306	69	4.200429894179898
2306	70	2.8291849286334596
2306	71	2.212280444909119
2306	72	2.6822086172269977
2306	73	1.0776240099769545
2306	74	0.6265441462132664
2306	75	0.6567496909408526
2307	1	-0.30814445970695914
2307	2	-0.8178931022680942
2307	3	-0.37968744218744277
2307	4	-0.6813208781958764
2307	5	0.7208295466108012
2307	6	-1.523367662430168
2307	7	-2.007154766529773
2307	8	-1.8093455734080806
2307	9	-2.6740582912457995
2307	10	-2.499460377585372
2307	11	-0.871006539756543
2307	12	-1.7023255679505667
2307	13	-1.8924303994616594
2307	14	-1.6391447961760477
2307	15	-1.1158177759740369
2307	16	-3.776568367974612
2307	17	-2.411528951372695
2307	18	-2.9428388740888707
2307	19	-1.7875979633792127
2307	20	-2.490214588652094
2307	21	-2.280382724913977
2307	22	-2.407040066415064
2307	23	-0.6404603787416292
2307	24	-0.831237743737752
2307	25	-0.6704462204462232
2307	26	-0.289655743561994
2307	27	-3.1670247287434705
2307	28	-3.3377844666907066
2307	29	-3.052125623219382
2307	30	1.4410374058811541
2307	31	-0.21393609284234572
2307	32	-2.0519356222481235
2307	33	0.12451223544974255
2307	34	-0.11721973050098455
2307	35	-1.461163836163827
2307	36	-2.472000684500678
2307	37	-0.9412927061364584
2307	38	-0.6509081196581121
2307	39	-0.2734530515780662
2307	40	-2.343346150377407
2307	41	-1.8414494937932417
2307	42	1.3693700685888217
2307	43	-0.4444257998945389
2307	44	0.0028695552132944613
2307	45	1.7625245414307926
2307	46	2.5707500832500827
2307	47	-1.3996381222943697
2307	48	-0.26672768782144374
2307	49	-2.4348991575554138
2307	50	-1.1796351796351772
2307	51	-1.46471662018536
2307	52	-0.026416060791063956
2307	53	0.011628822566336794
2307	54	3.4305400617900617
2307	55	0.5164807992933049
2307	56	2.194987603627304
2307	57	1.8869868455346364
2307	58	0.41956899649913737
2307	59	0.13942727343463446
2307	60	1.9899320688842579
2307	61	1.003780380159057
2307	62	1.5315729579516386
2307	63	-0.34165958632870286
2307	64	0.04027162924221983
2307	65	1.7580982708740152
2307	66	-0.45335873957198203
2307	67	0.5842386609849939
2307	68	-0.2880690149991665
2307	69	1.1504298941799007
2307	70	1.5791849286334596
2307	71	2.0122804449091163
2307	72	1.1322086172270005
2307	73	2.6276240099769517
2307	74	2.026544146213265
2307	75	3.056749690940851
2308	1	0.275188873626373
2308	2	0.26544023106523795
2308	3	-0.5880207755207749
2308	4	0.9853457884707879
2308	5	0.26249621327746553
2308	6	-0.8567009957635037
2308	7	0.11784523347022713
2308	8	0.6073210932585837
2308	9	0.03427504208753618
2308	10	-0.5827937109187076
2308	11	-2.1626732064232073
2308	12	-0.16065890128390237
2308	13	0.6909029338716763
2308	14	-0.05581146284271199
2308	15	-2.282484442640701
2308	16	1.3484316320253882
2308	17	0.25513771529396934
2308	18	-2.8178388740888707
2308	19	-0.37093129671254843
2308	20	-2.0318812553187584
2308	21	0.5112839417526871
2308	22	0.6762932669182717
2308	23	0.7762062879250351
2308	24	1.6270955895955836
2308	25	1.7878871128871126
2308	26	-3.539655743561994
2308	27	-0.2503580620768062
2308	28	1.7038821999759577
2308	29	-0.9687922898860464
2308	30	-0.6839625941188459
2308	31	1.9527305738243186
2308	32	-0.051935622248123536
2308	33	1.6661789021164068
2308	34	0.8827802694990154
2308	35	-0.33616383616382706
2308	36	-0.2220006845006779
2308	37	-2.0662927061364584
2308	38	-0.06757478632477643
2308	39	1.6015469484219338
2308	40	0.1149871829559288
2308	41	1.3252171728734226
2308	42	-1.213963264744514
2308	43	2.180574200105461
2308	44	1.5862028885466302
2308	45	3.054191208097457
2308	46	4.195750083250083
2308	47	1.1003618777056303
2308	48	1.8999389788452206
2308	49	0.6901008424445862
2308	50	1.8203648203648228
2308	51	2.8269500464813078
2308	52	1.1819172725422717
2308	53	1.928295489233001
2308	54	-0.5694599382099383
2308	55	-0.025185867373359372
2308	56	-1.0335838249441238
2308	57	0.14412970267748904
2308	58	1.6624261393562847
2308	59	1.9679987020060636
2308	60	-0.8386393596871713
2308	61	-0.5676481912695124
2308	62	0.5601443865230706
2308	63	-2.4988024434715577
2308	64	-2.8025855136149254
2308	65	1.5580982708740123
2308	66	0.34664126042801513
2308	67	0.7270958038421398
2308	68	1.0547881278579823
2308	69	-1.2638558201058174
2308	70	0.150613500062029
2308	71	-1.716290983662308
2308	72	0.5036371886555671
2308	73	0.3276240099769545
2308	74	2.055115574784697
2308	75	3.728178262369422
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	3.0
10	6.0
11	11.0
12	7.0
13	23.0
14	21.0
15	26.0
16	24.0
17	33.0
18	22.0
19	28.0
20	32.0
21	29.0
22	33.0
23	37.0
24	44.0
25	57.0
26	76.0
27	72.0
28	103.0
29	112.0
30	136.0
31	195.0
32	223.0
33	242.0
34	274.0
35	321.0
36	437.0
37	560.0
38	751.0
39	866.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.98978549540347	11.95097037793667	19.30541368743616	38.7538304392237
2	23.74438546345447	20.947325438954675	30.257247856267867	25.05104124132299
3	28.84850959575337	23.00939158840343	23.642302980808495	24.499795835034707
4	28.13393221723152	31.053491220906494	18.29318089015925	22.519395671702735
5	29.052674561045322	30.27766435279706	20.70232748060433	19.967333605553286
6	23.478971008574927	34.89179256839526	20.661494487545937	20.967741935483872
7	21.171906900775827	18.231931400571664	38.79134340547162	21.80481829318089
8	23.23397305022458	21.90690077582687	25.724785626786446	29.134340547162108
9	22.17231523070641	23.581053491220906	27.92976725193957	26.316864026133114
10	23.540220498162515	37.99510004083299	20.8248264597795	17.63985300122499
11	28.119256687768022	25.750459464978558	17.66387584235246	28.466408004900963
12	24.275214373213558	23.540220498162515	25.398121682319314	26.786443446304613
13	22.96855859534504	27.92976725193957	26.94977541853818	22.151898734177212
14	23.60628956503982	27.38411272207474	26.996120073514398	22.013477639371043
15	24.989791751735403	25.928950592078397	26.684360963658637	22.396896692527562
16	25.602286647611272	24.520212331563904	25.41853817884851	24.458962841976316
17	25.969783585136792	24.82645977950184	25.84728460596162	23.356472029399754
18	25.13270722743977	27.398938342180486	24.377296855859534	23.091057574520214
19	26.092282564311965	26.76602694977542	23.27480604328297	23.866884442629644
20	25.82686810943242	26.643527970600246	24.438546345447122	23.091057574520214
21	25.112290730910576	26.80685994283381	25.35728868926092	22.72356063699469
22	23.928133932217232	27.664352797060026	25.50020416496529	22.907309105757452
23	24.887709269089424	27.3172723560637	24.72437729685586	23.070641077991016
24	25.4797876684361	27.113107390771745	24.908125765618617	22.498979175173538
25	25.88811759902001	25.806451612903224	24.80604328297264	23.499387505104124
26	25.010208248264597	26.071866067782768	24.989791751735403	23.928133932217232
27	25.33687219273173	25.82686810943242	25.030624744793794	23.805634953042055
28	25.541037158023684	26.235198040016332	25.745202123315643	22.478562678644344
29	25.990200081665986	27.398938342180486	24.703960800326662	21.90690077582687
30	26.357697019191505	26.561861984483464	24.601878317680686	22.478562678644344
31	25.214373213556552	26.25561453654553	25.193956717027362	23.33605553287056
32	23.948550428746426	26.561861984483464	25.949367088607595	23.540220498162515
33	24.39771335238873	25.806451612903224	26.704777460187834	23.091057574520214
34	24.15271539403838	25.33687219273173	25.193956717027362	25.31645569620253
35	23.78521845651286	27.276439363005306	25.173540220498165	23.764801959983668
36	23.70355247039608	26.43936300530829	25.745202123315643	24.111882400979994
37	23.560636994691713	24.80604328297264	25.35728868926092	26.276031033074727
38	23.05022458146182	25.541037158023684	26.500612494895876	24.908125765618617
39	22.743977133523888	25.663536137198857	26.43936300530829	25.153123723968967
40	22.846059616169864	27.113107390771745	25.969783585136792	24.071049407921603
41	24.377296855859534	25.867701102490813	24.785626786443444	24.969375255206206
42	25.398121682319314	24.785626786443444	25.70436913025725	24.111882400979994
43	25.010208248264597	26.868109432421395	24.663127807268275	23.458554512045733
44	24.58146182115149	25.296039199673338	24.193548387096776	25.928950592078397
45	27.337688852592894	25.41853817884851	25.153123723968967	22.090649244589628
46	24.377296855859534	26.276031033074727	24.969375255206206	24.377296855859534
47	23.29522253981217	27.970600244997957	24.846876276031033	23.88730093915884
48	22.662311147407106	25.030624744793794	25.41853817884851	26.888525928950592
49	24.377296855859534	26.235198040016332	24.68354430379747	24.703960800326662
50	23.907717435688035	26.378113515720703	25.07145773785218	24.642711310739077
51	21.110657411188242	25.84728460596162	25.602286647611272	27.439771335238873
52	23.499387505104124	26.010616578195183	25.33687219273173	25.153123723968967
53	23.356472029399754	25.78603511637403	24.82645977950184	26.031033074724373
54	23.560636994691713	25.50020416496529	25.173540220498165	25.765618619844833
55	22.64189465087791	25.50020416496529	24.86729277256023	26.990608411596572
56	24.15971125648545	27.27272727272727	25.693661177532146	22.87390029325513
57	24.38529212722761	26.032032483645384	27.182494924430404	22.400180464696593
58	22.30994811639973	27.52086623054365	27.04714640198511	23.12203925107151
59	23.23482968644259	27.04714640198511	26.866681705391382	22.851342206180917
60	22.693435596661406	27.340401533949922	26.14482291901647	23.82133995037221
61	23.05436498984886	26.753891270020304	26.93435596661403	23.257387773516804
62	23.257387773516804	25.919242048274306	28.445747800586513	22.377622377622377
63	23.573200992555833	25.761335438754795	27.76900518836003	22.89645838032935
64	22.73855177080984	26.12226483194225	27.137378750281975	24.001804646965937
65	22.653429602888085	28.068592057761734	25.699458483754512	23.578519855595665
66	23.573200992555833	27.881795623731108	26.550868486352357	21.994134897360702
67	23.731107602075344	27.475750056395214	26.889239792465595	21.90390254906384
68	24.069478908188586	25.874125874125873	25.53575456801263	24.52064064967291
69	21.858786374915407	26.3252876156102	27.52086623054365	24.295059778930746
70	22.58064516129032	27.04714640198511	26.866681705391382	23.50552673133318
71	23.279945860591024	26.731333182946088	26.731333182946088	23.257387773516804
72	22.964132641551995	26.528310399278144	27.430633882246784	23.076923076923077
73	23.16715542521994	27.40807579517257	26.505752312203924	22.919016467403562
74	22.30994811639973	28.874351454996617	28.400631626438077	20.415068802165575
75	22.986690728626215	26.07714865779382	28.51342206180916	22.42273855177081
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	2.0
16	1.0
17	3.0
18	4.5
19	8.5
20	13.0
21	19.0
22	24.0
23	28.0
24	33.0
25	46.5
26	65.5
27	73.5
28	75.0
29	89.5
30	106.5
31	113.0
32	117.5
33	122.5
34	145.0
35	157.5
36	153.0
37	154.5
38	153.5
39	154.5
40	158.0
41	161.0
42	174.5
43	179.0
44	173.0
45	173.0
46	175.5
47	169.5
48	159.5
49	167.0
50	190.0
51	188.5
52	181.5
53	187.0
54	179.0
55	161.5
56	149.0
57	133.5
58	134.5
59	145.0
60	139.0
61	130.0
62	110.5
63	94.5
64	89.0
65	80.5
66	70.0
67	57.5
68	46.5
69	43.0
70	33.0
71	24.0
72	22.5
73	23.0
74	17.5
75	13.5
76	15.0
77	13.5
78	7.5
79	4.0
80	5.0
81	14.5
82	25.0
83	116.0
84	206.0
85	114.0
86	14.5
87	7.0
88	4.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061249489587586775
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.02041649652919559
12	0.0
13	0.0
14	0.02041649652919559
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.022558087074216106
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
55	465.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	4433.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.44589628419763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.21113074204946	90.7921600653328
2	0.8613074204946997	1.5924867292772562
3	0.441696113074205	1.2249897917517354
4	0.11042402826855124	0.4083299305839118
5	0.08833922261484099	0.4083299305839118
6	0.0	0.0
7	0.022084805653710248	0.14291547570436913
8	0.022084805653710248	0.16333197223356472
9	0.0	0.0
>10	0.2208480565371025	4.042466312780727
>50	0.022084805653710248	1.2249897917517354
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGC	60	1.2249897917517354	No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCC	36	0.7349938750510413	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	31	0.6329113924050633	No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC	29	0.5920783993466721	No Hit
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC	20	0.4083299305839118	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAG	16	0.32666394446712943	No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC	15	0.30624744793793385	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT	15	0.30624744793793385	TruSeq Adapter, Index 7 (100% over 55bp)
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	13	0.2654144548795427	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAA	12	0.2449979583503471	No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG	11	0.2245814618211515	No Hit
TGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGAC	8	0.16333197223356472	No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC	7	0.14291547570436913	No Hit
CCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	5	0.10208248264597795	No Hit
AAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTT	5	0.10208248264597795	No Hit
TCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCA	5	0.10208248264597795	No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	5	0.10208248264597795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02041649652919559	0.0	0.0	0.0	0.0
2	0.02041649652919559	0.0	0.0	0.0	0.0
3	0.02041649652919559	0.0	0.0	0.0	0.0
4	0.02041649652919559	0.0	0.0	0.0	0.0
5	0.02041649652919559	0.0	0.0	0.0	0.0
6	0.02041649652919559	0.0	0.0	0.0	0.0
7	0.02041649652919559	0.0	0.0	0.0	0.0
8	0.02041649652919559	0.0	0.0	0.0	0.0
9	0.02041649652919559	0.0	0.0	0.0	0.0
10	0.02041649652919559	0.0	0.0	0.0	0.0
11	0.02041649652919559	0.0	0.0	0.0	0.0
12	0.02041649652919559	0.0	0.0	0.0	0.0
13	0.02041649652919559	0.0	0.0	0.0	0.0
14	0.02041649652919559	0.0	0.0	0.0	0.0
15	0.02041649652919559	0.0	0.0	0.0	0.0
16	0.02041649652919559	0.0	0.0	0.0	0.0
17	0.02041649652919559	0.0	0.0	0.0	0.0
18	0.02041649652919559	0.0	0.0	0.0	0.0
19	0.02041649652919559	0.0	0.0	0.0	0.0
20	0.02041649652919559	0.0	0.0	0.0	0.0
21	0.02041649652919559	0.0	0.0	0.0	0.0
22	0.02041649652919559	0.0	0.0	0.0	0.0
23	0.02041649652919559	0.0	0.0	0.0	0.0
24	0.02041649652919559	0.0	0.0	0.0	0.0
25	0.02041649652919559	0.0	0.0	0.0	0.0
26	0.02041649652919559	0.0	0.0	0.0	0.0
27	0.02041649652919559	0.0	0.0	0.0	0.0
28	0.02041649652919559	0.0	0.0	0.0	0.0
29	0.02041649652919559	0.0	0.0	0.0	0.0
30	0.02041649652919559	0.0	0.0	0.0	0.0
31	0.02041649652919559	0.0	0.0	0.0	0.0
32	0.02041649652919559	0.0	0.0	0.0	0.0
33	0.02041649652919559	0.0	0.0	0.0	0.0
34	0.02041649652919559	0.0	0.0	0.0	0.0
35	0.02041649652919559	0.0	0.0	0.0	0.0
36	0.02041649652919559	0.0	0.0	0.0	0.0
37	0.02041649652919559	0.0	0.0	0.0	0.0
38	0.02041649652919559	0.0	0.0	0.0	0.0
39	0.02041649652919559	0.0	0.0	0.0	0.0
40	0.04083299305839118	0.0	0.0	0.0	0.0
41	0.04083299305839118	0.0	0.0	0.0	0.0
42	0.04083299305839118	0.0	0.0	0.0	0.0
43	0.04083299305839118	0.0	0.0	0.0	0.0
44	0.04083299305839118	0.0	0.0	0.0	0.0
45	0.04083299305839118	0.0	0.0	0.0	0.0
46	0.04083299305839118	0.0	0.0	0.0	0.0
47	0.04083299305839118	0.0	0.0	0.0	0.0
48	0.04083299305839118	0.0	0.0	0.0	0.0
49	0.06124948958758677	0.0	0.0	0.0	0.0
50	0.06124948958758677	0.0	0.0	0.0	0.0
51	0.06124948958758677	0.0	0.0	0.0	0.0
52	0.06124948958758677	0.0	0.0	0.0	0.0
53	0.06124948958758677	0.0	0.0	0.0	0.0
54	0.06124948958758677	0.0	0.0	0.0	0.0
55	0.06124948958758677	0.0	0.0	0.0	0.0
56	0.06124948958758677	0.0	0.0	0.0	0.0
57	0.06124948958758677	0.0	0.0	0.0	0.0
58	0.06124948958758677	0.0	0.0	0.0	0.0
59	0.06124948958758677	0.0	0.0	0.0	0.0
60	0.06124948958758677	0.0	0.0	0.0	0.0
61	0.06124948958758677	0.0	0.0	0.0	0.0
62	0.06124948958758677	0.0	0.0	0.0	0.0
63	0.06124948958758677	0.0	0.0	0.0	0.0
64	0.06124948958758677	0.0	0.0	0.0	0.0
>>END_MODULE
