##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	b.chr21.2.fq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4898
Sequences flagged as poor quality	0
Sequence length	50-75
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.698856676194364	31.0	30.0	34.0	25.0	34.0
2	29.925479787668436	31.0	30.0	34.0	25.0	34.0
3	29.950592078399346	31.0	30.0	34.0	25.0	34.0
4	33.34279297672519	37.0	33.0	37.0	26.0	37.0
5	33.33585136790527	37.0	35.0	37.0	28.0	37.0
6	33.11698652511229	37.0	35.0	37.0	25.0	37.0
7	33.060841159657	37.0	33.0	37.0	26.0	37.0
8	33.19130257247856	37.0	35.0	37.0	26.0	37.0
9	34.58472846059616	38.0	35.0	39.0	26.0	39.0
10	34.60861576153532	38.0	35.0	39.0	27.0	39.0
11	34.51429154757044	38.0	35.0	39.0	26.0	39.0
12	34.43772968558596	38.0	35.0	39.0	25.0	39.0
13	34.19436504695794	38.0	34.0	39.0	25.0	39.0
14	35.335647202939974	39.0	35.0	41.0	25.0	41.0
15	34.72233564720294	39.0	34.0	41.0	18.0	41.0
16	35.063086974275215	39.0	34.0	41.0	24.0	41.0
17	35.05532870559412	39.0	34.0	41.0	25.0	41.0
18	34.90506329113924	39.0	34.0	41.0	23.0	41.0
19	34.82155982033483	39.0	34.0	41.0	21.0	41.0
20	34.59024091465904	39.0	34.0	41.0	18.0	41.0
21	34.51653736218865	39.0	34.0	41.0	18.0	41.0
22	34.46141282155982	39.0	34.0	41.0	18.0	41.0
23	34.193752552062065	39.0	33.0	41.0	18.0	41.0
24	34.17803184973459	39.0	33.0	41.0	18.0	41.0
25	34.008983258472846	39.0	33.0	41.0	17.0	41.0
26	33.98182931808902	38.0	33.0	41.0	16.0	41.0
27	34.017149857084526	38.0	33.0	41.0	16.0	41.0
28	33.69211923233973	38.0	33.0	41.0	15.0	41.0
29	33.60534912209065	38.0	33.0	41.0	15.0	41.0
30	33.420375663536134	38.0	33.0	40.0	11.0	41.0
31	33.36749693752552	38.0	33.0	40.0	15.0	41.0
32	32.984075132707225	38.0	32.0	40.0	9.0	41.0
33	33.16210698244181	38.0	33.0	40.0	10.0	41.0
34	33.19967333605553	38.0	32.0	40.0	10.0	41.0
35	33.11514904042466	38.0	32.0	40.0	9.0	41.0
36	33.095140873826054	38.0	32.0	40.0	9.0	41.0
37	28.325438954675377	36.0	16.0	40.0	2.0	41.0
38	29.78828093099224	36.0	17.0	40.0	8.0	41.0
39	31.189465087790936	36.0	28.0	40.0	7.0	41.0
40	31.714985708452428	37.0	30.0	40.0	7.0	41.0
41	31.919763168640262	37.0	30.0	40.0	6.0	41.0
42	32.066149448754594	38.0	30.0	40.0	2.0	41.0
43	31.988566761943652	38.0	30.0	40.0	2.0	41.0
44	31.86729277256023	38.0	30.0	40.0	2.0	41.0
45	31.917109024091467	38.0	30.0	40.0	2.0	41.0
46	31.748060432829725	37.0	30.0	40.0	2.0	41.0
47	31.442017149857083	37.0	30.0	40.0	2.0	41.0
48	31.492241731318906	37.0	30.0	40.0	2.0	41.0
49	31.625969783585138	37.0	30.0	40.0	2.0	41.0
50	31.401184156798692	37.0	30.0	40.0	2.0	41.0
51	30.389351081530783	35.0	28.0	38.0	2.0	40.0
52	30.692179700499167	36.0	29.0	39.0	2.0	40.0
53	31.318843594009984	37.0	30.0	39.0	2.0	41.0
54	31.40016638935108	37.0	30.0	40.0	2.0	41.0
55	31.527870216306155	37.0	30.0	40.0	2.0	41.0
56	34.94491106719368	38.0	34.0	40.0	26.0	41.0
57	34.79866600790514	38.0	34.0	40.0	26.0	41.0
58	34.67045454545455	38.0	33.0	40.0	26.0	41.0
59	34.546689723320156	38.0	33.0	40.0	26.0	41.0
60	34.31595849802372	37.0	33.0	40.0	26.0	41.0
61	34.11338932806324	37.0	33.0	40.0	25.0	41.0
62	34.018033596837945	37.0	33.0	40.0	25.0	41.0
63	33.956768774703555	37.0	33.0	40.0	24.0	41.0
64	33.73344861660079	36.0	33.0	40.0	24.0	41.0
65	33.701581027667984	36.0	33.0	39.0	24.0	41.0
66	33.53557312252964	36.0	33.0	39.0	25.0	41.0
67	33.18997035573123	36.0	32.0	39.0	23.0	41.0
68	32.93453557312253	35.0	32.0	39.0	22.0	41.0
69	32.928112648221344	35.0	32.0	39.0	22.0	41.0
70	32.879199604743086	35.0	32.0	39.0	23.0	41.0
71	32.66057312252964	35.0	32.0	39.0	21.0	41.0
72	32.51679841897233	35.0	32.0	39.0	20.0	40.0
73	32.27470355731225	35.0	32.0	38.0	20.0	40.0
74	32.09659090909091	35.0	32.0	38.0	20.0	40.0
75	31.538290513833992	35.0	31.0	37.0	18.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.9540251126188686
1101	2	1.5082780645280742
1101	3	1.6580119533244542
1101	4	-0.6284586651774191
1101	5	0.3554763523513529
1101	6	1.9511581300643854
1101	7	2.274980112480108
1101	8	1.904690564065561
1101	9	0.3203278492341042
1101	10	1.7564080017205086
1101	11	1.9485004578754541
1101	12	0.37881059912310633
1101	13	3.082514360639358
1101	14	2.4642781408406265
1101	15	2.8997995349557826
1101	16	2.306636274605026
1101	17	2.500932487651255
1101	18	3.937370673308166
1101	19	5.374418145511896
1101	20	5.170339816433561
1101	21	4.9560806092056
1101	22	2.6756048927924
1101	23	4.627479407166895
1101	24	3.9202171670921544
1101	25	1.227919100575356
1101	26	3.6141611282236354
1101	27	2.8510380822880776
1101	28	3.473353324915834
1101	29	1.5915626040626094
1101	30	0.4801534403096852
1101	31	2.3644502199189716
1101	32	3.5334479756354753
1101	33	2.8093886148573617
1101	34	3.0349793146668134
1101	35	4.493390608234357
1101	36	2.5737267651330242
1101	37	5.482431717587961
1101	38	4.099494573713329
1101	39	3.7222363284863285
1101	40	3.141924192705442
1101	41	1.881105757668255
1101	42	2.573333408489649
1101	43	-0.004919993201255579
1101	44	0.12325313575313501
1101	45	0.37081520909645604
1101	46	-1.863908574064812
1101	47	0.2798479876604887
1101	48	-0.6664127250064773
1101	49	-2.1937349398286905
1101	50	-1.9781161835849375
1101	51	-2.960565360565365
1101	52	-2.5766274524087045
1101	53	-3.954703138296882
1101	54	-3.3591402232027257
1101	55	-1.2298951916139416
1101	56	0.727262552262566
1101	57	0.17103642884892167
1101	58	-0.24903042559292032
1101	59	1.644337433399933
1101	60	1.546576918451919
1101	61	3.688317932067932
1101	62	4.629425320050309
1101	63	1.0461529789654733
1101	64	1.8472559269434328
1101	65	3.0792458698708742
1101	66	0.9775449087949042
1101	67	1.639571077071075
1101	68	2.856252601565103
1101	69	4.877802694990187
1101	70	3.53580112017611
1101	71	2.630987357549863
1101	72	4.878722434972438
1101	73	5.265546028046025
1101	74	5.667375506438017
1101	75	3.175859614922114
1102	1	2.3825965411902956
1102	2	0.8475637788137895
1102	3	0.6222976676101695
1102	4	0.4608270491082962
1102	5	0.1590477809227835
1102	6	1.3083009872072395
1102	7	2.7214086839086775
1102	8	3.261833421208415
1102	9	0.4453278492341042
1102	10	-0.297163426850922
1102	11	0.9127861721611694
1102	12	2.9859534562659604
1102	13	0.9218000749250734
1102	14	3.767849569412057
1102	15	2.3105138206700673
1102	16	3.8959219888907413
1102	17	5.108075344794109
1102	18	1.7587992447367355
1102	19	4.410132431226181
1102	20	4.723911245004992
1102	21	5.688223466348454
1102	22	4.211319178506685
1102	23	2.8417651214526103
1102	24	2.973788595663585
1102	25	2.727919100575356
1102	26	4.435589699652205
1102	27	6.190323796573793
1102	28	1.8840676106301188
1102	29	5.1987054612054635
1102	30	6.783724868881116
1102	31	2.953735934204687
1102	32	3.1405908327783294
1102	33	2.9879600434287923
1102	34	2.570693600381098
1102	35	3.225533465377211
1102	36	3.002298193704455
1102	37	8.071717431873676
1102	38	5.545923145141899
1102	39	4.668664899914898
1102	40	4.338352764134012
1102	41	4.184677186239686
1102	42	3.6269048370610797
1102	43	4.012937149655887
1102	44	3.623253135753135
1102	45	3.942243780525029
1102	46	4.77894856879233
1102	47	4.404847987660489
1102	48	4.19073013213638
1102	49	3.4312650601713095
1102	50	4.682598102129347
1102	51	3.8965774965774926
1102	52	4.316229690448438
1102	53	3.670296861703118
1102	54	4.176574062511559
1102	55	4.430819094100343
1102	56	4.527262552262563
1102	57	3.999607857420351
1102	58	3.6938267172642227
1102	59	5.530051719114219
1102	60	6.289434061309066
1102	61	5.288317932067933
1102	62	5.572282462907452
1102	63	5.074724407536905
1102	64	5.1615416412291495
1102	65	4.907817298442303
1102	66	5.034687765937761
1102	67	3.8681425056425027
1102	68	5.341966887279391
1102	69	5.163516980704472
1102	70	4.164372548747544
1102	71	5.37384450040701
1102	72	5.135865292115291
1102	73	3.6655460280460233
1102	74	5.153089792152304
1102	75	5.375859614922117
1103	1	-0.6174034588097044
1103	2	-0.7774362211862105
1103	3	-0.8419880466755458
1103	4	-0.2713158080345579
1103	5	0.2126192094942141
1103	6	0.6654438443500936
1103	7	-0.9393056018056072
1103	8	-0.3095951502201544
1103	9	0.7488992778055348
1103	10	-1.172163426850922
1103	11	0.09135760073260002
1103	12	0.16452488483739103
1103	13	0.29680007492507343
1103	14	-0.10715043058794294
1103	15	0.3283709635272132
1103	16	0.8780648460335954
1103	17	0.21521820193696328
1103	18	1.6516563875938814
1103	19	0.4458467169404656
1103	20	2.4560541021478457
1103	21	-0.6153479622229767
1103	22	0.3184620356495387
1103	23	-1.0868063071188203
1103	24	-1.4369256900506997
1103	25	-0.05779518513892867
1103	26	-0.028696014633510458
1103	27	0.06532379657379295
1103	28	0.11621046777297295
1103	29	-0.3370088245088212
1103	30	-0.8055608454045995
1103	31	0.15016450563325634
1103	32	0.1048765470640447
1103	33	-1.40489709942835
1103	34	-1.107877828190329
1103	35	0.7076763225200651
1103	36	0.9308696222758854
1103	37	5.625288860445107
1103	38	2.4566374308561834
1103	39	2.507950614200613
1103	40	2.284781335562581
1103	41	0.09539147195397035
1103	42	0.07333340848964909
1103	43	-1.0763485646298285
1103	44	-1.6624611499611497
1103	45	-1.8434705051892557
1103	46	-1.435337145493385
1103	47	-2.577294869482369
1103	48	-1.0949841535779044
1103	49	-0.8365920826858328
1103	50	0.7361695307007743
1103	51	-0.460565360565365
1103	52	-0.5766274524087045
1103	53	-0.1689888525825971
1103	54	-1.8591402232027257
1103	55	-1.3013237630425145
1103	56	-0.05607078107077257
1103	57	0.48770309551558455
1103	58	0.7176362410737411
1103	59	0.1610041000665987
1103	60	0.47991025178525604
1103	61	0.12165126540126892
1103	62	-1.4039080132830293
1103	63	-1.6871803543678574
1103	64	-0.3860774063899015
1103	65	-2.6040874634624593
1103	66	-0.572455091205093
1103	67	0.18957107707107212
1103	68	1.4729192682317702
1103	69	0.7944693616568514
1103	70	1.5691344535094487
1103	71	1.8976540242165285
1103	72	1.6953891016391012
1103	73	2.0822126947126876
1103	74	2.034042173104684
1103	75	0.7925262815887848
1104	1	-0.7992216406278878
1104	2	0.6771092333592428
1104	3	1.7359340312465328
1104	4	0.44946341274466306
1104	5	2.9658659627409634
1104	6	-0.6462444673382137
1104	7	0.48277232027231065
1104	8	0.03456069393568839
1104	9	0.5021460310522841
1104	10	0.6005638458763514
1104	11	0.9355134448884428
1104	12	2.145044365356867
1104	13	2.9786182567432533
1104	14	2.074667751230237
1104	15	2.094604729760974
1104	16	1.3845583525271081
1104	17	1.1762571629759293
1104	18	3.236071972009462
1104	19	1.0237687948625478
1104	20	1.871638517732265
1104	21	1.2677689208939071
1104	22	2.9613191785066846
1104	23	2.6144923941798837
1104	24	1.6215158683908584
1104	25	0.8642827369389892
1104	26	0.6401351541976581
1104	27	1.2925965238465196
1104	28	1.9408857924482987
1104	29	1.1759781884781901
1104	30	0.9087248688811158
1104	31	1.896917752386507
1104	32	1.5724090145965093
1104	33	-1.4893126838439343
1104	34	0.7752390549265513
1104	35	1.5323516471953909
1104	36	1.820480011886275
1104	37	6.1058083409645825
1104	38	5.216377690596445
1104	39	3.5663921726421712
1104	40	3.2588073095885584
1104	41	2.1278590044215058
1104	42	1.7291775643338063
1104	43	2.2288462405649803
1104	44	2.486889499389502
1104	45	2.2149710532523024
1104	46	2.369857659701424
1104	47	2.4616661694786686
1104	48	0.3725483139545638
1104	49	1.6244468783531296
1104	50	2.9439617384929804
1104	51	1.7147593147593092
1104	52	1.9298660540848047
1104	53	2.408933225339485
1104	54	0.4720286079661058
1104	55	1.3285463668276165
1104	56	3.527262552262563
1104	57	2.904369762182256
1104	58	3.4398584632959626
1104	59	3.7998929889554915
1104	60	3.7021324740074775
1104	61	3.066095709845712
1104	62	3.651647542272528
1104	63	2.6461529789654747
1104	64	3.0028114824989913
1104	65	3.3681347587597585
1104	66	4.844211575461571
1104	67	5.106237743737736
1104	68	5.500697046009549
1104	69	1.7666915838790729
1104	70	4.5135788979538916
1104	71	4.230987357549864
1104	72	4.500944657194658
1104	73	5.109990472490466
1104	74	5.422931061993577
1104	75	3.7091929482554526
1105	1	1.655323813917569
1105	2	0.8589274151774262
1105	3	-0.6277023323898305
1105	4	-1.5505365872553405
1105	5	1.874956871831877
1105	6	2.4446646235708727
1105	7	1.3009541384541308
1105	8	1.761833421208415
1105	9	1.502146031052284
1105	10	1.4187456640581715
1105	11	3.1173316267066227
1105	12	1.963226183538687
1105	13	3.069527347652347
1105	14	1.256485933048424
1105	15	3.1855138206700673
1105	16	1.7481947161634679
1105	17	0.721711708430476
1105	18	3.236071972009462
1105	19	-1.7944130233192723
1105	20	-0.8556342095404617
1105	21	-0.2776856245606396
1105	22	0.6885919057794112
1105	23	0.4326742123617038
1105	24	-0.10575685888186825
1105	25	0.13701000966626253
1105	26	1.4583169723794782
1105	27	1.8380510693010663
1105	28	2.395431246993752
1105	29	1.8123418248418304
1105	30	1.545088505244749
1105	31	2.260554116022867
1105	32	2.7542271964146963
1105	33	0.6015964070651592
1105	34	-0.40657912689162856
1105	35	2.0778061926499376
1105	36	-0.8158836244773653
1105	37	6.196717431873676
1105	38	4.216377690596445
1105	39	4.0209376271876245
1105	40	3.4406254914067382
1105	41	1.4005862771487791
1105	42	0.9109957461519862
1105	43	1.956118967837707
1105	44	2.3959804084804084
1105	45	2.2149710532523024
1105	46	4.551675841519604
1105	47	4.188938896751395
1105	48	3.554366495772747
1105	49	1.5335377874440361
1105	50	3.671234465765714
1105	51	2.2602138602138595
1105	52	1.7480478722666177
1105	53	2.590751407157665
1105	54	4.0174831534206525
1105	55	1.1467281850094366
1105	56	1.4272625522625617
1105	57	1.0710364288489203
1105	58	1.4509695744070754
1105	59	2.0443374333999316
1105	60	2.1465769184519203
1105	61	-0.8116820679320682
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2306	20	4.198911245004993
2306	21	4.413223466348455
2306	22	2.7613191785066817
2306	23	4.541765121452613
2306	24	1.7487885956635836
2306	25	2.6279191005753546
2306	26	4.585589699652203
2306	27	1.565323796573793
2306	28	1.559067610630116
2306	29	3.2487054612054607
2306	30	2.3087248688811144
2306	31	4.4787359342046855
2306	32	1.5905908327783322
2306	33	1.8379600434287937
2306	34	2.7206936003810966
2306	35	2.550533465377214
2306	36	-0.5977018062955466
2306	37	6.5967174318736745
2306	38	6.470923145141896
2306	39	3.293664899914898
2306	40	2.5133527641340088
2306	41	3.109677186239683
2306	42	4.00190483706108
2306	43	3.5379371496558853
2306	44	-9.076746864246864
2306	45	-0.2577562194749703
2306	46	-1.1210514312076683
2306	47	-1.520152012339512
2306	48	-0.009269867863618941
2306	49	-0.9937349398286912
2306	50	0.10759810212934795
2306	51	4.0965774965774955
2306	52	3.1662296904484393
2306	53	0.6452968617031196
2306	54	3.726574062511556
2306	55	0.055819094100343136
2306	56	-3.672737447737436
2306	57	-2.8289635711510783
2306	58	-0.04903042559292459
2306	59	2.4443374333999373
2306	60	0.3465769184519232
2306	61	0.6883179320679318
2306	62	2.4294253200503064
2306	63	0.8461529789654776
2306	64	2.2472559269434313
2306	65	0.4792458698708728
2306	66	2.5775449087949056
2306	67	1.639571077071075
2306	68	2.6562526015651073
2306	69	-1.7221973050098143
2306	70	0.735801120176113
2306	71	3.630987357549863
2306	72	-0.1212775650275617
2306	73	2.6655460280460233
2306	74	-0.13262449356198047
2306	75	2.175859614922114
2307	1	-1.1174034588097044
2307	2	-0.5274362211862105
2307	3	-1.9610356657231627
2307	4	-0.3308396175583681
2307	5	0.7007144475894478
2307	6	0.9749676538739038
2307	7	-1.0702579827579868
2307	8	-0.988166578791585
2307	9	0.15366118256743988
2307	10	1.7445032398157423
2307	11	-1.4205471611721663
2307	12	-0.5140465437340396
2307	13	0.13013340825840913
2307	14	1.0595162360787214
2307	15	0.3521804873367316
2307	16	2.104255322224077
2307	17	3.0247420114607735
2307	18	-1.8245340885966002
2307	19	-2.089867568773819
2307	20	-2.234422088328344
2307	21	-3.2701098669848783
2307	22	-2.1220141548266476
2307	23	-1.9915682118807219
2307	24	-0.7345447376697507
2307	25	-4.605414232757976
2307	26	-3.064410300347795
2307	27	-0.18467620342620705
2307	28	-1.0742657227032169
2307	29	-0.6346278721278722
2307	30	-2.007941797785552
2307	31	-0.8379307324619774
2307	32	1.5572574994449937
2307	33	-0.178706623237872
2307	34	0.7373602670477624
2307	35	-2.149466534622789
2307	36	-0.41436847296220947
2307	37	2.446717431873676
2307	38	1.5042564784752308
2307	39	-1.37300176675177
2307	40	-1.9533139025326562
2307	41	-1.6069894804269822
2307	42	-1.8314284962722525
2307	43	-1.6120628503441132
2307	44	-0.21008019758019714
2307	45	-2.974422886141639
2307	46	-0.38771809787433753
2307	47	-2.4701520123395113
2307	48	-2.3092698678636197
2307	49	-3.4437349398286905
2307	50	-1.1924018978706528
2307	51	-5.9367558367558395
2307	52	-2.433770309551562
2307	53	-2.038036471630214
2307	54	0.3432407291782269
2307	55	-3.694180905899657
2307	56	-0.8727374477374354
2307	57	-1.1289635711510826
2307	58	-3.749030425592924
2307	59	-4.455662566600065
2307	60	-1.5534230815480825
2307	61	-2.6116820679320654
2307	62	-4.870574679949694
2307	63	-4.753847021034524
2307	64	-1.752744073056565
2307	65	-2.520754130129127
2307	66	-6.022455091205092
2307	67	-2.6604289229289293
2307	68	-2.0437473984348955
2307	69	0.1778026949901843
2307	70	0.6358011201761116
2307	71	1.0309873575498614
2307	72	0.27872243497243687
2307	73	-0.33445397195397675
2307	74	-0.8326244935619798
2307	75	1.375859614922117
2308	1	-1.6174034588097044
2308	2	-0.5274362211862105
2308	3	1.8722976676101695
2308	4	-0.2891729508917038
2308	5	1.6590477809227835
2308	6	1.0583009872072395
2308	7	1.2214086839086775
2308	8	-1.113166578791585
2308	9	1.9453278492341042
2308	10	1.077836573149078
2308	11	-0.3372138278388306
2308	12	2.4859534562659604
2308	13	1.4218000749250734
2308	14	2.517849569412057
2308	15	1.4355138206700673
2308	16	2.6459219888907413
2308	17	3.358075344794109
2308	18	4.0087992447367355
2308	19	4.910132431226181
2308	20	5.223911245004992
2308	21	5.313223466348454
2308	22	5.586319178506685
2308	23	4.71676512145261
2308	24	4.473788595663585
2308	25	5.352919100575356
2308	26	4.810589699652205
2308	27	5.440323796573793
2308	28	4.009067610630119
2308	29	5.1987054612054635
2308	30	5.033724868881116
2308	31	2.578735934204687
2308	32	-0.9844091672216706
2308	33	2.6129600434287923
2308	34	2.570693600381098
2308	35	0.725533465377211
2308	36	3.377298193704455
2308	37	5.946717431873676
2308	38	7.170923145141899
2308	39	6.168664899914898
2308	40	3.0883527641340116
2308	41	4.684677186239686
2308	42	4.62690483706108
2308	43	4.512937149655887
2308	44	3.498253135753135
2308	45	4.442243780525029
2308	46	2.5289485687923303
2308	47	5.404847987660489
2308	48	3.6907301321363803
2308	49	4.1812650601713095
2308	50	3.8075981021293472
2308	51	3.7715774965774926
2308	52	-0.43377030955156215
2308	53	4.295296861703118
2308	54	6.551574062511559
2308	55	6.305819094100343
2308	56	-1.5977374477374369
2308	57	2.3210364288489203
2308	58	-0.07403042559292317
2308	59	1.7443374333999344
2308	60	1.6465769184519203
2308	61	2.038317932067933
2308	62	3.1794253200503064
2308	63	2.2711529789654747
2308	64	3.447255926943434
2308	65	3.479245869870873
2308	66	4.052544908794907
2308	67	3.939571077071072
2308	68	3.931252601565106
2308	69	3.377802694990187
2308	70	4.110801120176113
2308	71	2.605987357549864
2308	72	3.278722434972437
2308	73	2.1655460280460233
2308	74	2.4923755064380195
2308	75	3.250859614922117
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111.0
3	14.0
4	25.0
5	19.0
6	18.0
7	17.0
8	26.0
9	30.0
10	31.0
11	23.0
12	31.0
13	20.0
14	35.0
15	33.0
16	26.0
17	23.0
18	37.0
19	33.0
20	39.0
21	37.0
22	44.0
23	37.0
24	53.0
25	51.0
26	70.0
27	78.0
28	84.0
29	115.0
30	121.0
31	143.0
32	203.0
33	216.0
34	257.0
35	337.0
36	421.0
37	612.0
38	713.0
39	638.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.296871805356776	11.981189940707422	18.278470660396646	41.443467593539154
2	24.80604328297264	21.110657411188242	29.39975500204165	24.68354430379747
3	29.603919967333603	22.6827276439363	22.478562678644344	25.23478971008575
4	29.236423029808083	28.603511637403024	17.701102490812577	24.458962841976316
5	29.66516945692119	31.604736627194775	19.35483870967742	19.375255206206614
6	23.05022458146182	34.48346263781135	20.498162515312373	21.968150265414454
7	22.335647202939978	16.557778685177624	38.668844426296445	22.437729685585953
8	23.866884442629644	21.171906900775827	25.765618619844833	29.195590036749692
9	23.560636994691713	22.396896692527562	27.03144140465496	27.011024908125762
10	24.561045324622295	36.66802776643528	20.70232748060433	18.068599428338096
11	28.64434463046141	25.31645569620253	19.048591261739485	26.990608411596572
12	24.642711310739077	23.05022458146182	26.05144957125357	26.25561453654553
13	24.356880359330336	27.521437321355656	26.010616578195183	22.111065741118825
14	26.316864026133114	25.84728460596162	25.663536137198857	22.17231523070641
15	25.84728460596162	24.520212331563904	24.908125765618617	24.72437729685586
16	26.45977950183748	25.193956717027362	24.62229481420988	23.723968966925277
17	25.663536137198857	26.725193956717025	24.193548387096776	23.417721518987342
18	25.602286647611272	26.153532053899553	24.76521028991425	23.478971008574927
19	26.888525928950592	26.3985300122499	24.071049407921603	22.64189465087791
20	27.63480392156863	25.77614379084967	24.142156862745097	22.4468954248366
21	23.907717435688035	28.215598203348307	25.23478971008575	22.64189465087791
22	26.153532053899553	25.541037158023684	25.07145773785218	23.23397305022458
23	26.684360963658637	26.296447529603924	23.866884442629644	23.1523070641078
24	25.50020416496529	26.316864026133114	25.867701102490813	22.31523070641078
25	26.689810087808862	25.137839493567487	24.280171533591997	23.89217888503165
26	26.418946508779094	27.296855859534503	24.5406288280931	21.743568803593302
27	27.072274397713354	25.41853817884851	24.785626786443444	22.72356063699469
28	26.66394446712944	24.948958758677012	25.867701102490813	22.519395671702735
29	26.357697019191505	26.643527970600246	24.31604736627195	22.6827276439363
30	27.603103307472438	26.05144957125357	23.642302980808495	22.703144140465493
31	26.153532053899553	25.9085340955492	25.990200081665986	21.947733768885257
32	25.493455225431124	25.5973405360482	25.784334095158947	23.12487014336173
33	24.86729277256023	24.948958758677012	26.418946508779094	23.764801959983668
34	23.683135973866882	27.603103307472438	24.948958758677012	23.764801959983668
35	26.378113515720703	26.316864026133114	24.15271539403838	23.1523070641078
36	22.743977133523888	26.357697019191505	24.785626786443444	26.11269906084116
37	25.765306122448976	24.072356215213357	25.115955473098328	25.04638218923933
38	23.37688852592895	25.602286647611272	26.704777460187834	24.31604736627195
39	22.988975091874234	25.50020416496529	26.153532053899553	25.35728868926092
40	24.356880359330336	24.663127807268275	27.848101265822784	23.1318905675786
41	23.683135973866882	25.4797876684361	25.928950592078397	24.908125765618617
42	25.622703144140463	26.316864026133114	24.29563086974275	23.764801959983668
43	25.561453654552878	25.806451612903224	24.948958758677012	23.683135973866882
44	26.868109432421395	24.030216414863208	25.010208248264597	24.091465904450796
45	26.19436504695794	24.17313189056758	25.68395263372805	23.948550428746426
46	23.98938342180482	26.94977541853818	24.887709269089424	24.17313189056758
47	23.29522253981217	25.35728868926092	26.031033074724373	25.31645569620253
48	23.66271947733769	25.296039199673338	24.846876276031033	26.19436504695794
49	24.050632911392405	26.276031033074727	25.377705185790116	24.29563086974275
50	25.07145773785218	25.663536137198857	24.561045324622295	24.703960800326662
51	23.148918469217968	26.164725457570714	25.81114808652246	24.875207986688853
52	23.752079866888522	26.22712146422629	24.126455906821963	25.89434276206323
53	23.93926788685524	25.9567387687188	24.958402662229616	25.14559068219634
54	23.356905158069882	25.831946755407653	24.604825291181363	26.206322795341098
55	22.254575707154743	25.915141430948417	25.14559068219634	26.684692179700498
56	23.690711462450594	27.124505928853754	26.457509881422926	22.727272727272727
57	23.789525691699602	26.6304347826087	28.483201581027668	21.09683794466403
58	21.640316205533598	27.643280632411066	27.223320158102766	23.49308300395257
59	21.936758893280633	27.766798418972332	27.445652173913043	22.850790513833992
60	21.813241106719367	28.26086956521739	27.27272727272727	22.65316205533597
61	23.517786561264824	27.84090909090909	27.420948616600793	21.220355731225297
62	22.134387351778656	28.08794466403162	28.063241106719367	21.714426877470355
63	23.097826086956523	26.679841897233203	27.198616600790515	23.023715415019762
64	23.344861660079054	26.82806324110672	27.445652173913043	22.381422924901187
65	23.24604743083004	28.43379446640316	25.691699604743086	22.628458498023715
66	22.628458498023715	29.150197628458496	26.259881422924902	21.961462450592887
67	22.835230084116773	29.985155863433942	25.779317169717963	21.400296882731322
68	22.439386442355268	27.585353785254824	26.07619990103909	23.89905987135082
69	22.56308758040574	26.81840672934191	26.447303315190503	24.171202375061853
70	21.17094861660079	26.679841897233203	28.68083003952569	23.468379446640316
71	21.86264822134387	28.85375494071146	26.729249011857707	22.554347826086957
72	22.18379446640316	29.6195652173913	26.358695652173914	21.83794466403162
73	22.504940711462453	28.458498023715418	26.976284584980238	22.0602766798419
74	21.541501976284586	29.841897233201582	26.877470355731226	21.73913043478261
75	21.615612648221344	29.17490118577075	27.149209486166008	22.0602766798419
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	1.0
13	0.0
14	1.5
15	1.5
16	0.0
17	2.0
18	7.0
19	8.5
20	7.0
21	11.0
22	22.0
23	35.5
24	42.0
25	39.5
26	49.0
27	69.5
28	79.0
29	89.0
30	99.0
31	115.5
32	135.0
33	140.0
34	136.0
35	139.5
36	148.0
37	161.5
38	179.5
39	172.0
40	160.0
41	165.0
42	176.5
43	172.5
44	162.0
45	152.0
46	167.5
47	175.0
48	154.5
49	177.5
50	205.5
51	189.5
52	171.0
53	166.5
54	163.0
55	146.5
56	129.0
57	140.0
58	160.0
59	164.0
60	159.0
61	142.5
62	114.0
63	103.0
64	104.0
65	95.0
66	80.0
67	69.0
68	61.0
69	51.5
70	45.0
71	44.0
72	42.0
73	37.5
74	32.0
75	26.5
76	23.0
77	20.0
78	18.5
79	17.0
80	14.0
81	22.5
82	32.5
83	101.0
84	168.0
85	97.5
86	14.5
87	2.0
88	1.5
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.14291547570436913
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.04083299305839118
21	0.0
22	0.0
23	0.0
24	0.0
25	0.02041649652919559
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.7354022049816251
33	0.0
34	0.0
35	0.0
36	0.0
37	11.964066966108616
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.14822134387351776
68	0.14822134387351776
69	0.14822134387351776
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50	90.0
51	0.0
52	0.0
53	0.0
54	0.0
55	760.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	4048.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.52837893017558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.89848812095032	93.48713760718661
2	0.47516198704103674	0.898325847284606
3	0.1511879049676026	0.4287464271131074
4	0.12958963282937366	0.4899959167006942
5	0.06479481641468683	0.30624744793793385
6	0.0	0.0
7	0.0	0.0
8	0.04319654427645788	0.32666394446712943
9	0.06479481641468683	0.5512454062882809
>10	0.17278617710583152	3.5116374030216413
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGC	42	0.8574928542262148	No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCC	32	0.6533278889342589	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	23	0.46957942017149856	No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC	20	0.4083299305839118	No Hit
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC	19	0.38791343405471623	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAA	14	0.28583095140873827	No Hit
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	11	0.2245814618211515	No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC	11	0.2245814618211515	No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC	9	0.18374846876276033	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAG	9	0.18374846876276033	No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG	9	0.18374846876276033	No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA	8	0.16333197223356472	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTNTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	8	0.16333197223356472	No Hit
GGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCC	5	0.10208248264597795	No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCC	5	0.10208248264597795	No Hit
AGGCAAGGGGCGGGGACGGGCGGTGGCTCGCNTCGCGGCGGACCGCCCGC	5	0.10208248264597795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.02041649652919559	0.0	0.0	0.0	0.0
26	0.02041649652919559	0.0	0.0	0.0	0.0
27	0.02041649652919559	0.0	0.0	0.0	0.0
28	0.02041649652919559	0.0	0.0	0.0	0.0
29	0.02041649652919559	0.0	0.0	0.0	0.0
30	0.02041649652919559	0.0	0.0	0.0	0.0
31	0.02041649652919559	0.0	0.0	0.0	0.0
32	0.02041649652919559	0.0	0.0	0.0	0.0
33	0.02041649652919559	0.0	0.0	0.0	0.0
34	0.04083299305839118	0.0	0.0	0.0	0.0
35	0.04083299305839118	0.0	0.0	0.0	0.0
36	0.04083299305839118	0.0	0.0	0.0	0.0
37	0.04083299305839118	0.0	0.0	0.0	0.0
38	0.04083299305839118	0.0	0.0	0.0	0.0
39	0.04083299305839118	0.0	0.0	0.0	0.0
40	0.06124948958758677	0.0	0.0	0.0	0.0
41	0.06124948958758677	0.0	0.0	0.0	0.0
42	0.06124948958758677	0.0	0.0	0.0	0.0
43	0.06124948958758677	0.0	0.0	0.0	0.0
44	0.06124948958758677	0.0	0.0	0.0	0.0
45	0.06124948958758677	0.0	0.0	0.0	0.0
46	0.06124948958758677	0.0	0.0	0.0	0.0
47	0.06124948958758677	0.0	0.0	0.0	0.0
48	0.06124948958758677	0.0	0.0	0.0	0.0
49	0.06124948958758677	0.0	0.0	0.0	0.0
50	0.06124948958758677	0.0	0.0	0.0	0.0
51	0.06124948958758677	0.0	0.0	0.0	0.0
52	0.06124948958758677	0.0	0.0	0.0	0.0
53	0.06124948958758677	0.0	0.0	0.0	0.0
54	0.06124948958758677	0.0	0.0	0.0	0.0
55	0.06124948958758677	0.0	0.0	0.0	0.0
56	0.06124948958758677	0.0	0.0	0.0	0.0
57	0.06124948958758677	0.0	0.0	0.0	0.0
58	0.06124948958758677	0.0	0.0	0.0	0.0
59	0.06124948958758677	0.0	0.0	0.0	0.0
60	0.06124948958758677	0.0	0.0	0.0	0.0
61	0.06124948958758677	0.0	0.0	0.0	0.0
62	0.06124948958758677	0.0	0.0	0.0	0.0
63	0.06124948958758677	0.0	0.0	0.0	0.0
64	0.06124948958758677	0.0	0.0	0.0	0.0
>>END_MODULE
