##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	A-1-R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4561
Sequences flagged as poor quality	0
Sequence length	20-75
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622451216838414	31.0	31.0	34.0	30.0	34.0
2	31.803332602499452	31.0	31.0	34.0	30.0	34.0
3	31.412190309142733	31.0	31.0	34.0	27.0	34.0
4	35.34049550537163	37.0	35.0	37.0	33.0	37.0
5	35.28677921508441	37.0	35.0	37.0	32.0	37.0
6	35.26288094716071	37.0	35.0	37.0	32.0	37.0
7	35.287436965577726	37.0	35.0	37.0	32.0	37.0
8	35.376014032010524	37.0	35.0	37.0	32.0	37.0
9	36.81911861433896	39.0	37.0	39.0	32.0	39.0
10	36.845647884235916	39.0	37.0	39.0	32.0	39.0
11	36.92523569392677	39.0	37.0	39.0	33.0	39.0
12	36.744354308265734	39.0	37.0	39.0	32.0	39.0
13	36.73514580135935	39.0	37.0	39.0	32.0	39.0
14	37.926551194913394	40.0	37.0	41.0	32.0	41.0
15	37.82591536943653	40.0	37.0	41.0	32.0	41.0
16	37.72001754001315	40.0	37.0	41.0	32.0	41.0
17	37.772418329313744	40.0	37.0	41.0	32.0	41.0
18	37.65972374479281	40.0	36.0	41.0	32.0	41.0
19	37.71629028721772	40.0	37.0	41.0	32.0	41.0
20	37.669151501863624	40.0	37.0	41.0	32.0	41.0
21	37.325213956550364	40.0	36.0	41.0	31.0	41.0
22	37.33809314586995	39.0	36.0	41.0	31.0	41.0
23	37.38277301691936	39.0	36.0	41.0	31.0	41.0
24	37.377997799779976	39.0	36.0	41.0	31.0	41.0
25	37.335610401057735	39.0	36.0	41.0	31.0	41.0
26	36.969274977895665	39.0	36.0	41.0	31.0	41.0
27	37.001769911504425	39.0	35.0	41.0	31.0	41.0
28	36.82015503875969	39.0	35.0	41.0	30.0	41.0
29	36.41916167664671	39.0	35.0	41.0	29.0	41.0
30	36.50655118809682	39.0	35.0	41.0	29.0	41.0
31	36.50311526479751	39.0	35.0	41.0	30.0	41.0
32	36.2644370122631	39.0	35.0	41.0	27.0	41.0
33	36.50591914228278	39.0	35.0	41.0	30.0	41.0
34	36.422276895549096	39.0	35.0	40.0	29.0	41.0
35	36.16128308658591	39.0	35.0	40.0	27.0	41.0
36	36.21613120647046	38.0	35.0	40.0	27.0	41.0
37	36.306306306306304	38.0	35.0	40.0	29.0	41.0
38	36.0124321880651	38.0	34.0	40.0	27.0	41.0
39	35.907176816844014	38.0	34.0	40.0	26.0	41.0
40	35.758784856041714	38.0	34.0	40.0	27.0	41.0
41	35.64477272727273	38.0	34.0	40.0	26.0	41.0
42	35.69533560864619	38.0	34.0	40.0	26.0	41.0
43	35.48928408572731	38.0	33.0	40.0	26.0	41.0
44	35.36643835616438	38.0	34.0	40.0	25.0	41.0
45	35.491083676268865	38.0	33.0	40.0	26.0	41.0
46	35.531407611187525	38.0	33.0	40.0	27.0	41.0
47	35.19398530762167	38.0	33.0	40.0	25.0	41.0
48	35.24873563218391	38.0	33.0	40.0	25.0	41.0
49	35.22457432121491	38.0	33.0	40.0	26.0	41.0
50	35.18692449355433	38.0	33.0	40.0	26.0	41.0
51	35.27264346623646	38.0	33.0	40.0	26.0	41.0
52	35.605451605451606	38.0	33.0	40.0	27.0	41.0
53	35.42711707542804	38.0	33.0	40.0	27.0	41.0
54	35.266821885913856	38.0	33.0	40.0	26.0	41.0
55	35.592	38.0	33.0	40.0	27.0	41.0
56	36.06003039513678	38.0	34.0	40.0	29.0	41.0
57	35.962595419847325	38.0	34.0	40.0	29.0	41.0
58	36.07974522292994	38.0	34.0	40.0	29.0	41.0
59	35.875828658847524	38.0	34.0	40.0	29.0	41.0
60	35.75574272588055	37.0	34.0	40.0	29.0	41.0
61	35.55029434348605	37.0	34.0	40.0	28.0	41.0
62	35.443788501026695	37.0	34.0	40.0	28.0	41.0
63	35.28019572495493	37.0	33.0	40.0	28.0	41.0
64	35.21987096774193	37.0	33.0	40.0	28.0	41.0
65	35.15502070393375	36.0	33.0	40.0	28.0	41.0
66	35.12987012987013	36.0	33.0	39.0	28.0	41.0
67	35.18617992177314	36.0	33.0	39.0	28.0	41.0
68	35.11311861743912	36.0	33.0	39.0	29.0	41.0
69	35.04160084254871	36.0	33.0	39.0	29.0	41.0
70	34.99655446594222	35.0	33.0	39.0	29.0	41.0
71	35.001604278074865	35.0	33.0	39.0	29.0	40.0
72	35.00487144790257	35.0	34.0	39.0	29.0	40.0
73	34.88307692307692	35.0	33.0	38.0	29.0	40.0
74	34.888448643706184	35.0	33.0	38.0	29.0	40.0
75	34.14726324934839	35.0	32.0	37.0	28.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0717379842379877
1101	2	1.6461432999261838
1101	3	1.4980269608558991
1101	4	1.222756611078971
1101	5	0.5833598175703401
1101	6	0.9579785285377369
1101	7	1.2830383188935812
1101	8	0.7400277719356723
1101	9	1.883106133023908
1101	10	1.016586635830059
1101	11	1.0594910054614033
1101	12	1.521012126275295
1101	13	1.4038626334678952
1101	14	0.9500609374787103
1101	15	2.5303589076615367
1101	16	2.176974685280605
1101	17	2.02830320873084
1101	18	1.8436743903026738
1101	19	2.1347724887198467
1101	20	2.4906729783374573
1101	21	2.5277116158695137
1101	22	2.7530807460083864
1101	23	2.589086419185115
1101	24	2.782610822413446
1101	25	2.727286486661484
1101	26	2.9703363187738177
1101	27	3.197834283771776
1101	28	1.2433411495911528
1101	29	3.325995069745069
1101	30	2.3862967472342405
1101	31	1.770658219095722
1101	32	2.559568267380776
1101	33	2.0727025289525187
1101	34	3.1641341875716975
1101	35	3.4382329476079576
1101	36	1.8295069513819655
1101	37	2.24867458929959
1101	38	2.284742745680248
1101	39	2.3929812086062157
1101	40	3.455646892595432
1101	41	2.5695112587575863
1101	42	2.582505080023452
1101	43	2.3051411075308152
1101	44	0.8946438603607731
1101	45	1.9839644900306723
1101	46	2.5386327792577745
1101	47	2.3703305341173007
1101	48	1.829544396915722
1101	49	1.9356394116504418
1101	50	1.1368984841411347
1101	51	2.181459111422349
1101	52	-2.551695485886661
1101	53	0.2514324829582151
1101	54	1.0544063473291416
1101	55	2.15019669872612
1101	56	0.28585673585673277
1101	57	1.379271249583752
1101	58	1.1380808427683462
1101	59	-0.17146828865579522
1101	60	-0.4811025779775733
1101	61	-1.974987512487509
1101	62	-0.8665234071483994
1101	63	0.7887226777851808
1101	64	-0.975985993173488
1101	65	0.6293982059606975
1101	66	1.3474501771376808
1101	67	2.0419345122470176
1101	68	1.8956198893698897
1101	69	1.4057293979169003
1101	70	1.4671330983830941
1101	71	1.7377357017982007
1101	72	1.6902926356051395
1101	73	2.742733134920634
1101	74	-1.3273522773522828
1101	75	1.9379622113997144
1102	1	1.2838591963591988
1102	2	1.2582645120474005
1102	3	1.3646936275225663
1102	4	-0.28027369195132934
1102	5	-0.25906442485389647
1102	6	-0.5571729866137787
1102	7	0.7860686219238815
1102	8	-0.39936616745826825
1102	9	0.7315909815087522
1102	10	0.7256775449209627
1102	11	0.6352485812189741
1102	12	0.49070909597226375
1102	13	0.8402262698315255
1102	14	-0.14084815343038315
1102	15	1.590964968267599
1102	16	1.7830352913412142
1102	17	1.6101213905490184
1102	18	1.0739774206057078
1102	19	-0.3258335718862142
1102	20	0.9937032813677575
1102	21	1.6186207067786071
1102	22	1.8439898369174799
1102	23	1.1224197525184465
1102	24	1.63109567089829
1102	25	1.3515289109039088
1102	26	0.8006393490768531
1102	27	2.446319132256626
1102	28	2.3342502405002463
1102	29	-0.5527928090428134
1102	30	0.7499331108706073
1102	31	0.7645976130351144
1102	32	0.7838106916232022
1102	33	1.3696722259222156
1102	34	2.0853463087838193
1102	35	0.9352026445776573
1102	36	1.296173618048634
1102	37	1.1214018620268646
1102	38	-0.5031360421985411
1102	39	1.1323751480001576
1102	40	1.5647378016863414
1102	41	1.5210264102727393
1102	42	1.6734141709325456
1102	43	1.0687774711671807
1102	44	1.1310074967244077
1102	45	1.480934187000372
1102	46	1.5871176277426216
1102	47	-0.1084573446705761
1102	48	1.3628777302490533
1102	49	2.5356394116504433
1102	50	3.1368984841411347
1102	51	2.066307596270839
1102	52	1.9149711807800074
1102	53	1.415068846594579
1102	54	2.1695578624806515
1102	55	1.9198936684230858
1102	56	1.3767658267658263
1102	57	1.9117387820512874
1102	58	1.0082107128982187
1102	59	-0.6519877691752711
1102	60	-0.8577259546009515
1102	61	0.14189560439560722
1102	62	0.8023077616827692
1102	63	0.8211902102527162
1102	64	-0.15780417499166788
1102	65	1.1164111929736862
1102	66	1.5747229044104074
1102	67	0.17180464211714508
1102	68	-0.08489959114958623
1102	69	0.13300212518962695
1102	70	-1.0757240444740503
1102	71	-0.31940715534465625
1102	72	-0.0811359358234327
1102	73	-1.6341899420024433
1102	74	-0.3658138158138229
1102	75	0.6002998737373773
1103	1	0.7600496725496804
1103	2	1.0487407025235846
1103	3	1.3551698179987532
1103	4	0.3721072604296225
1103	5	0.8171260513365795
1103	6	0.3475889181481264
1103	7	1.205116240971499
1103	8	0.7530147849226836
1103	9	0.8506386005563726
1103	10	-0.43146531222188855
1103	11	0.3971533431237404
1103	12	-1.0569099516467872
1103	13	-0.6026308730256176
1103	14	1.1448661322839015
1103	15	-0.028082650780021368
1103	16	1.3068448151507326
1103	17	0.9958356762633045
1103	18	-0.29268924606095936
1103	19	-0.16392880998144932
1103	20	1.3413223289868057
1103	21	-0.09566500750710816
1103	22	-2.298867305939666
1103	23	0.5176578477565457
1103	24	0.178714718517341
1103	25	-0.39609013671513793
1103	26	0.04825839669589982
1103	27	0.09393817987567132
1103	28	1.4771073833573922
1103	29	2.066254810004807
1103	30	1.6070759680134614
1103	31	-0.5734976250601207
1103	32	1.0076202154327234
1103	33	0.07919603544603149
1103	34	0.6901082135457202
1103	35	1.9447264541014704
1103	36	2.400935522810535
1103	37	2.164259004884009
1103	38	-0.598374137436636
1103	39	1.5887854044104088
1103	40	0.9031993401478786
1103	41	1.3876930769394065
1103	42	3.1349526324710055
1103	43	1.9764697788594887
1103	44	2.454084419801326
1103	45	1.9629854690516524
1103	46	2.412758653383648
1103	47	2.978722142508907
1103	48	1.7526213199926417
1103	49	1.3202547962658286
1103	50	1.9830523302949814
1103	51	0.9227178526810889
1103	52	2.063689129497952
1103	53	2.9996842312099687
1103	54	2.543916836839628
1103	55	3.0942526427820596
1103	56	1.222919672919673
1103	57	1.9666838369963386
1103	58	1.403815108502613
1103	59	0.5348254176379115
1103	60	1.6587575618825667
1103	61	2.8012362637362713
1103	62	2.5110989704739737
1103	63	1.7607506498131542
1103	64	1.3806573634698722
1103	65	2.902125478687971
1103	66	2.459338289025794
1103	67	1.853123323435831
1103	68	1.8326828264328299
1103	69	2.3637713559588605
1103	70	0.6055946368446357
1103	71	1.8069664710289643
1103	72	-0.7020150567025567
1103	73	-0.48034378815628287
1103	74	0.4226477226477172
1103	75	0.8427241161616195
1104	1	0.2838591963592023
1104	2	0.5249311787140662
1104	3	1.1646936275225634
1104	4	0.6975040858264521
1104	5	1.2298244640349907
1104	6	1.6650492356084428
1104	7	0.8082908441461001
1104	8	0.8006338325417346
1104	9	1.1760354259532022
1104	10	1.2590108782543012
1104	11	0.41302635899675266
1104	12	1.2684868737500423
1104	13	0.9291151587204141
1104	14	0.7480407354585026
1104	15	0.9242983016009276
1104	16	1.227479735785657
1104	17	1.765676946104577
1104	18	2.0961996428279264
1104	19	1.2963886503360058
1104	20	1.460369948034426
1104	21	1.5075095956674929
1104	22	1.5106565035841442
1104	23	-1.8553580252593278
1104	24	-0.36890432910171
1104	25	-0.5151377557627583
1104	26	1.3339726824101845
1104	27	0.4907635767010703
1104	28	1.223139129389132
1104	29	1.7805405242905223
1104	30	2.083266444203943
1104	31	0.7201531685906701
1104	32	2.2060329138454264
1104	33	1.5474500036999999
1104	34	2.0631240865615936
1104	35	3.13520264457766
1104	36	2.8295069513819655
1104	37	1.965846306471306
1104	38	2.496863957801459
1104	39	3.110152925777932
1104	40	4.58696002390856
1104	41	2.3876930769394065
1104	42	2.451191948710324
1104	43	0.8909996933894035
1104	44	1.9754519411688491
1104	45	2.792045298111482
1104	46	2.942673183298176
1104	47	4.15820932199609
1104	48	0.7184332858046076
1104	49	3.2689727449837775
1104	50	2.5813429285855776
1104	51	2.1107520407152833
1104	52	1.7816378474466745
1104	53	0.9484021799279176
1104	54	2.458446751369543
1104	55	2.8976714462008673
1104	56	1.3767658267658263
1104	57	2.3085641788766864
1104	58	2.3097980144855157
1104	59	1.3638852466977411
1104	60	-0.7942338911088882
1104	61	0.6815781440781521
1104	62	-1.7453112859362818
1104	63	0.9402378293003366
1104	64	1.286640269452775
1104	65	-0.20898563242314339
1104	66	-0.5919437622562569
1104	67	0.6479951183076267
1104	68	1.2087512025012046
1104	69	2.3552243474118484
1104	70	-0.04397801272801871
1104	71	-1.5178198537573593
1104	72	-2.2319295866170847
1104	73	-3.182266865079363
1104	74	-0.07735227735228278
1104	75	1.176057449494948
1105	1	0.18862110112110742
1105	2	0.47731213109501525
1105	3	-1.1448301820012432
1105	4	-0.0564641681418081
1105	5	0.31712605133657945
1105	6	0.061874632433841725
1105	7	-0.36631233045707035
1105	8	0.11015764206554479
1105	9	-0.29221854230076616
1105	10	0.06853468777811145
1105	11	-0.1028466568762596
1105	12	0.08594719121035865
1105	13	0.25451198411724363
1105	14	-1.7122767248589525
1105	15	-0.17093979363716727
1105	16	0.5925591008650244
1105	17	0.1386928191204504
1105	18	-0.0069749603466746635
1105	19	-0.021071667124310522
1105	20	0.2698937575582363
1105	21	0.9757635639214612
1105	22	1.5582755512031952
1105	23	1.231943562042261
1105	24	1.3930004328030563
1105	25	1.8896241489991468
1105	26	1.5482583966958998
1105	27	1.5939381798756713
1105	28	2.4771073833573922
1105	29	2.3519690957190917
1105	30	1.8213616822991767
1105	31	1.8550738035113028
1105	32	0.1120158198283292
1105	33	1.667108123358119
1105	34	1.772525795963304
1105	35	0.4941770035520179
1105	36	1.137199259074272
1105	37	-0.09398275335774997
1105	38	0.7532742142117144
1105	39	0.9734007890257956
1105	40	2.8262762632247984
1105	41	3.61846230770864
1105	42	1.4426449401633121
1105	43	2.8995467019364156
1105	44	3.377161342878253
1105	45	1.5783700844362727
1105	46	0.7204509610759544
1105	47	1.363337527124294
1105	48	2.2141597815311087
1105	49	-1.9105144345034049
1105	50	0.290744637987288
1105	51	-0.9234359934727507
1105	52	0.525227591036419
1105	53	-1.4618542303284983
1105	54	0.7746860676088616
1105	55	0.4019449504743662
1105	56	1.1459965959965928
1105	57	-0.18716231684981466
1105	58	1.403815108502613
1105	59	-0.08055919774670173
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2306	3	-0.4305444677155279
2306	4	0.5149644032867684
2306	5	-0.04001680580628175
2306	6	0.13330320386241112
2306	7	0.7765448124000685
2306	8	-0.8898423579344552
2306	9	0.42206717198494204
2306	10	0.49710611634953494
2306	11	1.182867628838025
2306	12	1.2288043340674975
2306	13	1.0402262698315283
2306	14	1.4305804179981862
2306	15	0.543345920648548
2306	16	1.0211305294364479
2306	17	0.2101213905490198
2306	18	0.8501678967961865
2306	19	0.12178547573283538
2306	20	0.3413223289868057
2306	21	1.6900492782071765
2306	22	0.20113269406033396
2306	23	-0.6251992951006002
2306	24	1.678714718517341
2306	25	1.3181955775705774
2306	26	0.19111553955303862
2306	27	2.3082238941613866
2306	28	0.619964526214531
2306	29	1.566254810004807
2306	30	-1.8929240319865386
2306	31	-0.9306404822029819
2306	32	-2.4923797845672766
2306	33	-0.7065182502682603
2306	34	-4.167034643597134
2306	35	-0.2695592601842449
2306	36	0.3295069513819655
2306	37	0.9499732905982938
2306	38	0.8301972911347946
2306	39	-0.40572009009508037
2306	40	0.5075949445434844
2306	41	0.17340736265369117
2306	42	0.17341417093254563
2306	43	-0.04550824311853319
2306	44	-1.183278217561309
2306	45	-1.3666848606186761
2306	46	-0.2795490389240456
2306	47	0.3963045600913233
2306	48	0.7581158254871525
2306	49	1.0070679830790112
2306	50	1.4940413412839888
2306	51	0.8567837867470232
2306	52	0.09116165697048473
2306	53	-2.884931153405418
2306	54	-1.692346899424109
2306	55	0.9294174779468989
2306	56	-1.6232341732341737
2306	57	-1.0882612179487126
2306	58	0.9367821414696422
2306	59	0.062297945110444175
2306	60	0.7137026168276179
2306	61	-0.4295329670329622
2306	62	-1.873516414141406
2306	63	-1.5469416578791524
2306	64	-0.6193426365301278
2306	65	-0.6363360597735692
2306	66	-1.694507864820359
2306	67	-1.4545689842564755
2306	68	-0.62885563510563
2306	69	-1.328536336348833
2306	70	-1.702097670847671
2306	71	-1.7122642982018021
2306	72	-0.7369800916675899
2306	73	-0.35726686507936023
2306	74	-0.8273522773522828
2306	75	1.676057449494948
2307	1	1.017192529692533
2307	2	0.8915978453807334
2307	3	-3.6019730391441023
2307	4	-0.21360702528465936
2307	5	0.3742689084794364
2307	6	0.6761603467195556
2307	7	0.1194019552572172
2307	8	0.36730049920839747
2307	9	1.364924314842085
2307	10	1.025677544920967
2307	11	0.6685819145523126
2307	12	1.5573757626389266
2307	13	0.6402262698315297
2307	14	1.0591518465696197
2307	15	1.7576316349342633
2307	16	2.4497019580078785
2307	17	1.1101213905490184
2307	18	2.107310753939039
2307	19	1.3074997614471187
2307	20	0.1270366147010904
2307	21	0.31862070677861
2307	22	0.8439898369174799
2307	23	1.289086419185118
2307	24	1.9644290042316257
2307	25	1.6181955775705745
2307	26	1.0339726824101874
2307	27	0.7796524655899546
2307	28	-1.0657497594997523
2307	29	-0.6194594757094762
2307	30	-0.5500668891293898
2307	31	-0.0020690536315512986
2307	32	1.3504773582898721
2307	33	0.33633889258888416
2307	34	-0.5813203578828521
2307	35	-1.1981306887556755
2307	36	-1.570493048618033
2307	37	-0.17859813797313961
2307	38	-0.4698027088652026
2307	39	-0.7342915186665095
2307	40	-0.8352621983136643
2307	41	-1.9123069230605907
2307	42	-0.5265858290674572
2307	43	-1.3312225288328179
2307	44	0.03100749672440628
2307	45	0.24760085366703777
2307	46	-0.5795490389240427
2307	47	-1.375124011337249
2307	48	-3.170455603084278
2307	49	-2.1643605883495596
2307	50	-2.563101515858868
2307	51	-0.5003590703958309
2307	52	0.9483045141133388
2307	53	-2.184931153405415
2307	54	-2.0637754708526757
2307	55	1.4532270017564173
2307	56	-0.8732341732341737
2307	57	-0.6775469322344279
2307	58	-0.009646429958927172
2307	59	0.5444408022532983
2307	60	1.8029883311133332
2307	61	1.1954670329670378
2307	62	1.4630220473970539
2307	63	0.2319044959670009
2307	64	-0.15780417499166788
2307	65	-0.3478745213120291
2307	66	0.1997229044104074
2307	67	0.939661784974291
2307	68	-0.5134710197210168
2307	69	0.633002125189627
2307	70	0.5671330983830956
2307	71	1.7877357017981979
2307	72	-0.2597073643948633
2307	73	0.31773313492063693
2307	74	1.7440762940762866
2307	75	2.309390782828281
2308	1	0.9029068154068192
2308	2	1.3344549882378764
2308	3	1.2123126751416144
2308	4	1.0149644032867684
2308	5	0.8171260513365795
2308	6	1.4904460610052723
2308	7	1.6336876695429297
2308	8	1.324443356351253
2308	9	1.4934957434135185
2308	10	0.9256775449209655
2308	11	1.682867628838025
2308	12	2.014518619781782
2308	13	1.468797698402959
2308	14	1.5734375608553322
2308	15	1.1147744920771174
2308	16	-0.4074408991349756
2308	17	0.6386928191204504
2308	18	0.77873932536761
2308	19	2.1217854757328354
2308	20	2.2698937575582363
2308	21	-1.238522150364247
2308	22	0.27256126548891046
2308	23	0.017657847756545664
2308	24	1.3930004328030563
2308	25	2.3896241489991468
2308	26	3.3339726824101845
2308	27	4.093938179875671
2308	28	2.619964526214531
2308	29	3.3519690957190917
2308	30	3.7499331108706073
2308	31	2.9265023749398793
2308	32	3.5076202154327234
2308	33	3.4363388925888856
2308	34	2.332965356402866
2308	35	3.51615502553004
2308	36	3.400935522810535
2308	37	3.949973290598294
2308	38	3.401625862563364
2308	39	2.2371370527620584
2308	40	1.936166373114908
2308	41	2.816264505510837
2308	42	2.9591284566468303
2308	43	1.5259203283100362
2308	44	1.6738646395815522
2308	45	3.2047437108098933
2308	46	3.4347366753616697
2308	47	2.3963045600913233
2308	48	3.686687254058576
2308	49	2.792782268793296
2308	50	2.5654699127125653
2308	51	3.5710695010327385
2308	52	3.162590228399054
2308	53	3.757925989451728
2308	54	4.379081672004467
2308	55	3.643703192232607
2308	56	1.0910515410515416
2308	57	-3.516832646520143
2308	58	-0.2775035728160731
2308	59	-1.0805591977467017
2308	60	1.2851311882561873
2308	61	2.7133241758241837
2308	62	3.254688714063718
2308	63	1.9402378293003366
2308	64	0.8421958250083321
2308	65	-0.4312078546453648
2308	66	1.908056237743743
2308	67	2.6479951183076267
2308	68	1.6531956469456475
2308	69	0.9663354585229627
2308	70	1.5671330983830956
2308	71	2.204402368464862
2308	72	1.4902926356051367
2308	73	1.6093998015873012
2308	74	0.8393143893143815
2308	75	1.5093907828282838
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	7.0
23	11.0
24	15.0
25	44.0
26	70.0
27	91.0
28	96.0
29	153.0
30	141.0
31	219.0
32	217.0
33	268.0
34	276.0
35	297.0
36	445.0
37	593.0
38	682.0
39	846.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.238868172844924	11.34020618556701	19.280543978942752	40.14038166264532
2	22.582766937075203	19.995614996711247	31.177373383030037	26.24424468318351
3	29.24797193597895	22.955492216619163	23.70094277570708	24.095593071694804
4	27.406270554702918	31.857048892786672	18.41701381276036	22.319666739750055
5	28.151721113790835	30.958123218592416	21.0699408024556	19.820214865161148
6	23.31140350877193	34.56140350877193	20.67982456140351	21.44736842105263
7	21.859241394431045	17.868888401666304	38.719579039684284	21.552291164218374
8	25.54264415698312	21.815391361543522	24.90681868011401	27.73514580135935
9	22.10041657531243	21.771541328655996	28.23942117956588	27.88862091646569
10	23.30629247971936	38.566103924577945	20.67529050646788	17.452313089234817
11	28.85332163999123	26.50734488050866	18.50471387853541	26.134619600964697
12	25.08221881166411	23.196667397500548	26.178469633852224	25.54264415698312
13	23.13089234816926	27.93247094935321	27.011620258715197	21.92501644376233
14	24.29824561403509	27.039473684210524	26.92982456140351	21.732456140350877
15	25.871519403639553	24.358693269019952	25.783819337864504	23.98596798947599
16	25.498794124095593	24.7971935978952	25.433019074764307	24.270993203244903
17	26.77044507783381	26.353869765402322	25.213768910326685	21.661916246437187
18	26.331944748958563	26.814295110721332	23.679017759263317	23.174742381056788
19	26.61696996272747	25.76189432142074	24.687568515676386	22.9335672001754
20	27.16509537382153	25.16991887743916	24.643718482788863	23.02126726595045
21	24.007022163704193	26.991441737985518	26.771999122229538	22.229536976080755
22	25.065905096660806	26.340070298769767	25.48330404217926	23.110720562390156
23	26.455724016699627	26.785321907273126	24.082619204570424	22.67633487145682
24	23.784378437843785	27.590759075907588	25.47854785478548	23.146314631463145
25	25.561921551344202	25.628029969149406	24.9008373732922	23.909211106214194
26	25.17683465959328	26.8788682581786	23.762157382847036	24.18213969938108
27	27.7212389380531	25.818584070796458	24.491150442477878	21.969026548672566
28	26.578073089701	26.223698781838316	25.315614617940202	21.88261351052049
29	24.196052339764915	26.813040585495674	26.43601685517853	22.55489021956088
30	26.249167221852097	26.093715300910503	24.361536753275594	23.295580723961802
31	26.90253671562083	24.922118380062305	25.656430796617713	22.518914107699153
32	23.277591973244146	25.08361204013378	27.224080267558527	24.414715719063544
33	24.011614920705828	26.33459906187179	26.89300871119053	22.76077730623185
34	24.871393424289867	25.497651532095727	25.833146946991725	23.79780809662268
35	24.472857783759533	25.370121130551816	26.132794975325258	24.024226110363394
36	22.961132329813523	24.80341496292968	27.027634239496745	25.207818467760053
37	22.927927927927925	24.054054054054056	25.96846846846847	27.04954954954955
38	22.988245931283906	25.31645569620253	28.028933092224236	23.66636528028933
39	22.73035997283224	25.85465247905818	26.91872311523659	24.49626443287299
40	22.942643391521198	25.66311493992292	27.22738608025391	24.16685558830197
41	22.863636363636363	27.045454545454543	25.886363636363637	24.204545454545453
42	25.187713310580207	26.96245733788396	25.756541524459614	22.09328782707622
43	25.49019607843137	26.65298677610579	25.421796625626996	22.43502051983584
44	25.228310502283108	25.525114155251146	24.794520547945208	24.452054794520546
45	26.06310013717421	26.26886145404664	25.90306355738454	21.764974851394605
46	24.232003668042182	26.5474552957359	26.15772581384686	23.062815222375058
47	22.796143250688704	26.30853994490358	25.872359963269055	25.02295684113866
48	24.20689655172414	25.080459770114942	25.126436781609197	25.586206896551722
49	22.940635066728028	27.381500230096638	26.06994937873907	23.607915324436263
50	25.13812154696133	26.611418047882136	26.151012891344383	22.099447513812155
51	21.710071445033417	26.135054159944687	27.33348697856649	24.821387416455405
52	23.192423192423192	25.594825594825593	26.357126357126358	24.855624855624857
53	22.142526608051828	26.839426191577974	25.59000462748727	25.428042572882926
54	23.771827706635623	25.07566938300349	26.16996507566938	24.982537834691502
55	21.529411764705884	25.176470588235293	27.48235294117647	25.81176470588235
56	22.74569402228977	26.72239108409321	27.583586626139816	22.9483282674772
57	23.212013234919826	26.902519725120893	27.309748027487913	22.575719012471367
58	20.968152866242036	28.53503184713376	26.547770700636942	23.949044585987263
59	22.310045894951553	28.17440081591025	27.001529831718514	22.514023457419686
60	22.434915773353755	28.458397141398674	27.36089841755998	21.745788667687595
61	23.95700025595086	26.490913744561045	28.666496032761714	20.88558996672639
62	22.638603696098563	27.15605749486653	27.951745379876797	22.253593429158112
63	23.177955189286635	26.654648467679632	28.714911151171773	21.45248519186196
64	23.43225806451613	26.606451612903225	26.270967741935486	23.690322580645162
65	21.692984726896196	28.656484597463113	26.58555526792648	23.06497540771421
66	24.20779220779221	26.1038961038961	27.558441558441558	22.12987012987013
67	22.764015645371575	27.77053455019557	27.926988265971314	21.53846153846154
68	23.121235925634984	26.760932181199266	26.630007855459542	23.487824037706208
69	22.854133754607687	25.934702474986835	28.17272248551869	23.038441284886783
70	22.21044261860588	27.140206732043463	27.16671084018023	23.482639809170422
71	21.203208556149733	27.727272727272727	28.903743315508024	22.165775401069517
72	21.813261163734776	28.03788903924222	27.198917456021647	22.949932341001354
73	23.608391608391607	26.461538461538463	27.804195804195803	22.125874125874127
74	21.914050594330998	24.657116732703443	29.625114294422435	23.803718378543127
75	23.544743701129452	0.0	41.398783666377064	35.05647263249349
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	0.5
11	0.5
12	2.0
13	3.5
14	5.5
15	6.5
16	5.0
17	7.0
18	12.0
19	15.5
20	16.0
21	13.0
22	21.0
23	31.5
24	36.5
25	48.5
26	55.0
27	61.0
28	70.5
29	90.0
30	109.0
31	121.5
32	126.0
33	127.0
34	135.0
35	148.0
36	156.5
37	154.0
38	156.0
39	152.5
40	149.0
41	143.0
42	167.0
43	179.0
44	174.5
45	172.0
46	158.0
47	156.0
48	156.5
49	160.0
50	165.5
51	168.5
52	162.5
53	158.0
54	156.0
55	160.5
56	159.5
57	143.5
58	123.0
59	130.0
60	144.5
61	129.0
62	122.0
63	108.5
64	85.5
65	77.5
66	66.5
67	54.0
68	40.0
69	38.0
70	36.0
71	25.5
72	23.5
73	22.0
74	15.0
75	13.5
76	17.0
77	15.5
78	22.5
79	32.0
80	38.0
81	48.5
82	52.5
83	134.5
84	211.5
85	115.0
86	14.5
87	3.5
88	1.0
89	0.5
90	1.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04385003288752467
2	0.0
3	0.0
4	0.0
5	0.0
6	0.021925016443762334
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.021925016443762334
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.02544529262086514
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.025879917184265012
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	9.0
22-23	7.0
24-25	21.0
26-27	9.0
28-29	12.0
30-31	18.0
32-33	14.0
34-35	20.0
36-37	27.0
38-39	13.0
40-41	16.0
42-43	15.0
44-45	18.0
46-47	12.0
48-49	6.0
50-51	15.0
52-53	34.0
54-55	347.0
56-57	23.0
58-59	7.0
60-61	22.0
62-63	21.0
64-65	25.0
66-67	31.0
68-69	46.0
70-71	78.0
72-73	414.0
74-75	3281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.5454944091208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.4126984126984	91.07651830738874
2	0.6870409855484482	1.2716509537382152
3	0.37905709547500593	1.052400789300592
4	0.04738213693437574	0.17540013155009868
5	0.16583747927031509	0.7673755755316817
6	0.04738213693437574	0.263100197325148
7	0.0	0.0
8	0.02369106846718787	0.17540013155009868
9	0.02369106846718787	0.19732514799386097
>10	0.18952854773750297	3.6395527296645467
>50	0.02369106846718787	1.3812760359570269
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGC	63	1.3812760359570269	No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC	44	0.9647007235255427	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	35	0.7673755755316817	No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCC	25	0.5481254110940583	No Hit
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC	19	0.4165753124314843	No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC	12	0.263100197325148	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	11	0.24117518088138568	No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC	10	0.21925016443762332	No Hit
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT	10	0.21925016443762332	No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG	9	0.19732514799386097	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	8	0.17540013155009868	No Hit
GAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGA	6	0.131550098662574	No Hit
GGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCC	6	0.131550098662574	No Hit
CCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	5	0.10962508221881166	No Hit
CAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	5	0.10962508221881166	No Hit
CCGAGAGGCAAGGGGCGGGGACGGG	5	0.10962508221881166	No Hit
CAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCG	5	0.10962508221881166	No Hit
GCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCG	5	0.10962508221881166	No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	5	0.10962508221881166	No Hit
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTC	5	0.10962508221881166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
