##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	B-1-R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4561
Sequences flagged as poor quality	0
Sequence length	20-75
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.521596141197104	31.0	31.0	34.0	26.0	34.0
2	30.828984871738655	31.0	31.0	34.0	27.0	34.0
3	30.91427318570489	31.0	31.0	34.0	26.0	34.0
4	34.41328655996492	37.0	35.0	37.0	30.0	37.0
5	34.383030037272526	37.0	35.0	37.0	30.0	37.0
6	34.32098224073668	37.0	35.0	37.0	30.0	37.0
7	34.221442666082	37.0	35.0	37.0	30.0	37.0
8	34.31023898267924	37.0	35.0	37.0	29.0	37.0
9	35.831835123876346	39.0	35.0	39.0	30.0	39.0
10	35.815829861872395	39.0	35.0	39.0	30.0	39.0
11	35.967331725498795	39.0	35.0	39.0	30.0	39.0
12	35.71080903310678	39.0	35.0	39.0	29.0	39.0
13	35.520061390046045	39.0	35.0	39.0	27.0	39.0
14	36.78184608638456	40.0	36.0	41.0	29.0	41.0
15	36.39574654680991	39.0	36.0	41.0	27.0	41.0
16	36.69436527077395	39.0	36.0	41.0	29.0	41.0
17	36.736022802017104	39.0	36.0	41.0	30.0	41.0
18	36.74040780530586	39.0	36.0	41.0	30.0	41.0
19	36.81429511072133	40.0	36.0	41.0	29.0	41.0
20	36.695461521596144	39.0	36.0	41.0	29.0	41.0
21	36.45814106789717	39.0	35.0	41.0	27.0	41.0
22	36.44292940400264	39.0	35.0	41.0	28.0	41.0
23	36.37006832708838	39.0	35.0	41.0	27.0	41.0
24	36.48541758727353	39.0	35.0	41.0	28.0	41.0
25	36.205224706663714	39.0	35.0	41.0	27.0	41.0
26	36.30133333333333	39.0	35.0	41.0	27.0	41.0
27	36.26631039857493	39.0	35.0	41.0	27.0	41.0
28	36.271957200178335	39.0	35.0	41.0	27.0	41.0
29	36.07063030844881	39.0	35.0	41.0	27.0	41.0
30	36.004927211646134	39.0	34.0	41.0	27.0	41.0
31	36.06219128872923	39.0	35.0	41.0	27.0	41.0
32	35.758667266996845	39.0	34.0	41.0	26.0	41.0
33	35.938600451467266	39.0	34.0	41.0	26.0	41.0
34	36.04870865428183	39.0	34.0	41.0	27.0	41.0
35	36.11191827468785	38.0	34.0	41.0	27.0	41.0
36	36.15312855517634	38.0	35.0	40.0	27.0	41.0
37	31.037934186471663	38.0	29.0	40.0	2.0	41.0
38	32.63190465276186	38.0	28.0	40.0	15.0	41.0
39	34.263556985294116	38.0	30.0	40.0	23.0	41.0
40	34.95651173492867	38.0	33.0	40.0	25.0	41.0
41	35.36275639548283	38.0	33.0	40.0	25.0	41.0
42	35.46948682385575	38.0	34.0	40.0	25.0	41.0
43	35.45542949756888	38.0	33.0	40.0	25.0	41.0
44	35.485130111524164	38.0	34.0	40.0	26.0	41.0
45	35.574819641610425	38.0	34.0	40.0	26.0	41.0
46	35.522753792298715	38.0	33.0	40.0	26.0	41.0
47	35.29588014981273	38.0	33.0	40.0	25.0	41.0
48	35.435506566604126	38.0	33.0	40.0	26.0	41.0
49	35.67224316682375	38.0	33.0	40.0	27.0	41.0
50	35.5747099218565	38.0	34.0	40.0	27.0	41.0
51	34.09749103942652	37.0	32.0	39.0	24.0	40.0
52	34.40991854336368	37.0	32.0	39.0	25.0	40.0
53	35.27180966113915	38.0	33.0	40.0	27.0	41.0
54	35.548621190130625	38.0	33.0	40.0	27.0	41.0
55	35.841394549472135	38.0	34.0	40.0	28.0	41.0
56	36.22955561398896	38.0	34.0	40.0	29.0	41.0
57	36.240526315789474	38.0	35.0	40.0	29.0	41.0
58	36.02264946010008	38.0	34.0	40.0	29.0	41.0
59	36.029031406703616	38.0	34.0	40.0	29.0	41.0
60	35.89933949801849	38.0	34.0	40.0	29.0	41.0
61	35.87748081503043	38.0	34.0	40.0	28.0	41.0
62	35.729199788023315	37.0	34.0	40.0	29.0	41.0
63	35.60806152214267	37.0	34.0	40.0	28.0	41.0
64	35.535970268117865	37.0	34.0	40.0	29.0	41.0
65	35.48894806924101	37.0	34.0	40.0	28.0	41.0
66	35.464905257539364	36.0	34.0	40.0	29.0	41.0
67	35.15645894624231	36.0	33.0	39.0	28.0	41.0
68	35.044808156694394	36.0	33.0	39.0	28.0	41.0
69	35.148517520215634	36.0	34.0	39.0	29.0	41.0
70	35.2520325203252	36.0	34.0	39.0	29.0	41.0
71	35.259410801963995	36.0	34.0	39.0	30.0	41.0
72	35.2734783806114	35.0	34.0	39.0	30.0	41.0
73	35.35212464589235	35.0	34.0	39.0	30.0	41.0
74	35.32275777913362	35.0	34.0	39.0	30.0	40.0
75	34.779632006846384	35.0	33.0	37.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1527304384212265
1101	2	1.9175821863321936
1101	3	1.6004135788675207
1101	4	1.6322812360641237
1101	5	2.123672921863701
1101	6	2.2291446035689546
1101	7	1.1230462154804215
1101	8	2.1356078893085453
1101	9	3.1579771592929475
1101	10	3.064884278124417
1101	11	2.607110229560888
1101	12	2.8168679273942487
1101	13	2.7406076866603257
1101	14	3.542520168671473
1101	15	3.4107494996475225
1101	16	3.251314754933162
1101	17	2.969218117408907
1101	18	3.4041084555064884
1101	19	2.771003999046769
1101	20	3.150005181830828
1101	21	1.9206287316484776
1101	22	2.5870940158769216
1101	23	2.0776952366590535
1101	24	2.6947857332725675
1101	25	2.023740264365273
1101	26	2.8129853820643262
1101	27	3.393686050182758
1101	28	3.9452153049192518
1101	29	4.045049294638119
1101	30	3.06630621145424
1101	31	4.124383718133707
1101	32	3.2219161381990347
1101	33	3.5755500256322676
1101	34	4.3022785724266015
1101	35	3.8903398197806
1101	36	4.0623307735149865
1101	37	9.351536307868546
1101	38	7.564049099657652
1101	39	5.533866252534018
1101	40	5.357798162485665
1101	41	3.978765316676487
1101	42	4.297659988038269
1101	43	3.5329618706456927
1101	44	3.6001193319208014
1101	45	1.7694023569023543
1101	46	3.435473987817737
1101	47	4.094549663299659
1101	48	3.6675974777537164
1101	49	3.200382545695035
1101	50	3.252243416305909
1101	51	3.2284421596921646
1101	52	3.5042403198653247
1101	53	2.9213023088023107
1101	54	3.320344759407263
1101	55	2.6540028836903815
1101	56	1.680882485569981
1101	57	2.28017751924002
1101	58	2.354153138528133
1101	59	2.584296837421853
1101	60	1.7577734187109186
1101	61	2.448293951418947
1101	62	1.980858435545933
1101	63	2.2731586700336734
1101	64	2.3932509620009625
1101	65	1.4680698352573387
1101	66	-0.11302985209235317
1101	67	1.281885070947574
1101	68	1.4815438612313727
1101	69	1.6073759573759574
1101	70	1.2082763879638776
1101	71	1.3622908919783967
1101	72	1.7368070586820608
1101	73	1.4664411976911893
1101	74	1.9504979973729917
1101	75	2.5771825396825463
1102	1	1.292124377815167
1102	2	1.9963700651200718
1102	3	1.7458681243220688
1102	4	2.0989479027307922
1102	5	1.0206426188333992
1102	6	0.5442961187204673
1102	7	1.6866825791167912
1102	8	1.7659109196115779
1102	9	1.7640377653535566
1102	10	1.5436721569122938
1102	11	2.104079926530588
1102	12	2.7259588364851552
1102	13	2.8436379896906274
1102	14	3.0940353201866273
1102	15	2.35014343904146
1102	16	2.5240420276604354
1102	17	2.9995211477119383
1102	18	2.0707751221731527
1102	19	3.055852483895258
1102	20	2.6469748788005276
1102	21	4.029719640739387
1102	22	1.496184924967828
1102	23	3.0655740245378382
1102	24	3.603876642363474
1102	25	1.5085887492137573
1102	26	2.379652048730989
1102	27	3.7603527168494253
1102	28	2.545215304919253
1102	29	3.345049294638116
1102	30	3.5996395447875784
1102	31	3.557717051467044
1102	32	3.6552494715323647
1102	33	1.5422166922989362
1102	34	2.835611905759933
1102	35	1.690339819780597
1102	36	2.3623307735149908
1102	37	8.51820297453521
1102	38	6.53071576632432
1102	39	5.033866252534018
1102	40	3.9244648291523276
1102	41	3.0787653166764883
1102	42	2.497659988038272
1102	43	3.3662952039790284
1102	44	2.7667859985874728
1102	45	4.236069023569016
1102	46	4.0021406544844
1102	47	1.0278829966329894
1102	48	2.7342641444203863
1102	49	2.9337158790283695
1102	50	2.4522434163059117
1102	51	2.695108826358826
1102	52	3.104240319865326
1102	53	3.0546356421356435
1102	54	1.55367809274059
1102	55	2.3540028836903772
1102	56	1.4808824855699783
1102	57	1.8658918049543018
1102	58	2.0398674242424164
1102	59	2.4557254088504195
1102	60	2.329201990139488
1102	61	1.7054368085617995
1102	62	1.2237155784030804
1102	63	-0.35541275853776
1102	64	-0.9353204665704666
1102	65	0.4680698352573387
1102	66	0.04411300505049809
1102	67	0.9104564995190074
1102	68	0.9958295755170852
1102	69	0.6073759573759574
1102	70	0.40827638796388044
1102	71	1.04800517769268
1102	72	0.7510927729677732
1102	73	0.38072691197690745
1102	74	0.7966518435268384
1102	75	1.410515873015882
1103	1	0.9254577111484998
1103	2	1.6058938746438862
1103	3	-0.06841758996364788
1103	4	0.20370980749269307
1103	5	1.0587378569286372
1103	6	1.5538199282442804
1103	7	1.2009682934025037
1103	8	1.7849585386591968
1103	9	1.787847289163075
1103	10	2.0389102521503943
1103	11	1.6850323074829703
1103	12	1.9402445507708705
1103	13	2.376971323023959
1103	14	1.360701986853293
1103	15	2.54061962951765
1103	16	1.5240420276604354
1103	17	1.1185687667595587
1103	18	2.332679884077919
1103	19	1.8034715315143046
1103	20	1.8707844026100489
1103	21	1.0440053550250994
1103	22	1.2104706392535434
1103	23	-0.4677593087954932
1103	24	-0.39612335763652595
1103	25	0.7704935111185236
1103	26	-0.0013003322213904767
1103	27	1.3936860501827582
1103	28	2.4452153049192518
1103	29	2.2736207232095467
1103	30	1.8377347828828121
1103	31	0.0815265752765626
1103	32	0.05048756677046384
1103	33	0.6041214542036997
1103	34	-1.0405785704305472
1103	35	-0.32394589450511546
1103	36	-0.7662406550564427
1103	37	4.96582202215426
1103	38	3.9640490996576503
1103	39	1.9910091096768738
1103	40	0.6149410196285174
1103	41	-2.906948969037799
1103	42	-0.6737685833902987
1103	43	-0.1527524150685906
1103	44	0.4144050462065181
1103	45	0.9265452140452055
1103	46	-1.0502402978965506
1103	47	-0.6483074795574879
1103	48	0.7675974777537178
1103	49	-1.2996174543049648
1103	50	0.7522434163059089
1103	51	0.3855850168350159
1103	52	0.1470974627224706
1103	53	0.021302308802312098
1103	54	0.38957552863802647
1103	55	0.18477211445961217
1103	56	2.844518849206345
1103	57	2.0347229737854704
1103	58	1.7541531385281317
1103	59	1.5206604737854832
1103	60	0.3032279641654654
1103	61	2.6210212241462187
1103	62	0.9444947991823014
1103	63	1.845885942760944
1103	64	3.0387055074555107
1103	65	1.1953425625300653
1103	66	2.1415156024530972
1103	67	3.1637032527657567
1103	68	3.0088165885040965
1103	69	1.4710123210123243
1103	70	1.999185478872974
1103	71	0.3986545283420284
1103	72	1.4458979677729644
1103	73	0.848259379509372
1103	74	0.8595889064638982
1103	75	0.8390873015873126
1104	1	-0.6856533999626109
1104	2	-0.42585215710214896
1104	3	0.47920145765539957
1104	4	-1.0343854306025406
1104	5	0.4873092855000678
1104	6	-1.2557038812795298
1104	7	-1.4022063097720974
1104	8	-2.7229779692773093
1104	9	-0.013740012424221959
1104	10	-0.5007722875321505
1104	11	-1.4736978512471879
1104	12	0.1704032809295981
1104	13	-0.28969534364270544
1104	14	0.02736865351995732
1104	15	-0.20541211651408986
1104	16	0.4129309165493211
1104	17	-1.667145518954726
1104	18	0.9596640110620456
1104	19	-0.4108141827714107
1104	20	-0.37524734342169097
1104	21	1.7186085296282698
1104	22	1.940629369412271
1104	23	0.6433518023156211
1104	24	0.27054330903013835
1104	25	3.0641443047693144
1104	26	2.462985382064325
1104	27	3.893686050182758
1104	28	2.8202153049192518
1104	29	3.720049294638116
1104	30	3.1413062114542427
1104	31	3.2243837181337085
1104	32	2.2469161381990332
1104	33	1.550550025632269
1104	34	3.6022785724265987
1104	35	2.7653398197806
1104	36	2.287330773514988
1104	37	8.251536307868545
1104	38	6.96404909965765
1104	39	4.13386625253402
1104	40	4.007798162485663
1104	41	3.7537653166764855
1104	42	3.3976599880382707
1104	43	3.882961870645694
1104	44	2.825119331920803
1104	45	3.4444023569023514
1104	46	4.360473987817734
1104	47	1.119549663299658
1104	48	2.392597477753718
1104	49	3.075382545695035
1104	50	3.627243416305909
1104	51	3.528442159692162
1104	52	3.5042403198653247
1104	53	3.396302308802312
1104	54	3.87034475940726
1104	55	2.8290028836903787
1104	56	1.9808824855699783
1104	57	1.5801775192400171
1104	58	2.8791531385281317
1104	59	3.13429683742185
1104	60	3.0077734187109186
1104	61	4.223293951418945
1104	62	2.5808584355459345
1104	63	2.0731586700336706
1104	64	2.493250962000964
1104	65	1.8430698352573387
1104	66	2.311970147907644
1104	67	1.8568850709475768
1104	68	2.65654386123137
1104	69	2.6073759573759574
1104	70	3.5332763879638804
1104	71	2.8872908919783953
1104	72	3.036807058682058
1104	73	2.416441197691192
1104	74	2.9504979973729917
1104	75	2.5771825396825463
1105	1	1.7111719968627845
1105	2	0.6058938746438827
1105	3	0.28872526717920977
1105	4	0.7751383789212696
1105	5	0.6301664283572137
1105	6	-0.23189435747001141
1105	7	1.5581111505453578
1105	8	1.8563871100877662
1105	9	1.5735615748773668
1105	10	1.1817673950075331
1105	11	1.470746593197255
1105	12	-0.5597554492291295
1105	13	1.5198284658811048
1105	14	0.4321305582818624
1105	15	1.6120482009462265
1105	16	0.5240420276604354
1105	17	1.9042830524738434
1105	18	1.2612513126493425
1105	19	2.1606143886571587
1105	20	1.5850701168957642
1105	21	1.5440053550250994
1105	22	1.0676134963963975
1105	23	2.3179549769187915
1105	24	1.8181623566491893
1105	25	2.1276363682613777
1105	26	1.8558425249214707
1105	27	1.536543193039904
1105	28	1.8023581620621059
1105	29	1.4164778660666855
1105	30	0.3377347828828121
1105	31	2.0815265752765626
1105	32	-0.02094100465811266
1105	33	0.5326928827751232
1105	34	2.3879928581408834
1105	35	2.4617683912091692
1105	36	2.805187916372134
1105	37	7.751536307868545
1105	38	4.606906242514796
1105	39	3.7767233953911656
1105	40	2.043512448199948
1105	41	1.0930510309622008
1105	42	2.754802845181125
1105	43	-1.4384667007828753
1105	44	0.2715479033493722
1105	45	1.2122594997594973
1105	46	-0.12166886932512
1105	47	1.851692520442512
1105	48	-0.5181168079605669
1105	49	0.5575254028378893
1105	50	-0.890613726551237
1105	51	-1.257272126022123
1105	52	-0.4957596801346753
1105	53	0.7355880230880274
1105	54	0.5489161879786906
1105	55	-0.9031399734524754
1105	56	-1.1619746572871676
1105	57	-0.34839390933141345
1105	58	-0.4601325757575836
1105	59	-2.472846019721004
1105	60	-2.3136551527176508
1105	61	-1.1517060485810546
1105	62	-1.1334272787397808
1105	63	-1.7839841871091835
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2306	24	-1.5389805004936719
2306	25	-0.9437922031671917
2306	26	-0.4298717607928211
2306	27	-0.32059966410295715
2306	28	-1.1976418379378941
2306	29	-0.44066499107616863
2306	30	0.2663062114542427
2306	31	0.5815265752765626
2306	32	-0.8780838618009668
2306	33	-1.5387356886534462
2306	34	1.4868939570419855
2306	35	-2.8788909494501667
2306	36	0.08540769659190772
2306	37	6.174613230945464
2306	38	2.0794337150422635
2306	39	-0.5969029782352138
2306	40	1.0270289317164298
2306	41	1.186457624368792
2306	42	-2.1023400119617293
2306	43	0.4021926398764606
2306	44	-0.3768037450022774
2306	45	0.5694023569023514
2306	46	0.0431662955100407
2306	47	-0.255450336700342
2306	48	0.18426414442038208
2306	49	1.2003825456950352
2306	50	-0.5810899170274268
2306	51	-1.554891173641174
2306	52	-0.579093013468011
2306	53	-2.145364357864352
2306	54	-2.12965524059274
2306	55	-0.37933044964295703
2306	56	-0.8827538780663886
2306	57	0.21654115560365028
2306	58	1.5723349567099518
2306	59	1.7933877465127566
2306	60	-0.33313567219817486
2306	61	-0.7426151394901481
2306	62	-1.2373233826358856
2306	63	-0.4268413299663294
2306	64	0.1296145983645971
2306	65	-1.4410210738335678
2306	66	0.686970147907644
2306	67	-0.6544785654160563
2306	68	-1.7184561387686301
2306	69	-2.6198967698967692
2306	70	-0.7280872483997527
2306	71	-0.23770910802160472
2306	72	-0.2813747594997622
2306	73	1.030077561327559
2306	74	-0.776774729899735
2306	75	-0.2561507936507823
2307	1	1.1254577111484991
2307	2	1.5630367317867417
2307	3	1.8458681243220703
2307	4	2.432281236064121
2307	5	1.3873092855000664
2307	6	1.6109627853871373
2307	7	0.8866825791167869
2307	8	1.5992442529449136
2307	9	2.8307044320202195
2307	10	2.6103388235789637
2307	11	1.970746593197255
2307	12	3.425958836485158
2307	13	-0.02302867697603972
2307	14	2.860701986853293
2307	15	3.983476772374793
2307	16	2.3240420276604326
2307	17	2.4328544810452755
2307	18	2.00410845550649
2307	19	0.7891858172285922
2307	20	1.2136415454671976
2307	21	3.2297196407393827
2307	22	2.496184924967828
2307	23	3.332240691204504
2307	24	3.0038766423634726
2307	25	2.341922082547093
2307	26	-1.5870146179356723
2307	27	-0.5063139498172404
2307	28	-0.6547846950807497
2307	29	-0.4549507053618811
2307	30	2.06630621145424
2307	31	2.32438371813371
2307	32	0.22191613819903466
2307	33	-0.4355610854788452
2307	34	2.213389683537713
2307	35	1.7792287086694856
2307	36	2.606775217959431
2307	37	5.473758530090766
2307	38	5.686271321879872
2307	39	3.744977363645134
2307	40	0.4800203847078848
2307	41	3.212098650009821
2307	42	2.8976599880382707
2307	43	1.9662952039790298
2307	44	3.1445637763652456
2307	45	3.34718013468013
2307	46	1.2910295433732912
2307	47	2.1612163299663223
2307	48	2.1564863666426035
2307	49	0.20038254569503522
2307	50	-0.9699788059163126
2307	51	-0.8604467291967239
2307	52	0.9486847643097676
2307	53	0.6324134199134264
2307	54	1.5092336482961457
2307	55	-0.6015526718651785
2307	56	0.9808824855699783
2307	57	0.7051775192400171
2307	58	-0.8708468614718683
2307	59	0.38429683742185006
2307	60	0.8827734187109186
2307	61	-1.9017060485810546
2307	62	-0.16914156445406547
2307	63	0.1981586700336706
2307	64	0.868250962000964
2307	65	-0.1569301647426613
2307	66	1.061970147907644
2307	67	0.7318850709475768
2307	68	-0.09345613876863013
2307	69	-0.6426240426240426
2307	70	-0.09172361203611956
2307	71	0.3872908919783953
2307	72	0.2868070586820579
2307	73	0.9164411976911921
2307	74	0.664783711658707
2307	75	-2.089484126984118
2308	1	0.8540291397199269
2308	2	1.748751017501025
2308	3	2.1458681243220674
2308	4	2.0608526646355543
2308	5	1.9158807140714984
2308	6	0.5538199282442804
2308	7	1.915254007688219
2308	8	0.49924425294491215
2308	9	2.0021330034487903
2308	10	2.6103388235789637
2308	11	1.970746593197255
2308	12	2.4402445507708705
2308	13	1.234114180166813
2308	14	2.6464162725675777
2308	15	1.6834767723747959
2308	16	1.5240420276604354
2308	17	0.33285448104527404
2308	18	-2.167320115922081
2308	19	0.874900102942874
2308	20	-0.48635845453280524
2308	21	-0.7417089306891853
2308	22	-1.360957932175026
2308	23	0.2465264054902221
2308	24	0.46101949950632815
2308	25	2.2704935111185236
2308	26	1.1415568106357554
2308	27	1.7508289073256122
2308	28	0.9452153049192518
2308	29	1.345049294638116
2308	30	-0.5908366456886114
2308	31	0.9386694324194238
2308	32	2.1219161381990332
2308	33	1.675550025632269
2308	34	0.5308500009980293
2308	35	1.4617683912091692
2308	36	-0.980526369342158
2308	37	4.96582202215426
2308	38	4.321191956800504
2308	39	0.49100910967687383
2308	40	0.8292267339142327
2308	41	0.4501938881050549
2308	42	0.04051713089541664
2308	43	-1.9384667007828753
2308	44	-0.014166382364912522
2308	45	0.1408309283309208
2308	46	0.021188273532018798
2308	47	1.637406806156804
2308	48	3.124740334896572
2308	49	0.4860968314093199
2308	50	1.3951005591630548
2308	51	2.028442159692162
2308	52	0.5042403198653247
2308	53	2.0927308802308815
2308	54	2.3346304736929753
2308	55	2.525431455118948
2308	56	2.6475491522366426
2308	57	1.7468441859066814
2308	58	1.420819805194796
2308	59	2.7176301707551858
2308	60	1.924440085377583
2308	61	2.5149606180856097
2308	62	2.9141917688792702
2308	63	2.739825336700335
2308	64	1.3265842953342997
2308	65	1.9680698352573387
2308	66	1.8536368145743083
2308	67	2.3152184042809125
2308	68	2.1148771945647056
2308	69	2.940709290709293
2308	70	1.7416097212972161
2308	71	1.595624225311731
2308	72	1.8701403920153936
2308	73	0.16644119769119214
2308	74	1.2838313307063274
2308	75	0.5771825396825463
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	14.0
20	40.0
21	46.0
22	54.0
23	41.0
24	62.0
25	68.0
26	76.0
27	83.0
28	118.0
29	125.0
30	131.0
31	159.0
32	197.0
33	227.0
34	311.0
35	310.0
36	438.0
37	592.0
38	666.0
39	707.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.342480790340286	12.074643249176729	18.507135016465423	41.07574094401756
2	24.007893005919755	20.19294014470511	30.234597675948255	25.564569173426882
3	28.94102170576628	23.810567857925893	22.516991887743913	24.73141854856391
4	29.42337206752905	29.97149747862311	18.219688664766498	22.38544178908134
5	29.401447051085288	30.6730980048235	20.916465687349266	19.00898925674194
6	22.18811664108748	35.40890155667617	20.236790177592635	22.16619162464372
7	21.376891032668276	17.386538039903527	39.72812979609734	21.508441131330848
8	23.328217496163123	21.53036614777461	26.39771979828985	28.74369655777242
9	22.47314185485639	22.802017101512824	28.919096689322515	25.805744354308263
10	23.437842578381936	37.27252795439597	21.0699408024556	18.219688664766498
11	28.699846524884894	25.58649418987064	18.855514141635606	26.85814514360886
12	24.31484323613243	22.363516772637578	27.976320982240736	25.345319008989257
13	23.98596798947599	26.96777022582767	27.274720456040342	21.771541328655996
14	24.24906818680114	27.53782065336549	26.375794781846086	21.837316377987285
15	25.433019074764307	26.11269458452094	25.323393992545494	23.13089234816926
16	25.718044288533214	24.336768252576192	26.3100197325148	23.635167726375794
17	26.57311992983995	26.726595044946283	23.59131769348827	23.1089673317255
18	25.23569392677045	26.989695242271434	24.07366805525104	23.70094277570708
19	25.23569392677045	26.331944748958563	24.468318351238764	23.964042973032228
20	26.00306950230213	27.581670686253013	24.336768252576192	22.078491558868667
21	24.104592397275322	28.301472203911228	24.675895407602724	22.918039991210723
22	24.477677589619528	26.171101825379374	25.62128876182098	23.72993182318012
23	25.611637646021602	27.46308133127617	23.91448093453824	23.010800088163986
24	25.45293857711003	27.86124613345117	24.812196199734863	21.873619089703933
25	25.75354609929078	25.177304964539005	25.42109929078014	23.64804964539007
26	25.288888888888888	26.88888888888889	24.933333333333334	22.88888888888889
27	26.052104208416832	25.58450233800935	25.361834780672453	23.00155867290136
28	25.18947837717343	25.813642443156485	25.88051716451181	23.11636201515827
29	24.005364327223962	28.00625838176129	26.26285203397407	21.72552525704068
30	25.823068309070546	25.823068309070546	24.949608062709967	23.404255319148938
31	24.113156713066903	26.47058823529412	26.448136506511	22.968118545127975
32	23.48122866894198	25.961319681456203	26.757679180887372	23.799772468714448
33	24.108352144469524	25.259593679458238	27.516930022573366	23.11512415349887
34	22.972360670593567	28.409605799728137	25.237879474399637	23.38015405527866
35	24.245175936435867	27.264472190692395	24.835414301929625	23.65493757094211
36	22.04778156996587	27.030716723549485	26.075085324232084	24.84641638225256
37	23.70214997378081	25.117986366019927	25.301520713162034	25.87834294703723
38	23.149209259683705	25.12033004813202	26.747650699060276	24.982809993123997
39	22.518382352941178	27.159926470588236	26.217830882352942	24.103860294117645
40	23.216751035434882	26.507132995858264	27.588587206626787	22.687528762080074
41	23.000691403549204	25.720212030421756	26.757317354229087	24.521779211799956
42	25.265834489135457	26.398520573277857	25.104022191400833	23.231622746185852
43	23.824959481361425	27.784209307710118	24.913174345913404	23.47765686501505
44	25.37174721189591	25.882899628252787	25.511152416356875	23.234200743494423
45	25.43635094251804	26.250872701885037	26.46032115429369	21.852455201303233
46	22.75379229871645	27.49124854142357	26.231038506417736	23.52392065344224
47	21.629213483146067	27.715355805243448	25.538389513108616	25.117041198501873
48	23.49906191369606	25.820825515947465	24.202626641651033	26.47748592870544
49	23.091423185673893	27.497643732327994	25.589066918001883	23.82186616399623
50	23.632488752071986	27.27918541321335	26.331991475254558	22.756334359460098
51	22.843488649940262	25.902031063321385	26.714456391875746	24.540023894862603
52	22.472448490656443	25.946334451365598	25.371346430282703	26.20987062769526
53	22.59072338380197	27.349194905070895	24.417207402066808	25.64287430906032
54	23.149492017416545	26.75374939525883	25.592646347363328	24.504112239961295
55	21.041001718634913	26.638841149030203	27.35084704149276	24.969310090842132
56	22.876676308177753	27.26794635813831	27.2942413883776	22.561135945306336
57	24.184210526315788	27.552631578947366	26.94736842105263	21.31578947368421
58	21.806689491703978	29.523307874637872	25.15143534369239	23.518567289965763
59	20.55951438374241	30.509369226708895	26.313011348640803	22.618105040907892
60	21.42668428005284	28.005284015852048	27.767503302509905	22.800528401585204
61	24.424450912939932	26.858957396136546	27.996824556761048	20.719767134162474
62	21.939586645468996	28.37837837837838	28.35188129305776	21.33015368309486
63	23.044285335454788	27.260673561389552	25.96128347918324	23.73375762397242
64	22.352004247411735	27.661268914255377	28.27183435094239	21.714892487390497
65	21.03861517976032	28.868175765645805	26.60452729693742	23.488681757656458
66	22.07099012543368	28.262610088070456	26.74139311449159	22.92500667200427
67	21.915975381321918	29.194541075729198	27.348140219427346	21.54134332352154
68	22.76510067114094	27.060402684563755	26.255033557046982	23.91946308724832
69	22.73462783171521	25.94390507011866	27.885652642934193	23.43581445523193
70	21.842818428184284	26.747967479674795	28.59078590785908	22.818428184281842
71	21.522094926350245	27.577741407528645	28.887070376432078	22.013093289689035
72	22.886257229413385	28.17405673368218	26.30129440925365	22.638391627650787
73	22.209631728045327	28.441926345609065	27.84702549575071	21.5014164305949
74	20.500305064063454	24.405125076266014	30.87248322147651	24.22208663819402
75	21.39495079161318	0.0	43.688489516474114	34.91655969191271
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	3.0
12	4.0
13	5.0
14	7.5
15	9.0
16	9.0
17	9.5
18	13.0
19	16.0
20	14.5
21	19.0
22	30.5
23	31.0
24	26.0
25	39.5
26	59.5
27	74.5
28	88.0
29	96.0
30	104.5
31	112.5
32	123.0
33	126.5
34	128.5
35	145.0
36	153.0
37	153.5
38	158.5
39	165.5
40	165.0
41	162.0
42	178.0
43	184.0
44	175.0
45	166.5
46	162.5
47	165.0
48	166.0
49	174.0
50	181.5
51	177.0
52	174.0
53	172.0
54	177.0
55	169.5
56	150.5
57	136.5
58	128.5
59	140.0
60	147.0
61	135.5
62	128.0
63	117.5
64	99.5
65	96.0
66	88.0
67	68.5
68	50.5
69	41.5
70	36.0
71	29.0
72	25.0
73	24.5
74	25.0
75	22.5
76	17.0
77	13.0
78	13.0
79	20.0
80	30.5
81	42.0
82	46.5
83	105.5
84	157.5
85	83.0
86	10.0
87	2.0
88	1.5
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.131550098662574
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.11069293779056896
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.0580819450697883
33	0.0
34	0.0
35	0.0
36	0.0
37	12.842778793418647
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.05349023803155924
68	0.053662463107056614
69	0.05390835579514825
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	14.0
22-23	21.0
24-25	26.0
26-27	14.0
28-29	21.0
30-31	23.0
32-33	28.0
34-35	19.0
36-37	32.0
38-39	17.0
40-41	20.0
42-43	22.0
44-45	19.0
46-47	21.0
48-49	41.0
50-51	49.0
52-53	40.0
54-55	331.0
56-57	6.0
58-59	12.0
60-61	11.0
62-63	7.0
64-65	20.0
66-67	20.0
68-69	37.0
70-71	59.0
72-73	353.0
74-75	3278.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.77329532997149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.41010053776012	92.28239421179566
2	0.8417114800093525	1.5786011839508878
3	0.2805704933364508	0.7893005919754439
4	0.1402852466682254	0.526200394650296
5	0.02338087444470423	0.10962508221881166
6	0.0	0.0
7	0.02338087444470423	0.15347511510633632
8	0.0701426233341127	0.526200394650296
9	0.0	0.0
>10	0.21042787000233812	4.034203025652269
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGC	38	0.8331506248629686	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	30	0.6577504933128699	No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCC	28	0.6139004604253453	No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC	26	0.5700504275378206	No Hit
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC	17	0.3727252795439596	No Hit
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT	13	0.2850252137689103	No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC	12	0.263100197325148	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	10	0.21925016443762332	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	10	0.21925016443762332	No Hit
CAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	8	0.17540013155009868	No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC	8	0.17540013155009868	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTNTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	8	0.17540013155009868	No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG	7	0.15347511510633632	No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAT	5	0.10962508221881166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
