##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	B-2-R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4616
Sequences flagged as poor quality	0
Sequence length	20-75
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662911611785095	31.0	31.0	34.0	30.0	34.0
2	31.822140381282495	31.0	31.0	34.0	30.0	34.0
3	31.331889081455806	31.0	31.0	34.0	27.0	34.0
4	35.26949740034662	37.0	35.0	37.0	32.0	37.0
5	35.17959272097054	37.0	35.0	37.0	32.0	37.0
6	35.25693240901213	37.0	35.0	37.0	32.0	37.0
7	35.29657712305026	37.0	35.0	37.0	32.0	37.0
8	35.35528596187175	37.0	35.0	37.0	32.0	37.0
9	36.89038128249567	39.0	37.0	39.0	33.0	39.0
10	36.88084922010398	39.0	37.0	39.0	32.0	39.0
11	36.942157712305026	39.0	37.0	39.0	33.0	39.0
12	36.81260831889082	39.0	37.0	39.0	32.0	39.0
13	36.77166377816291	39.0	37.0	39.0	32.0	39.0
14	37.92915944540728	40.0	37.0	41.0	32.0	41.0
15	37.857452339688045	40.0	37.0	41.0	32.0	41.0
16	37.822573656845755	40.0	37.0	41.0	32.0	41.0
17	37.64818024263432	40.0	37.0	41.0	32.0	41.0
18	37.634098786828424	40.0	37.0	41.0	32.0	41.0
19	37.67027729636049	40.0	37.0	41.0	32.0	41.0
20	37.72140381282496	40.0	36.0	41.0	32.0	41.0
21	37.34409534127844	39.0	36.0	41.0	31.0	41.0
22	37.42194275802255	39.0	36.0	41.0	31.0	41.0
23	37.37855437377903	39.0	36.0	41.0	32.0	41.0
24	37.33123234079548	39.0	36.0	41.0	31.0	41.0
25	37.30367631063737	39.0	36.0	41.0	31.0	41.0
26	36.857734204793026	39.0	35.0	41.0	30.0	41.0
27	36.942626527050614	39.0	35.0	41.0	31.0	41.0
28	36.78165938864629	39.0	35.0	41.0	30.0	41.0
29	36.56053321678322	39.0	35.0	41.0	29.0	41.0
30	36.72981842047692	39.0	35.0	41.0	30.0	41.0
31	36.41617357001972	39.0	35.0	41.0	29.0	41.0
32	36.38670761131827	39.0	35.0	40.0	29.0	41.0
33	36.4502307185234	39.0	35.0	40.0	29.0	41.0
34	36.446939674152354	39.0	35.0	40.0	30.0	41.0
35	36.34319134848819	39.0	35.0	40.0	29.0	41.0
36	36.291933701657456	39.0	35.0	40.0	29.0	41.0
37	36.09521700620018	38.0	35.0	40.0	27.0	41.0
38	36.047259818060795	38.0	34.0	40.0	27.0	41.0
39	36.0541981341626	38.0	34.0	40.0	27.0	41.0
40	35.728628673196795	38.0	34.0	40.0	27.0	41.0
41	35.779661016949156	38.0	34.0	40.0	26.0	41.0
42	35.762553001562154	38.0	34.0	40.0	27.0	41.0
43	35.5631567180863	38.0	33.0	40.0	26.0	41.0
44	35.40487914055506	38.0	34.0	40.0	25.0	41.0
45	35.521729390681	38.0	33.0	40.0	26.0	41.0
46	35.49618149146451	38.0	33.0	40.0	26.0	41.0
47	35.212646264626464	38.0	33.0	40.0	25.0	41.0
48	35.315161072313586	38.0	33.0	40.0	25.0	41.0
49	35.143534190927554	38.0	33.0	40.0	26.0	41.0
50	35.24565364642132	38.0	33.0	40.0	26.0	41.0
51	35.3203707890572	38.0	33.0	40.0	26.0	41.0
52	35.50713800135962	38.0	33.0	40.0	27.0	41.0
53	35.29276615104641	38.0	33.0	40.0	26.0	41.0
54	35.29478976234004	38.0	33.0	40.0	26.0	41.0
55	35.60462427745665	38.0	33.0	40.0	27.0	41.0
56	35.97161895360316	38.0	34.0	40.0	29.0	41.0
57	36.01608910891089	38.0	34.0	40.0	29.0	41.0
58	35.97942488844819	38.0	34.0	40.0	29.0	41.0
59	35.867610531544955	38.0	34.0	40.0	29.0	41.0
60	35.83171521035599	37.0	34.0	40.0	29.0	41.0
61	35.682112605879425	37.0	34.0	40.0	28.0	41.0
62	35.5540844366725	37.0	34.0	40.0	28.0	41.0
63	35.39523809523809	37.0	34.0	40.0	28.0	41.0
64	35.25081596786342	37.0	33.0	40.0	28.0	41.0
65	35.14997483643684	36.0	33.0	40.0	28.0	41.0
66	35.17621812673567	36.0	33.0	39.0	28.0	41.0
67	35.13281052365292	36.0	33.0	39.0	28.0	41.0
68	35.182441004821115	36.0	33.0	39.0	29.0	41.0
69	35.04775280898876	36.0	33.0	39.0	29.0	41.0
70	35.03055983564458	36.0	33.0	39.0	29.0	41.0
71	34.94134366925065	35.0	33.0	39.0	29.0	40.0
72	34.820183486238534	35.0	33.0	39.0	29.0	40.0
73	34.79168915272531	35.0	33.0	38.0	29.0	40.0
74	34.848342622469936	35.0	33.0	38.0	29.0	40.0
75	34.37004048582996	35.0	32.0	37.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5792020479520446
1101	2	0.5419833060458004
1101	3	1.8326660607910554
1101	4	0.8763564849502288
1101	5	1.1415858447108462
1101	6	1.0060367583805174
1101	7	-0.05178718850592645
1101	8	1.4213495127557536
1101	9	1.5335907437469913
1101	10	0.4694938741813601
1101	11	0.8967748686498638
1101	12	0.31522216209715737
1101	13	1.723759169071677
1101	14	0.6983798897861391
1101	15	2.2679003288378183
1101	16	2.0904111802549323
1101	17	1.0081597511285025
1101	18	-2.0208259911384943
1101	19	1.1416881151256177
1101	20	2.154747220372222
1101	21	2.6015929961242463
1101	22	2.990198516761005
1101	23	2.959809981684991
1101	24	2.7688184789747226
1101	25	3.1891723438598447
1101	26	3.8457064636752065
1101	27	1.300407231657239
1101	28	0.5527492761867805
1101	29	1.2214960675898254
1101	30	2.7200962232212262
1101	31	3.425396189458695
1101	32	3.2490398490398604
1101	33	0.9780010209697565
1101	34	1.7331645495708017
1101	35	2.7479248182373155
1101	36	3.6178251147001177
1101	37	3.200854643042142
1101	38	3.956891950641946
1101	39	2.6267015970141045
1101	40	2.825479902042389
1101	41	3.3487338471713457
1101	42	3.2187736453361566
1101	43	3.143035841473335
1101	44	3.400118457930951
1101	45	2.1430467680467586
1101	46	2.9878531191031144
1101	47	3.228356076793581
1101	48	1.056088067025577
1101	49	2.065655871905868
1101	50	2.928484131609139
1101	51	2.9144531972656935
1101	52	1.525615067802569
1101	53	1.3245462292337251
1101	54	-0.3702689093314149
1101	55	2.2541459119584175
1101	56	-0.43970473970473734
1101	57	1.914553097365605
1101	58	-0.5431107665482529
1101	59	-2.697353514541021
1101	60	-1.7928607850482834
1101	61	0.4860650345025377
1101	62	1.6661563552188525
1101	63	0.930373330373321
1101	64	1.4846161361786443
1101	65	1.5167540214415283
1101	66	1.7508246961371938
1101	67	-0.43650851463351614
1101	68	0.5651174057424129
1101	69	-4.404718545343538
1101	70	0.16664174945425003
1101	71	1.6348893005142884
1101	72	0.8488244972620009
1101	73	1.8973745004995024
1101	74	1.878745039682535
1101	75	2.501173941798939
1102	1	1.150630619380614
1102	2	1.1134118774743769
1102	3	1.4040946322196248
1102	4	1.3049279135216594
1102	5	1.427300130425131
1102	6	1.5774653298090868
1102	7	1.519641382922643
1102	8	1.4213495127557536
1102	9	0.819305029461276
1102	10	-0.10193469724720927
1102	11	1.4682034400784403
1102	12	2.1723650192400115
1102	13	1.1523305976431004
1102	14	-0.15876296735672213
1102	15	2.2679003288378183
1102	16	0.8046968945406476
1102	17	0.8653026082713566
1102	18	1.5506025802900822
1102	19	0.855973829411333
1102	20	1.440461506086514
1102	21	1.887307281838531
1102	22	1.847341373903859
1102	23	1.3883814102564216
1102	24	1.6259613361175838
1102	25	2.3320294867169835
1102	26	3.274277892246637
1102	27	1.443264374514385
1102	28	2.838463561901065
1102	29	2.9357817818755407
1102	30	3.005810508935511
1102	31	1.425396189458695
1102	32	-3.7509601509601396
1102	33	-0.1648561218873823
1102	34	1.304593120999371
1102	35	0.6050676753801696
1102	36	0.4749679718429718
1102	37	1.91514035732785
1102	38	2.385463379213377
1102	39	1.3409873112998199
1102	40	0.5397656163281042
1102	41	-0.7941232956858002
1102	42	0.790202216764726
1102	43	1.7144644129019113
1102	44	1.400118457930951
1102	45	0.5716181966181892
1102	46	1.1307102619602603
1102	47	-3.6287867803492766
1102	48	0.3418023527398617
1102	49	-0.22005841380841673
1102	50	1.642769845894847
1102	51	1.7715960544085476
1102	52	0.668472210659715
1102	53	1.6102605149480098
1102	54	2.677350138287636
1102	55	1.3017649595774685
1102	56	0.03648573648574427
1102	57	0.12883881165132038
1102	58	1.4330797096422287
1102	59	1.5645512473637453
1102	60	1.5880915959040962
1102	61	1.557493605931107
1102	62	1.5947277837902831
1102	63	2.025611425611416
1102	64	0.8417589933214984
1102	65	1.1596111642986742
1102	66	0.8460627913752887
1102	67	0.8730152948902941
1102	68	0.3984507390757486
1102	69	1.4048052641802684
1102	70	0.7380703208828194
1102	71	1.2301273957523833
1102	72	0.8488244972620009
1102	73	0.4402316433566469
1102	74	0.7716021825396808
1102	75	1.3345072751322746
1103	1	0.2025786713286699
1103	2	0.061463825526324456
1103	3	1.1963024244274223
1103	4	1.2399928485865956
1103	5	0.8169105200355204
1103	6	0.8631796155233715
1103	7	1.078082941364201
1103	8	0.16160925301549156
1103	9	0.014110224266467242
1103	10	0.5474159521034423
1103	11	0.13054110241610317
1103	12	0.08145592833091797
1103	13	-0.4320849867724803
1103	14	0.9840941755004238
1103	15	1.3588094197469118
1103	16	1.2592423490861009
1103	17	1.0211467641155139
1103	18	-0.1377091080216104
1103	19	0.7390907125282169
1103	20	0.7521498177748214
1103	21	0.8743202688515197
1103	22	0.016172542735027662
1103	23	-0.24798222610721155
1103	24	1.0285587387149846
1103	25	1.2281333828208787
1103	26	1.9236285415972887
1103	27	0.6510565823065875
1103	28	0.21508693852444338
1103	29	2.455262301356065
1103	30	1.9149014180264174
1103	31	-0.21096744690493807
1103	32	1.4828060828060998
1103	33	0.6922867352554718
1103	34	0.18771000411625494
1103	35	-0.49882842851593523
1103	36	2.072370569245571
1103	37	2.057997500184996
1103	38	2.5542945480445454
1103	39	-1.6460256757131688
1103	40	0.9683370448995348
1103	41	2.02405852249602
1103	42	0.9330593596218719
1103	43	-0.4413797429422459
1103	44	-0.7167646589521652
1103	45	-0.6101999851999906
1103	46	0.7800609113109118
1103	47	0.968615817053319
1103	48	-0.4633924524549471
1103	49	0.364357170607164
1103	50	1.3310815342065396
1103	51	2.187180469992967
1103	52	1.486654028841535
1103	53	0.9738968785843767
1103	54	2.3440168049543004
1103	55	1.2411588989714062
1103	56	0.8849705849705884
1103	57	1.1742933571058671
1103	58	-0.12752635096383358
1103	59	-0.4506002677877703
1103	60	0.739606747419252
1103	61	1.7847663332038337
1103	62	1.276545965608463
1103	63	-0.4592370592370685
1103	64	-0.5673319157694081
1103	65	-1.2494797447922323
1103	66	-0.3660584207459223
1103	67	-0.4754695535945501
1103	68	0.4742083148333194
1103	69	0.37450223387723724
1103	70	0.46534304815554606
1103	71	-0.48199381636882777
1103	72	0.7579154063529074
1103	73	1.2220498251748282
1103	74	0.11251127344877432
1103	75	-1.6654927248677254
1104	1	0.7934877622377599
1104	2	1.470554734617231
1104	3	2.3326660607910554
1104	4	1.2096898182835645
1104	5	1.9987287018537003
1104	6	1.5298462821900358
1104	7	1.472022335303592
1104	8	1.3737304651367026
1104	9	1.771685981842225
1104	10	-1.4828870781995889
1104	11	-0.12703465515965462
1104	12	0.8390316859066758
1104	13	1.9618544071669106
1104	14	2.150760842167088
1104	15	2.601233662171154
1104	16	1.6380302278739833
1104	17	1.627207370176123
1104	18	2.741078770766272
1104	19	2.7845452579827636
1104	20	1.9642710298960324
1104	21	1.7682596627909106
1104	22	1.39496042152291
1104	23	1.5550480769230859
1104	24	2.149770859927102
1104	25	3.3796485343360345
1104	26	3.3933255112942575
1104	27	3.34802627927629
1104	28	1.7908445142820142
1104	29	3.3643532104469713
1104	30	2.505810508935511
1104	31	2.925396189458695
1104	32	3.725230325230342
1104	33	3.192286735255472
1104	34	1.899831216237466
1104	35	2.319353389665885
1104	36	2.9511584480334534
1104	37	1.557997500184996
1104	38	0.4330824268324278
1104	39	1.6267015970141045
1104	40	1.3016703782328705
1104	41	3.0392100376475355
1104	42	3.433059359621872
1104	43	1.452559650997145
1104	44	2.3286898865023815
1104	45	2.5716181966181892
1104	46	2.5830912143412093
1104	47	3.0140703625078658
1104	48	-1.039150028212518
1104	49	1.470417776667773
1104	50	3.6189603220853286
1104	51	2.247786530599029
1104	52	2.5018055439930507
1104	53	3.776927181614674
1104	54	3.3440168049543004
1104	55	2.468431626244133
1104	56	0.7031524031524086
1104	57	1.6288388116513204
1104	58	2.9330797096422287
1104	59	2.0645512473637453
1104	60	0.4214249292374319
1104	61	0.3908269392644428
1104	62	-1.4052722162097169
1104	63	1.6922780922780873
1104	64	1.8417589933214984
1104	65	3.159611164298674
1104	66	2.6793961247086244
1104	67	-0.7936513717763702
1104	68	1.3984507390757486
1104	69	2.4048052641802684
1104	70	1.2380703208828194
1104	71	1.8967940624190547
1104	72	0.18215783059533663
1104	73	0.873564976689984
1104	74	1.0216021825396808
1104	75	1.8345072751322746
1105	1	0.43634490509490576
1105	2	0.5419833060458004
1105	3	1.8326660607910554
1105	4	-0.6950720864783406
1105	5	0.8558715589965544
1105	6	0.4346081869519409
1105	7	0.9482128114940735
1105	8	0.7070637984700383
1105	9	-0.18069497053872396
1105	10	0.612351017038506
1105	11	0.3253462972212944
1105	12	-0.11334926647427324
1105	13	1.4380448833573922
1105	14	-0.7301915387852915
1105	15	0.9821860431235336
1105	16	1.0904111802549323
1105	17	-0.13469739172864337
1105	18	-3.5922545625670637
1105	19	-0.001169027731521055
1105	20	0.5833186489436528
1105	21	1.3158787104099616
1105	22	0.5616270881895744
1105	23	2.3883814102564216
1105	24	2.649770859927102
1105	25	2.546315201002699
1105	26	3.559992177960922
1105	27	2.0146929459429543
1105	28	2.62417784761535
1105	29	2.697686543780307
1105	30	2.505810508935511
1105	31	2.7587295227920308
1105	32	1.5585636585636706
1105	33	3.0256200685888075
1105	34	3.7331645495708017
1105	35	3.319353389665885
1105	36	3.284491781366782
1105	37	1.557997500184996
1105	38	1.9330824268324278
1105	39	2.4600349303474403
1105	40	3.135003711566199
1105	41	2.5392100376475355
1105	42	3.2663926929552076
1105	43	1.452559650997145
1105	44	3.8286898865023815
1105	45	2.2382848632848535
1105	46	2.0830912143412093
1105	47	2.8474036958412015
1105	48	3.460849971787482
1105	49	3.8037511100011017
1105	50	2.6189603220853286
1105	51	2.9144531972656935
1105	52	2.668472210659715
1105	53	2.276927181614674
1105	54	1.5106834716209647
1105	55	2.135098292910797
1105	56	2.369819069819073
1105	57	1.2955054783179847
1105	58	1.4330797096422287
1105	59	-2.602115419302926
1105	60	-1.4119084040959038
1105	61	-0.4425063940688929
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2305	71	-0.7365392709142782
2305	72	-1.9511755027379962
2305	73	0.6402316433566497
2305	74	0.021602182539680825
2305	75	0.8345072751322746
2306	1	-0.2898455710955723
2306	2	-1.1127785987161012
2306	3	0.7493327274577233
2306	4	0.12635648495022878
2306	5	0.08206203518703603
2306	6	-1.0534870511432928
2306	7	-1.027977664696408
2306	8	0.1237304651367026
2306	9	-0.728314018157775
2306	10	-1.2328870781995889
2306	11	-1.2103679884929903
2306	12	-2.4109683140933242
2306	13	-1.9548122594997537
2306	14	0.3174275088337595
2306	15	0.18456699550448263
2306	16	-0.11196977212601666
2306	17	0.5438740368427872
2306	18	-0.008921229233727956
2306	19	-2.6321214086839007
2306	20	-0.8690623034372962
2306	21	-3.565073670542418
2306	22	-0.3550395784770899
2306	23	-2.2782852564102427
2306	24	-2.850229140072898
2306	25	-1.0370181323306369
2306	26	-2.023341155372414
2306	27	-1.4853070540570457
2306	28	-3.37582215238465
2306	29	-1.9689801228863644
2306	30	-1.6608561577311534
2306	31	-1.7412704772079692
2306	32	-1.774769674769658
2306	33	-1.489531446562708
2306	34	-1.9031990867928315
2306	35	-2.1351920648795684
2306	36	0.7996432965182976
2306	37	-0.12382068163318394
2306	38	1.1906581844081856
2306	39	1.3539743242868312
2306	40	-2.395299318736832
2306	41	0.8422403406778329
2306	42	-0.9760315494690346
2306	43	0.8313475297850275
2306	44	0.737780795593288
2306	45	1.2988909238909159
2306	46	-0.9472118159618219
2306	47	0.7867976352351391
2306	48	0.4456984566359665
2306	49	-0.7265519203019295
2306	50	2.3310815342065396
2306	51	2.5508168336293267
2306	52	1.0321085742960818
2306	53	2.61026051494801
2306	54	2.3440168049543004
2306	55	2.1502498080623127
2306	56	2.430425130425135
2306	57	1.9015660843785938
2306	58	1.599746376308893
2306	59	0.7312179140304096
2306	60	1.1032431110556118
2306	61	1.5120390604765603
2306	62	0.3674550565175565
2306	63	2.0862174862174783
2306	64	2.432668084230592
2306	65	3.023247527935041
2306	66	0.8157597610722576
2306	67	1.342712264587263
2306	68	0.4742083148333194
2306	69	0.7381385975136041
2306	70	0.7380703208828194
2306	71	-2.6638119981870076
2306	72	-0.6966300481925458
2306	73	0.1311407342657418
2306	74	-0.08950892857143344
2306	75	-0.9988260582010611
2307	1	0.19348776223775843
2307	2	0.1705547346172338
2307	3	-4.367333939208944
2307	4	-0.6236435150497712
2307	5	0.2987287018536975
2307	6	-0.036820384476627055
2307	7	1.0053556686369305
2307	8	1.1070637984700369
2307	9	0.9050193151755579
2307	10	0.38377958846707827
2307	11	1.639632011507011
2307	12	0.7723650192400129
2307	13	0.8951877405002477
2307	14	1.1840941755004266
2307	15	0.16790032883781691
2307	16	-0.595303105459351
2307	17	1.3938740368427887
2307	18	1.2077454374329335
2307	19	1.0845452579827608
2307	20	1.6976043632293667
2307	21	0.10159299612424633
2307	22	-0.6383729118104284
2307	23	-0.01161858974357699
2307	24	1.0831041932604393
2307	25	1.1463152010027002
2307	26	0.9599921779609204
2307	27	0.3146929459429515
2307	28	1.3241778476153527
2307	29	1.4643532104469728
2307	30	-0.39418949106448764
2307	31	0.8253961894586936
2307	32	-0.10810300810299367
2307	33	0.09228673525547038
2307	34	-0.5668354504291955
2307	35	0.8193533896658849
2307	36	-2.8821748852998823
2307	37	-3.742002499815001
2307	38	-1.0002509065009093
2307	39	-4.473298402985897
2307	40	-2.431662955100464
2307	41	-0.2941232956858002
2307	42	-0.7669406403781309
2307	43	-4.314107015669521
2307	44	-3.471310113497619
2307	45	-4.2283818033818115
2307	46	-2.283575452325458
2307	47	-2.2859296374921314
2307	48	-2.1724833615458508
2307	49	-0.36291555666556263
2307	50	-1.3143730112480085
2307	51	-4.185546802734308
2307	52	-4.631527789340286
2307	53	-4.189739485051991
2307	54	-3.255983195045701
2307	55	-2.1315683737558686
2307	56	-0.5825618825618761
2307	57	0.057410240222750986
2307	58	-2.1145393379768223
2307	59	-3.1259249431124445
2307	60	-2.364289356476853
2307	61	-2.5139349654974623
2307	62	-3.619557930495432
2307	63	-4.069626669626675
2307	64	-4.801098149535644
2307	65	-2.19753169284418
2307	66	-2.9634610181485215
2307	67	0.13492005679506036
2307	68	0.9936888343138435
2307	69	-0.11900425962924999
2307	70	-2.2619296791171806
2307	71	-0.07939641377142692
2307	72	0.8488244972620009
2307	73	-0.626435023310016
2307	74	0.021602182539680825
2307	75	-3.1654927248677254
2308	1	-0.33151223776224015
2308	2	-0.654445265382769
2308	3	0.9576660607910554
2308	4	0.3138564849502288
2308	5	0.24872870185370033
2308	6	0.050679615523371524
2308	7	0.36785566863692765
2308	8	0.5195637984700383
2308	9	-0.20748068482443927
2308	10	-0.6912204115329246
2308	11	-1.7103679884929903
2308	12	-0.07763498075998854
2308	13	0.04518774050024632
2308	14	-0.9534058244995762
2308	15	-1.9820996711621817
2308	16	-0.6328031054593524
2308	17	0.7313740368427872
2308	18	-1.0922545625670637
2308	19	-0.9654547420172364
2308	20	0.04760436322936812
2308	21	-0.3359070038757537
2308	22	-0.4383729118104256
2308	23	-1.3616185897435784
2308	24	0.9206041932604379
2308	25	-0.5786847989973012
2308	26	-3.752507822039078
2308	27	-2.7353070540570457
2308	28	0.12417784761534989
2308	29	-2.3231467895530287
2308	30	-1.369189491064489
2308	31	-0.07460381054130494
2308	32	-0.23310300810299367
2308	33	1.6297867352554718
2308	34	2.2956645495708017
2308	35	0.5193533896658877
2308	36	0.15115844803344913
2308	37	-1.3420024998150026
2308	38	0.09974909349909211
2308	39	-1.839965069652564
2308	40	-0.4983296217671338
2308	41	-0.32745662901913164
2308	42	-1.7336073070447924
2308	43	-1.1807736823361878
2308	44	0.42868988650238293
2308	45	-1.295048470048478
2308	46	1.483091214341215
2308	47	0.3140703625078629
2308	48	-1.1058166948791879
2308	49	-2.4962488899988955
2308	50	-0.6143730112480057
2308	51	0.8477865305990235
2308	52	-1.331527789340285
2308	53	0.6102605149480098
2308	54	-0.189316528379031
2308	55	-2.1649017070892
2308	56	-1.2301809301809215
2308	57	-1.2378278550153468
2308	58	0.742603519166039
2308	59	1.2312179140304096
2308	60	-0.3642893564768528
2308	61	1.557493605931107
2308	62	0.8090134980759984
2308	63	0.14465904465903634
2308	64	-1.8010981495356475
2308	65	1.0881825928701048
2308	66	0.036538981851478525
2308	67	0.6349200567950604
2308	68	-0.2920254514004412
2308	69	-0.8332899739149653
2308	70	0.23807032088281943
2308	71	1.6348893005142884
2308	72	-0.36546121702371437
2308	73	1.5786931818181884
2308	74	2.021602182539681
2308	75	1.501173941798939
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	8.0
24	16.0
25	44.0
26	69.0
27	78.0
28	146.0
29	123.0
30	156.0
31	210.0
32	229.0
33	262.0
34	289.0
35	315.0
36	415.0
37	557.0
38	734.0
39	872.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.37350964665077	12.096249729026663	19.575113808801213	38.95512681552135
2	23.526863084922013	20.602253032928942	30.589254766031193	25.28162911611785
3	29.116117850953206	23.1369150779896	23.570190641247834	24.176776429809358
4	28.119584055459274	31.499133448873483	18.327556325823224	22.05372616984402
5	28.379549393414212	30.89254766031196	20.86221837088388	19.865684575389945
6	22.98526863084922	35.20363951473137	20.797227036395146	21.013864818024263
7	21.143847487001732	18.089254766031196	39.146447140381284	21.620450606585788
8	23.310225303292896	21.598786828422877	26.126516464471404	28.964471403812826
9	21.837088388214905	23.83015597920277	28.05459272097054	26.278162911611787
10	23.440207972270365	38.128249566724435	20.992201039861353	17.439341421143848
11	27.973997833152765	26.197183098591548	17.594799566630552	28.234019501625134
12	24.241767764298096	24.155112651646444	25.454939341421145	26.148180242634318
13	22.833622183708837	27.946273830155977	27.14471403812825	22.075389948006933
14	23.8136511375948	27.778981581798483	27.042253521126757	21.365113759479957
15	24.956672443674176	25.953206239168107	26.8630849220104	22.227036395147316
16	25.476603119584055	25.086655112651645	25.67157712305026	23.765164644714037
17	25.64991334488735	25.238301559792024	25.909878682842287	23.201906412478337
18	25.34662045060659	27.751299826689774	24.198440207972272	22.703639514731368
19	26.299826689774697	26.819757365684577	23.353552859618716	23.526863084922013
20	25.844887348353552	27.01473136915078	24.198440207972272	22.9419410745234
21	24.507042253521128	26.782231852654387	26.045503791982664	22.66522210184182
22	23.959236773634	27.363399826539464	25.69384215091067	22.98352124891587
23	24.875189928369874	26.95897547210766	25.070544823095293	23.095289776427176
24	25.277113670941098	27.298413388393826	25.255379265377094	22.169093675287982
25	25.45138133565369	26.147487491842508	24.864041766369372	23.537089406134434
26	24.98910675381264	26.143790849673206	25.446623093681918	23.420479302832245
27	25.196335078534034	26.112565445026174	25.610820244328096	23.080279232111693
28	25.174672489082973	26.135371179039304	26.266375545851528	22.4235807860262
29	25.21853146853147	27.90646853146853	25.109265734265733	21.765734265734267
30	26.055567709472765	27.083789105228618	24.633559396193395	22.22708378910523
31	25.2465483234714	26.45189568266491	25.4876177953101	22.813938198553583
32	23.645536301820574	26.54090809388024	26.409300285150252	23.404255319148938
33	24.214458360799824	26.192045704240822	27.092946605141726	22.500549329817623
34	24.328489652135623	25.69352708058124	25.385292822545136	24.592690444738
35	23.681306554844404	27.411167512690355	25.248289560803354	23.659236371661883
36	23.337016574585636	27.005524861878456	25.96685082872928	23.69060773480663
37	23.53852967227635	24.933569530558017	25.752878653675822	25.775022143489814
38	23.008653206123807	26.248058575549145	26.469935655646772	24.273352562680277
39	22.87872056863616	25.8996001776988	27.010217681030653	24.211461572634384
40	22.506678539626	27.13713268032057	26.580587711487087	23.775601068566342
41	24.06333630686887	26.449598572702943	24.754683318465656	24.732381801962532
42	25.039053782637804	25.440749832626647	26.31109127426914	23.209105110466414
43	24.56964006259781	27.833668678739098	24.43550190029063	23.161189358372457
44	24.1047448522829	26.25335720680394	24.48522829006267	25.156669650850493
45	26.478494623655912	25.873655913978492	25.78405017921147	21.863799283154123
46	24.258760107816713	26.41509433962264	25.29200359389039	24.03414195867026
47	22.974797479747973	28.24032403240324	25.36003600360036	23.424842484248426
48	22.099571975670194	25.636404595629646	25.839152962378915	26.42487046632124
49	23.696682464454977	26.811103588354772	25.56984879259761	23.922365154592644
50	23.188078573041317	27.342515240460603	25.694287649582296	23.77511853691578
51	20.890798100836534	26.2717612480217	26.40741578114402	26.430024869997737
52	22.954905959664625	26.73917969635169	25.923408112395197	24.38250623158849
53	23.089171974522294	26.2056414922657	25.34121929026388	25.36396724294813
54	23.171846435100548	26.142595978062154	26.074040219378432	24.611517367458866
55	22.404624277456648	25.75722543352601	25.387283236994218	26.45086705202312
56	23.420533070088844	27.541954590325762	26.283316880552814	22.754195459032577
57	24.207920792079207	26.212871287128714	27.623762376237625	21.955445544554454
58	21.740208230044622	28.011898859692614	27.78879524045612	22.459097669806642
59	22.97565822155986	27.07401887729757	27.148534525583706	22.801788375558868
60	22.13094349016679	27.408513816280806	26.71147622603933	23.749066467513067
61	22.19730941704036	27.179870453413052	27.553562531141008	23.069257598405578
62	22.408193854609042	26.480139895078693	29.053210092430675	22.05845615788159
63	23.007518796992482	25.964912280701753	28.421052631578945	22.606516290726816
64	22.169219181521466	26.261611850364048	27.793120763243785	23.7760482048707
65	22.275358671029448	28.366473697457838	25.698464636294993	23.659702995217717
66	22.973996465539006	28.477657157283513	26.836657409745012	21.711688967432465
67	23.17227422210979	27.852264103212747	27.675183405008852	21.300278269668606
68	23.496574473483886	26.135498604415126	25.856381629028164	24.51154529307282
69	21.27170582226762	26.813074565883554	28.038815117466804	23.876404494382022
70	22.085259373394965	27.503852080123263	27.8376990241397	22.573189522342066
71	22.299741602067183	26.89922480620155	27.906976744186046	22.89405684754522
72	22.096985583224114	26.841415465268675	28.230668414154653	22.830930537352558
73	22.477064220183486	26.71343766864544	27.873718294657312	22.93577981651376
74	21.648577295394546	24.08330888823702	32.15019067175125	22.11792314461719
75	22.793522267206477	0.0	43.92712550607287	33.27935222672065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	0.5
11	0.5
12	2.0
13	4.0
14	5.0
15	3.0
16	1.5
17	5.0
18	8.5
19	12.5
20	15.5
21	19.5
22	24.0
23	26.5
24	36.0
25	50.0
26	66.5
27	77.0
28	79.0
29	96.5
30	109.0
31	106.0
32	110.0
33	124.0
34	149.5
35	159.5
36	156.0
37	156.0
38	159.5
39	163.0
40	157.0
41	152.0
42	169.0
43	174.5
44	167.0
45	168.5
46	169.0
47	162.5
48	155.0
49	160.0
50	171.0
51	181.0
52	178.5
53	168.5
54	163.5
55	157.0
56	147.5
57	130.5
58	121.0
59	134.0
60	127.0
61	116.5
62	113.0
63	94.0
64	81.5
65	72.0
66	68.5
67	59.5
68	46.0
69	44.5
70	33.0
71	23.0
72	23.5
73	24.0
74	18.5
75	18.0
76	20.5
77	18.5
78	20.0
79	23.5
80	32.0
81	46.0
82	51.5
83	123.0
84	192.0
85	102.0
86	13.5
87	8.5
88	4.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06499133448873484
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.02166377816291161
12	0.0
13	0.0
14	0.02166377816291161
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.025163563160543533
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	4.0
22-23	11.0
24-25	11.0
26-27	10.0
28-29	9.0
30-31	12.0
32-33	17.0
34-35	17.0
36-37	18.0
38-39	15.0
40-41	11.0
42-43	13.0
44-45	16.0
46-47	13.0
48-49	10.0
50-51	16.0
52-53	37.0
54-55	324.0
56-57	18.0
58-59	17.0
60-61	14.0
62-63	20.0
64-65	22.0
66-67	20.0
68-69	47.0
70-71	79.0
72-73	406.0
74-75	3409.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.4575389948007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.49327770050996	92.04939341421144
2	0.8113120074177098	1.516464471403813
3	0.25498377375985165	0.7149046793760833
4	0.09272137227630968	0.34662045060658575
5	0.06954102920723226	0.32495667244367415
6	0.02318034306907742	0.12998266897746968
7	0.02318034306907742	0.15164644714038128
8	0.02318034306907742	0.17331022530329288
9	0.02318034306907742	0.1949740034662045
>10	0.16226240148354196	3.162911611785095
>50	0.02318034306907742	1.2348353552859619
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGC	57	1.2348353552859619	No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCC	33	0.7149046793760833	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	29	0.6282495667244368	No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC	27	0.5849220103986136	No Hit
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC	18	0.389948006932409	No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC	13	0.28162911611785096	No Hit
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT	13	0.28162911611785096	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	13	0.28162911611785096	No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG	9	0.1949740034662045	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	8	0.17331022530329288	No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC	7	0.15164644714038128	No Hit
TGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGAC	6	0.12998266897746968	No Hit
CCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	5	0.10831889081455806	No Hit
AAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTT	5	0.10831889081455806	No Hit
TCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCC	5	0.10831889081455806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
