##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	a.chr21.2.fq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4857
Sequences flagged as poor quality	0
Sequence length	50-75
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.56866378422895	31.0	30.0	34.0	25.0	34.0
2	29.841877702285363	31.0	30.0	34.0	25.0	34.0
3	29.873172740374716	31.0	31.0	34.0	25.0	34.0
4	33.265596046942555	37.0	35.0	37.0	26.0	37.0
5	33.21721227094915	37.0	35.0	37.0	26.0	37.0
6	33.0899732345069	37.0	35.0	37.0	25.0	37.0
7	32.95882231830348	37.0	35.0	37.0	25.0	37.0
8	32.95985176034589	37.0	35.0	37.0	25.0	37.0
9	34.366687255507514	38.0	35.0	39.0	25.0	39.0
10	34.355569281449455	38.0	35.0	39.0	25.0	39.0
11	34.45151327980235	38.0	35.0	39.0	26.0	39.0
12	34.17644636606959	38.0	34.0	39.0	25.0	39.0
13	33.92093885114268	38.0	34.0	39.0	19.0	39.0
14	35.12559192917439	39.0	34.0	41.0	24.0	41.0
15	34.71896232242125	39.0	34.0	41.0	18.0	41.0
16	34.95243977764052	39.0	34.0	41.0	23.0	41.0
17	34.89067325509574	39.0	34.0	41.0	23.0	41.0
18	34.792258595841055	39.0	34.0	41.0	19.0	41.0
19	34.8295243977764	39.0	34.0	41.0	19.0	41.0
20	34.64484249536751	39.0	34.0	41.0	18.0	41.0
21	34.35783405394276	39.0	34.0	41.0	18.0	41.0
22	34.308009059089976	39.0	34.0	41.0	18.0	41.0
23	34.18303479514103	39.0	34.0	41.0	17.0	41.0
24	34.2213300391188	39.0	33.0	41.0	18.0	41.0
25	33.88408482602429	38.0	33.0	41.0	16.0	41.0
26	33.86884908379658	38.0	33.0	41.0	16.0	41.0
27	33.76261066501956	38.0	33.0	41.0	15.0	41.0
28	33.71607988470249	38.0	33.0	41.0	15.0	41.0
29	33.48095532221536	38.0	33.0	41.0	10.0	41.0
30	33.333539221741816	38.0	32.0	41.0	10.0	41.0
31	33.3397158739963	38.0	33.0	40.0	10.0	41.0
32	32.97055795758699	38.0	32.0	40.0	8.0	41.0
33	33.04694255713403	38.0	32.0	40.0	9.0	41.0
34	33.04220712373893	38.0	32.0	40.0	9.0	41.0
35	33.034177475808114	38.0	32.0	40.0	9.0	41.0
36	33.00720609429689	38.0	32.0	40.0	8.0	41.0
37	28.237389334980442	37.0	15.0	40.0	2.0	41.0
38	29.596870496191066	36.0	16.0	40.0	5.0	41.0
39	30.98188182005353	36.0	27.0	40.0	2.0	41.0
40	31.558781140621782	37.0	30.0	40.0	2.0	41.0
41	31.884702491249744	38.0	30.0	40.0	2.0	41.0
42	31.906732550957383	38.0	30.0	40.0	2.0	41.0
43	31.820053530986204	38.0	30.0	40.0	2.0	41.0
44	31.761375334568665	38.0	30.0	40.0	2.0	41.0
45	31.784434836318717	38.0	30.0	40.0	2.0	41.0
46	31.63351863290097	37.0	30.0	40.0	2.0	41.0
47	31.378422894791022	37.0	30.0	40.0	2.0	41.0
48	31.406835495161623	37.0	30.0	40.0	2.0	41.0
49	31.544368952028	37.0	30.0	40.0	2.0	41.0
50	31.37677578752316	37.0	30.0	40.0	2.0	41.0
51	30.241032095657648	35.0	28.0	39.0	2.0	40.0
52	30.456261799874135	36.0	28.0	39.0	2.0	40.0
53	31.153345919865743	36.0	30.0	40.0	2.0	41.0
54	31.31613173903923	37.0	30.0	40.0	2.0	41.0
55	31.42752255087057	37.0	30.0	40.0	2.0	41.0
56	34.848980619179464	38.0	34.0	40.0	26.0	41.0
57	34.8139944626227	38.0	34.0	40.0	26.0	41.0
58	34.56506418323685	38.0	34.0	40.0	26.0	41.0
59	34.532343317392396	37.0	34.0	40.0	26.0	41.0
60	34.38434432418827	37.0	33.0	40.0	26.0	41.0
61	34.300025169896806	37.0	33.0	40.0	25.0	41.0
62	34.09539390888497	37.0	33.0	40.0	25.0	41.0
63	33.947394915680846	37.0	33.0	40.0	25.0	41.0
64	33.846715328467155	36.0	33.0	40.0	25.0	41.0
65	33.73470928769192	36.0	33.0	40.0	25.0	41.0
66	33.63402970047823	36.0	33.0	39.0	25.0	41.0
67	33.25119557009816	36.0	32.0	39.0	23.0	41.0
68	33.065190032720864	35.0	32.0	39.0	23.0	41.0
69	33.06317644097659	35.0	32.0	39.0	23.0	41.0
70	32.9992449030959	35.0	33.0	39.0	23.0	41.0
71	32.82607601308835	35.0	33.0	39.0	23.0	41.0
72	32.65190032720866	35.0	33.0	39.0	23.0	40.0
73	32.54870375031462	35.0	33.0	39.0	22.0	40.0
74	32.26302542159577	35.0	32.0	38.0	20.0	40.0
75	31.68663478479738	35.0	32.0	37.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	3.9945443275406483
1101	2	3.591989680730485
1101	3	3.4176741933175734
1101	4	3.6594542593163837
1101	5	3.345525881049788
1101	6	3.182098462199562
1101	7	3.5410766776116063
1101	8	3.666112560505958
1101	9	3.9735851056623233
1101	10	3.0925067477457304
1101	11	4.164260038708569
1101	12	3.518719126393755
1101	13	4.308006153364602
1101	14	5.2021549749950395
1101	15	5.721693019670965
1101	16	5.91392892068442
1101	17	4.887629748383425
1101	18	4.828090060810645
1101	19	5.9562060896619755
1101	20	5.809903573414601
1101	21	6.655614121376992
1101	22	6.7063536477139465
1101	23	6.612624424526999
1101	24	6.736088178229728
1101	25	7.023950511634318
1101	26	6.046427132916108
1101	27	5.972775073510363
1101	28	6.5103816917695525
1101	29	5.479319507766199
1101	30	6.666914413881329
1101	31	6.739023048030404
1101	32	7.193799727301574
1101	33	5.75712255133211
1101	34	4.396025874656381
1101	35	5.91575363804224
1101	36	4.187307069292366
1101	37	11.181964354069134
1101	38	9.893439034753367
1101	39	8.766602387604234
1101	40	8.144808167050812
1101	41	7.854270841495101
1101	42	6.92079569565783
1101	43	7.785019924587928
1101	44	7.779562820417603
1101	45	7.9789844040763285
1101	46	7.355807489079552
1101	47	7.358501875643412
1101	48	7.839488403413036
1101	49	7.551635228197725
1101	50	8.633344962159295
1101	51	8.595934505309504
1101	52	7.79775548525549
1101	53	6.526063259657008
1101	54	7.539644123237871
1101	55	7.554713110963114
1101	56	1.7339533730158792
1101	57	3.5914622414622386
1101	58	3.7373233826358856
1101	59	3.493383988696486
1101	60	5.204226791726789
1101	61	4.478261032948538
1101	62	4.845805525493027
1101	63	5.104943031505535
1101	64	4.901702290764796
1101	65	4.57641444203945
1101	66	4.029173430735938
1101	67	2.799425054112554
1101	68	4.867933802308812
1101	69	5.331300354737856
1101	70	5.246658549783554
1101	71	5.800699705387203
1101	72	5.313667177729684
1101	73	4.523546476671477
1101	74	5.17491432178932
1101	75	5.0689393939393845
1102	1	2.3218170548133763
1102	2	3.101080589821393
1102	3	3.3813105569539417
1102	4	3.6958178956800154
1102	5	2.654616790140693
1102	6	3.036643916745014
1102	7	3.104713041247976
1102	8	0.12065801505141138
1102	9	1.009948742025955
1102	10	1.8561431113820959
1102	11	1.1642600387085693
1102	12	0.7732645809392054
1102	13	-0.32835748299903145
1102	14	1.5294277022677676
1102	15	3.4126021105800604
1102	16	2.2775652843207865
1102	17	2.9785388392925185
1102	18	2.028090060810648
1102	19	4.138024271480155
1102	20	3.39172175523278
1102	21	2.9283413941042653
1102	22	1.3427172840775796
1102	23	2.539897151799728
1102	24	3.5542699964115485
1102	25	1.5875868752706879
1102	26	3.9373362238251985
1102	27	3.881865982601269
1102	28	2.274018055405918
1102	29	2.2247740532207487
1102	30	3.885096232063148
1102	31	4.229932138939496
1102	32	2.7756179091197524
1102	33	3.1207589149684765
1102	34	4.1414804201109305
1102	35	1.6793900016786054
1102	36	0.8600343420196381
1102	37	5.7274188995236806
1102	38	4.547984489298823
1102	39	3.930238751240598
1102	40	3.235717257959905
1102	41	0.49063447785873393
1102	42	2.266250241112374
1102	43	0.16683810640611085
1102	44	-0.03861899776421751
1102	45	1.39716622225815
1102	46	1.8648983981704603
1102	47	-0.31422539708385955
1102	48	0.2940338579584889
1102	49	1.1334534100159033
1102	50	0.7787995076138436
1102	51	1.3959345053095014
1102	52	2.9977554852554924
1102	53	0.8351541687479127
1102	54	0.30328048687423603
1102	55	1.5001676564176591
1102	56	0.08950892857143344
1102	57	0.6803511303511272
1102	58	0.24843449374699844
1102	59	0.44893954425204186
1102	60	0.8708934583934536
1102	61	-0.3661834114959035
1102	62	-1.2875278078403056
1102	63	-0.33950141293890823
1102	64	-2.253853264790763
1102	65	-1.4235855579605499
1102	66	-0.5708265692640637
1102	67	-0.1116860569985576
1102	68	-0.04317730880229931
1102	69	-0.8909218674843657
1102	70	-2.6422303391053354
1102	71	-0.4881891835016816
1102	72	-1.2418883778258731
1102	73	0.05687981000481557
1102	74	0.3971365440115413
1102	75	0.22449494949494309
1103	1	2.4695443275406497
1103	2	3.4419896807304866
1103	3	2.642674193317575
1103	4	1.1844542593163823
1103	5	2.3705258810497867
1103	6	1.8320984621995606
1103	7	2.991076677611609
1103	8	3.041112560505958
1103	9	1.4985851056623218
1103	10	1.867506747745729
1103	11	2.5392600387085693
1103	12	0.9437191263937521
1103	13	1.8080061533646017
1103	14	3.927154974995041
1103	15	4.571693019670967
1103	16	3.1639289206844197
1103	17	3.012629748383425
1103	18	3.278090060810648
1103	19	3.3312060896619755
1103	20	5.0849035734146
1103	21	3.280614121376992
1103	22	5.3313536477139465
1103	23	5.312624424527002
1103	24	3.4860881782297284
1103	25	4.848950511634321
1103	26	4.721427132916105
1103	27	4.347775073510363
1103	28	4.660381691769551
1103	29	3.304319507766202
1103	30	5.066914413881328
1103	31	5.514023048030403
1103	32	6.2187997273015725
1103	33	5.25712255133211
1103	34	5.221025874656384
1103	35	3.1907536380422385
1103	36	4.587307069292365
1103	37	10.181964354069134
1103	38	7.21843903475337
1103	39	5.191602387604231
1103	40	6.644808167050812
1103	41	2.854270841495101
1103	42	4.095795695657827
1103	43	2.235019924587931
1103	44	2.7795628204176026
1103	45	3.45398440407633
1103	46	2.080807489079554
1103	47	0.3335018756434138
1103	48	3.7144884034130357
1103	49	5.826635228197723
1103	50	5.733344962159297
1103	51	5.395934505309501
1103	52	3.2477554852554924
1103	53	4.801063259657006
1103	54	1.6896441232378692
1103	55	1.2047131109631124
1103	56	-0.8410466269841237
1103	57	-0.3335377585377586
1103	58	-3.237676617364116
1103	59	-2.2316160113035153
1103	60	-1.4207732082732107
1103	61	-0.17173896705146063
1103	62	-3.07919447450697
1103	63	-1.3950569684944654
1103	64	-1.0732977092352058
1103	65	-1.4235855579605499
1103	66	-1.0708265692640637
1103	67	-0.37557494588744333
1103	68	0.19293380230881496
1103	69	0.5813003547378557
1103	70	-0.25334145021644616
1103	71	-1.2243002946127959
1103	72	-0.436332822270316
1103	73	-1.4014535233285201
1103	74	-0.8250856782106801
1103	75	-0.20606060606061405
1104	1	2.5036352366315597
1104	2	1.6465351352759399
1104	3	1.472219647863028
1104	4	2.8776360774982024
1104	5	2.7455258810497867
1104	6	3.2184620985631938
1104	7	2.741076677611609
1104	8	2.029748924142325
1104	9	2.1008578329350485
1104	10	2.856143111382096
1104	11	2.527896402344936
1104	12	2.500537308211932
1104	13	3.398915244273695
1104	14	4.074882247722314
1104	15	5.41260211058006
1104	16	3.823019829775326
1104	17	2.7967206574743315
1104	18	4.118999151719741
1104	19	3.683478816934702
1104	20	4.482630846141873
1104	21	5.201068666831539
1104	22	4.97908092044122
1104	23	2.9944426063451814
1104	24	4.190633632775182
1104	25	4.587586875270688
1104	26	4.573699860188832
1104	27	2.9727750735103626
1104	28	4.728563509951371
1104	29	4.133864962311655
1104	30	3.885096232063148
1104	31	5.411750320757676
1104	32	5.502890636392479
1104	33	5.666213460423016
1104	34	6.050571329201837
1104	35	4.952117274405872
1104	36	2.4963979783832713
1104	37	9.363782535887314
1104	38	8.638893580207917
1104	39	7.748420569422411
1104	40	4.053899076141718
1104	41	5.3997253869496475
1104	42	5.629886604748734
1104	43	5.439565379133384
1104	44	4.688653729508509
1104	45	3.9426207677126968
1104	46	5.773989307261374
1104	47	5.86759278473432
1104	48	3.748579312503942
1104	49	5.133453410015903
1104	50	6.233344962159297
1104	51	4.486843596218595
1104	52	5.270482757982766
1104	53	3.653335986929733
1104	54	4.939644123237869
1104	55	5.500167656417659
1104	56	2.867286706349212
1104	57	1.9025733525733557
1104	58	2.9151011604136627
1104	59	2.115606210918706
1104	60	2.2042267917267893
1104	61	1.5227054773929822
1104	62	2.934694414381916
1104	63	1.9938319203944204
1104	64	2.0794800685425727
1104	65	2.3541922198172287
1104	66	2.4291734307359363
1104	67	2.666091720779221
1104	68	3.4012671356421507
1104	69	2.99796702140452
1104	70	3.0244363275613324
1104	71	4.067366372053868
1104	72	3.6470005110630197
1104	73	2.6124353655603656
1104	74	3.5082476551226556
1104	75	4.33560606060605
1105	1	2.5112109942073175
1105	2	2.1919896807304866
1105	3	0.017674193317574804
1105	4	1.392787592649718
1105	5	2.5788592143831224
1105	6	-0.41790153780043937
1105	7	1.6577433442782734
1105	8	1.4994458938392938
1105	9	1.2902517723289861
1105	10	1.242506747745729
1105	11	1.8309267053752336
1105	12	2.568719126393752
1105	13	1.3913394866979374
1105	14	2.302154974995041
1105	15	4.321693019670967
1105	16	3.830595587351084
1105	17	2.9709630817167607
1105	18	3.028090060810648
1105	19	2.289539422995311
1105	20	1.8765702400812643
1105	21	2.4889474547103276
1105	22	2.289686981047282
1105	23	3.3126244245270016
1105	24	2.6527548448963927
1105	25	2.723950511634321
1105	26	2.5130937995827693
1105	27	4.806108406843698
1105	28	2.3270483584362154
1105	29	1.3459861744328663
1105	30	0.4002477472146637
1105	31	-3.1109769519695973
1105	32	0.5104663939682368
1105	33	1.8404558846654453
1105	34	2.262692541323048
1105	35	1.1490869713755743
1105	36	1.753973735959029
1105	37	6.848631020735798
1105	38	3.1767723680867057
1105	39	-0.18339761239576902
1105	40	-1.5218584996158562
1105	41	-2.562395825171567
1105	42	-2.029204304342173
1105	43	-1.4316467420787369
1105	44	-1.3037705129157295
1105	45	-0.2543489292570058
1105	46	-4.460859177587114
1105	47	-2.3748314576899183
1105	48	-2.4938449299202965
1105	49	-2.0483647718022766
1105	50	-2.5999883711740353
1105	51	-1.1040654946904986
1105	52	-1.5022445147445076
1105	53	-1.407270073676326
1105	54	0.27297745657120487
1105	55	0.0380464442964481
1105	56	0.6248624639249698
1105	57	0.6096440596440615
1105	58	-2.5899493446368425
1105	59	-3.5611614658489685
1105	60	-3.6139550264550273
1105	61	-1.9217389670514606
1105	62	-2.4996490199615167
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2306	21	-0.8443858786230081
2306	22	-1.6269796856193892
2306	23	-0.10404224213966273
2306	24	-1.680578488436936
2306	25	-2.692716155032347
2306	26	-3.570239533750563
2306	27	-0.5272249264896374
2306	28	-1.9229516415637846
2306	29	-2.487347158900466
2306	30	-1.933085586118672
2306	31	-1.6109769519695973
2306	32	-0.5728669393650918
2306	33	-1.7428774486678904
2306	34	-0.9039741253436162
2306	35	-0.7675796952910972
2306	36	0.25397373595902906
2306	37	5.2652976874024695
2306	38	2.7601057014200343
2306	39	1.2332690542708953
2306	40	1.2281415003841474
2306	41	0.9376041748284365
2306	42	-2.529204304342173
2306	43	0.6516865912545953
2306	44	-0.8871038462490652
2306	45	-3.0043489292570023
2306	46	0.12247415574621812
2306	47	0.9585018756434138
2306	48	2.3394884034130357
2306	49	1.1183018948643877
2306	50	0.2333449621592969
2306	51	0.14593450530950136
2306	52	-2.7522445147445076
2306	53	-2.407270073676326
2306	54	-1.2270225434287987
2306	55	0.2880464442964481
2306	56	-2.7660466269841244
2306	57	1.5914622414622386
2306	58	3.137323382635884
2306	59	0.6933839886964819
2306	60	0.3042267917267907
2306	61	-0.9217389670514606
2306	62	0.44580552549302865
2306	63	2.1049430315055346
2306	64	3.501702290764797
2306	65	-1.4235855579605499
2306	66	-1.1708265692640651
2306	67	-1.7005749458874426
2306	68	-3.8320661976911836
2306	69	-3.2686996452621457
2306	70	-3.753341450216446
2306	71	-2.099300294612796
2306	72	0.11366717772968116
2306	73	0.22354647667147987
2306	74	-3.32508567821068
2306	75	-3.6310606060606148
2307	1	-5.2387890057926825
2307	2	-3.3080103192695134
2307	3	-5.482325806682425
2307	4	-2.77387907401695
2307	5	-0.9211407856168776
2307	6	-2.7512348711337715
2307	7	-4.092256655721723
2307	8	-6.25055410616071
2307	9	-6.543081561004346
2307	10	-6.507493252254271
2307	11	-6.335739961291431
2307	12	-6.847947540272916
2307	13	-10.691993846635398
2307	14	-8.947845025004959
2307	15	-8.844973646995701
2307	16	-10.919404412648912
2307	17	-8.862370251616575
2307	18	-12.38857660585602
2307	19	-12.127127243671357
2307	20	-14.123429759918732
2307	21	-11.42771921195634
2307	22	-11.460313018952721
2307	23	-10.437375575472998
2307	24	-10.263911821770272
2307	25	-10.276049488365679
2307	26	-10.820239533750563
2307	27	-11.277224926489637
2307	28	-12.172951641563781
2307	29	-11.987347158900466
2307	30	-11.34975225278534
2307	31	-10.527643618636265
2307	32	-10.322866939365095
2307	33	-9.49287744866789
2307	34	-9.903974125343616
2307	35	-10.017579695291094
2307	36	-10.329359597374303
2307	37	-5.734702312597534
2307	38	-6.90656096524663
2307	39	-8.100064279062437
2307	40	-8.68852516628252
2307	41	-10.729062491838231
2307	42	-10.612537637675505
2307	43	-12.014980075412069
2307	44	-10.137103846249065
2307	45	-11.004348929257002
2307	46	-11.294192510920446
2307	47	-10.791498124356586
2307	48	-10.493844929920296
2307	49	-10.381698105135609
2307	50	-10.933321704507371
2307	51	-10.770732161357166
2307	52	-10.252244514744508
2307	53	-11.323936740342994
2307	54	-10.477022543428799
2307	55	-10.62862022237022
2307	56	1.2006200396825406
2307	57	1.7914622414622414
2307	58	1.3039900493025485
2307	59	-1.2732826779701796
2307	60	1.3708934583934536
2307	61	0.9115943662818751
2307	62	0.5458055254930301
2307	63	-2.7283903018277975
2307	64	0.8017022907647942
2307	65	0.9097477753727858
2307	66	1.5958400974026006
2307	67	0.9994250541125567
2307	68	2.567933802308815
2307	69	2.99796702140452
2307	70	-0.08667478354978186
2307	71	1.7340330387205398
2307	72	1.6470005110630197
2307	73	0.7235464766714799
2307	74	2.841580988455984
2307	75	2.8356060606060502
2308	1	1.483433216429539
2308	2	1.6364341251749295
2308	3	2.3510075266509105
2308	4	3.1705653704274965
2308	5	0.18997032549422954
2308	6	1.5820984621995606
2308	7	0.9632988998338305
2308	8	1.5550014493948439
2308	9	2.7069184389956575
2308	10	2.7147289699679504
2308	11	2.7198155942641264
2308	12	1.8742746819493092
2308	13	1.3080061533646017
2308	14	2.1354883083283767
2308	15	2.6550263530043026
2308	16	2.469484476239977
2308	17	0.2209630817167607
2308	18	2.139201171921762
2308	19	1.6228727563286398
2308	20	3.4321257956368214
2308	21	1.3222807880436562
2308	22	3.039686981047282
2308	23	1.8126244245270016
2308	24	3.2916437337852855
2308	25	2.223950511634321
2308	26	2.5130937995827693
2308	27	3.750552851288141
2308	28	2.799270580658437
2308	29	4.012652841099538
2308	30	0.7335810805479923
2308	31	2.0279119369192884
2308	32	2.0382441717460154
2308	33	3.868233662443224
2308	34	1.262692541323048
2308	35	3.53797586026446
2308	36	4.809529291514586
2308	37	5.848631020735798
2308	38	3.537883479197813
2308	39	1.8999357209375667
2308	40	3.255919278161926
2308	41	3.409826397050658
2308	42	5.498573473435606
2308	43	5.540575480143488
2308	44	5.112896153750938
2308	45	5.023428848520773
2308	46	5.8446963779684395
2308	47	6.736279653421192
2308	48	5.839488403413036
2308	49	1.284968561531059
2308	50	0.011122739937075465
2308	51	3.17371228308728
2308	52	2.4421999296999353
2308	53	3.4816188152125633
2308	54	3.4951996787934263
2308	55	4.843601999851998
2308	56	1.6450644841269906
2308	57	1.0136844636844629
2308	58	-0.7515655062530016
2308	59	0.44893954425204186
2308	60	0.9820045695045678
2308	61	1.856038810726318
2308	62	-0.0653055856180842
2308	63	2.882720809283313
2308	64	2.746146735209237
2308	65	-0.09025222462721416
2308	66	0.5402845418470505
2308	67	0.9994250541125567
2308	68	-1.932066197691185
2308	69	1.7757447991822986
2308	70	1.4688807720057753
2308	71	-1.154855850168353
2308	72	-1.1307772667147589
2308	73	0.05687981000481557
2308	74	-0.9361967893217908
2308	75	-1.4421717171717248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	133.0
3	12.0
4	24.0
5	18.0
6	18.0
7	21.0
8	26.0
9	27.0
10	19.0
11	30.0
12	31.0
13	29.0
14	36.0
15	31.0
16	29.0
17	31.0
18	29.0
19	28.0
20	38.0
21	49.0
22	45.0
23	25.0
24	47.0
25	54.0
26	57.0
27	65.0
28	97.0
29	108.0
30	110.0
31	139.0
32	191.0
33	214.0
34	295.0
35	307.0
36	429.0
37	577.0
38	658.0
39	693.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.18985776128633	11.729540300968873	18.181818181818183	40.898783755926615
2	24.397776405188388	20.032942145357215	29.915585752522134	25.65369569693226
3	29.359687049619104	23.388923203623634	22.503603047148445	24.747786699608813
4	29.40086473131563	29.812641548280833	18.077002264772492	22.70949145563105
5	29.730286184887788	30.183240683549517	20.547663166563723	19.538809964998972
6	22.81243565987235	34.69219682931851	20.094708667901998	22.400658842907145
7	22.297714638665845	17.088737904056	39.0982087708462	21.515338686431953
8	23.59481161210624	21.721227094914557	25.838995264566606	28.8449660284126
9	23.12126827259625	22.606547251389745	28.330245007206095	25.941939468807906
10	23.63598929380276	36.91579164093062	21.04179534692197	18.40642371834466
11	29.133209800288245	25.36545192505662	18.591723285979	26.90961498867614
12	24.66543133621577	22.318303479514103	27.671402100061766	25.344863084208356
13	24.809553222153593	26.580193535103973	26.786081943586577	21.824171299155857
14	25.015441630636193	27.0331480337657	26.00370599135269	21.94770434424542
15	25.838995264566606	25.633106856084005	25.1183858348775	23.409512044471896
16	26.168416718138772	24.006588429071442	26.00370599135269	23.8212888614371
17	27.074325715462223	26.35371628577311	23.471278567016675	23.10067943174799
18	26.10665019559399	26.333127444924852	23.86246654313362	23.69775581634754
19	26.044883673049206	25.777228742021823	24.068354951616225	24.109532633312742
20	26.765493102738315	27.136092238007	24.068354951616225	22.03005970763846
21	24.52130945027795	28.02141239448219	24.212476837554046	23.244801317685816
22	25.30368540251184	25.591929174387484	25.036030471484455	24.068354951616225
23	26.45666049001441	27.074325715462223	23.388923203623634	23.080090590899733
24	26.23018324068355	27.794935145151328	24.356598723491867	21.618282890673253
25	26.360263808738665	25.185490519373456	24.85572959604287	23.598516075845012
26	25.612518015235743	26.786081943586577	24.315421041795346	23.285978999382333
27	26.90961498867614	25.4272184476014	24.747786699608813	22.91537986411365
28	25.797817582870085	25.591929174387484	25.344863084208356	23.265390158534075
29	25.159563516574018	27.177269919703523	25.818406423718343	21.84476014000412
30	26.991970352069178	25.571340333539222	24.233065678402305	23.203623635989292
31	25.344863084208356	25.797817582870085	25.674284537780522	23.183034795141033
32	24.08805031446541	25.429769392033542	26.47798742138365	24.0041928721174
33	24.60366481367099	25.138974675725756	27.197858760551778	23.059501750051474
34	23.8212888614371	27.95964587193741	24.60366481367099	23.6154004529545
35	24.768375540457072	26.786081943586577	24.150710315009267	24.294832200947088
36	22.585958410541487	26.49783817171093	25.633106856084005	25.283096561663577
37	24.45067788686302	24.31042543244507	25.1986909770921	26.04020570359981
38	23.75952233889232	24.72719785876055	26.744904261890056	24.768375540457072
39	22.99773522750669	26.41548280831789	25.838995264566606	24.747786699608813
40	24.43895408688491	25.880172946263123	26.539015853407456	23.141857113444512
41	23.73893349804406	24.994852789787934	26.291949763228327	24.974263948939672
42	26.539015853407456	25.5301626518427	24.50072060942969	23.430100885320158
43	24.912497426394893	26.971381511220923	24.068354951616225	24.047766110767963
44	26.518427012559194	25.20074119827054	24.62425365451925	23.656578134651017
45	26.66254889849701	25.880172946263123	25.30368540251184	22.15359275272802
46	24.315421041795346	26.55960469425571	25.20074119827054	23.9242330656784
47	22.87420218241713	27.218447601400044	24.50072060942969	25.40662960675314
48	24.459542927733168	25.056619312332717	23.6154004529545	26.868437306979615
49	24.047766110767963	27.0331480337657	24.397776405188388	24.52130945027795
50	25.40662960675314	26.66254889849701	24.768375540457072	23.162445954292775
51	23.18019718900776	25.445773022865538	25.949234319278375	25.424795468848334
52	23.494860499265783	25.15208726662471	24.50178309209146	26.85126914201804
53	23.222152297042165	26.515628277742813	23.809523809523807	26.452695615691212
54	23.725613593455	26.09607719739878	24.963289280469898	25.21501992867632
55	21.73274596182085	26.767358925949235	25.76043633312356	25.739458779106357
56	22.92977598791845	27.233828341303802	27.258998238107225	22.577397432670526
57	24.137931034482758	27.510697206141455	27.233828341303802	21.117543418071985
58	22.073999496602063	29.222250188774225	25.2705763906368	23.43317392398691
59	20.9161842436446	30.405235338535114	25.975333501132646	22.703246916687643
60	21.62094135414045	28.265794110244148	27.41001761892776	22.703246916687643
61	24.817518248175183	26.881449786055878	27.586206896551722	20.714825069217216
62	22.401208155046564	28.265794110244148	28.064434935816763	21.268562798892525
63	23.231814749559526	27.435187515731187	25.67329473949157	23.659702995217717
64	22.52705763906368	27.837905864585956	27.913415554996224	21.721620941354143
65	21.193053108482253	28.94538132393657	26.025673294739494	23.83589227284168
66	22.50188774226026	28.03926503901334	26.62975081802165	22.829096400704756
67	22.23342576254096	29.241240231913284	26.947315351651124	21.57801865389463
68	23.040080665490294	27.17418704310562	25.888580791530124	23.89715149987396
69	22.53592135114696	26.29190824300479	27.451474665994454	23.720695739853795
70	22.149509187012335	26.931789579662723	28.089604832620186	22.829096400704756
71	22.023659702995218	27.73722627737226	28.29096400704757	21.94815001258495
72	23.03045557513214	28.517493078278378	25.82431412031211	22.62773722627737
73	22.77875660709791	29.675308331235843	26.47873143720111	21.06720362446514
74	21.193053108482253	29.297759879184493	27.46035741253461	22.04882959979864
75	20.76516486282406	27.96375534860307	28.240624213440725	23.03045557513214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.5
9	2.0
10	1.5
11	1.5
12	1.5
13	0.5
14	0.5
15	1.5
16	2.0
17	4.5
18	9.5
19	12.0
20	12.0
21	16.5
22	27.5
23	28.0
24	22.0
25	37.0
26	60.5
27	79.0
28	88.5
29	92.5
30	103.0
31	118.0
32	130.0
33	124.0
34	121.5
35	141.0
36	154.0
37	154.5
38	154.5
39	156.5
40	159.0
41	164.0
42	186.5
43	189.0
44	174.0
45	166.5
46	173.0
47	177.0
48	167.0
49	173.0
50	185.5
51	187.0
52	180.0
53	175.5
54	179.5
55	167.0
56	148.0
57	141.0
58	140.5
59	151.0
60	155.0
61	141.0
62	129.5
63	115.0
64	98.0
65	96.0
66	81.0
67	55.0
68	41.5
69	47.0
70	41.0
71	27.0
72	24.5
73	26.0
74	26.0
75	20.5
76	17.0
77	15.5
78	13.0
79	13.0
80	14.0
81	21.5
82	28.0
83	101.0
84	175.0
85	100.5
86	16.0
87	6.0
88	4.0
89	2.0
90	1.0
91	0.0
92	1.0
93	2.0
94	1.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.12353304508956146
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.10294420424130121
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.7912291537986413
33	0.0
34	0.0
35	0.0
36	0.0
37	11.92093885114268
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.15101938082053865
68	0.15101938082053865
69	0.15101938082053865
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50	90.0
51	0.0
52	0.0
53	0.0
54	0.0
55	794.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	3973.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.63804817788758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.52682497801231	92.25859584105415
2	0.7695690413368513	1.4412188593782171
3	0.2418645558487247	0.6794317479925881
4	0.13192612137203166	0.49413218035824585
5	0.04397537379067722	0.20588840848260243
6	0.0	0.0
7	0.0	0.0
8	0.06596306068601583	0.49413218035824585
9	0.0	0.0
>10	0.21987686895338612	4.426600782375952
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGC	47	0.9676755198682314	No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCC	31	0.6382540662960675	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	30	0.6176652254478072	No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC	30	0.6176652254478072	No Hit
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC	19	0.3911879761169446	No Hit
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	13	0.2676549310273832	No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC	12	0.24706609017912293	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAA	12	0.24706609017912293	No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC	11	0.22647724933086266	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAG	10	0.20588840848260243	No Hit
CAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	8	0.16471072678608192	No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG	8	0.16471072678608192	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTNTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAAT	8	0.16471072678608192	No Hit
CTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAAC	5	0.10294420424130121	No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAT	5	0.10294420424130121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.020588840848260244	0.0	0.0	0.0	0.0
26	0.020588840848260244	0.0	0.0	0.0	0.0
27	0.020588840848260244	0.0	0.0	0.0	0.0
28	0.020588840848260244	0.0	0.0	0.0	0.0
29	0.020588840848260244	0.0	0.0	0.0	0.0
30	0.020588840848260244	0.0	0.0	0.0	0.0
31	0.04117768169652049	0.0	0.0	0.0	0.0
32	0.04117768169652049	0.0	0.0	0.0	0.0
33	0.04117768169652049	0.0	0.0	0.0	0.0
34	0.06176652254478073	0.0	0.0	0.0	0.0
35	0.06176652254478073	0.0	0.0	0.0	0.0
36	0.06176652254478073	0.0	0.0	0.0	0.0
37	0.06176652254478073	0.0	0.0	0.0	0.0
38	0.06176652254478073	0.0	0.0	0.0	0.0
39	0.08235536339304098	0.0	0.0	0.0	0.0
40	0.08235536339304098	0.0	0.0	0.0	0.0
41	0.08235536339304098	0.0	0.0	0.0	0.0
42	0.08235536339304098	0.0	0.0	0.0	0.0
43	0.08235536339304098	0.0	0.0	0.0	0.0
44	0.08235536339304098	0.0	0.0	0.0	0.0
45	0.08235536339304098	0.0	0.0	0.0	0.0
46	0.08235536339304098	0.0	0.0	0.0	0.0
47	0.08235536339304098	0.0	0.0	0.0	0.0
48	0.08235536339304098	0.0	0.0	0.0	0.0
49	0.10294420424130121	0.0	0.0	0.0	0.0
50	0.10294420424130121	0.0	0.0	0.0	0.0
51	0.10294420424130121	0.0	0.0	0.0	0.0
52	0.10294420424130121	0.0	0.0	0.0	0.0
53	0.12353304508956146	0.0	0.0	0.0	0.0
54	0.12353304508956146	0.0	0.0	0.0	0.0
55	0.12353304508956146	0.0	0.0	0.0	0.0
56	0.12353304508956146	0.0	0.0	0.0	0.0
57	0.12353304508956146	0.0	0.0	0.0	0.0
58	0.12353304508956146	0.0	0.0	0.0	0.0
59	0.12353304508956146	0.0	0.0	0.0	0.0
60	0.12353304508956146	0.0	0.0	0.0	0.0
61	0.12353304508956146	0.0	0.0	0.0	0.0
62	0.12353304508956146	0.0	0.0	0.0	0.0
63	0.12353304508956146	0.0	0.0	0.0	0.0
64	0.12353304508956146	0.0	0.0	0.0	0.0
>>END_MODULE
