diff --git a/README.md b/README.md index c6d48a8074a0ef740c54525d8706dd400604c2c4..ed199ef33a9f477b63eea3e4f1ed945a87756efb 100644 --- a/README.md +++ b/README.md @@ -16,8 +16,9 @@ A[1. For each brain region separately, merge BAM files with SAMtools] --> B[2. F D --> E[5. Combine all peaks with rbind in R. These peaks are the Regions of Open Chromatin or ROCs]; E --> F[6. Keep only intergenic ROCs or iROCs]; F --> G[7. Quantify transcription within iROCs]; - G --> H[8. Identify all genes within 1MB up and downstream from TAPE center]; - H --> I[9. Correlate eRNA and mRNA transcriptional abundance]; + G --> H[8. Identify iROCs with bidirectional transcription. These are Transcriptionally Active Putative Enhancers or TAPEs] + H --> I[9. Identify all genes within 1MB up and downstream from TAPE center]; + I --> J[10. Correlate eRNA and mRNA levels for predicted enhancer-gene pairs]; style A fill:#98EFFF,stroke:#000,stroke-width:2px; style B fill:#98EFFF,stroke:#000,stroke-width:2px; style C fill:#98EFFF,stroke:#000,stroke-width:2px; @@ -26,7 +27,8 @@ A[1. For each brain region separately, merge BAM files with SAMtools] --> B[2. F style F fill:#98EFFF,stroke:#000,stroke-width:2px; style G fill:#98EFFF,stroke:#000,stroke-width:2px; style H fill:#98EFFF,stroke:#000,stroke-width:2px; - style I fill:#98EFFF,stroke:#000,stroke-width:2px; + style I fill:#98EFFF,stroke:#000,stroke-width:2px; + style J fill:#98EFFF,stroke:#000,stroke-width:2px; ``` ## **NGS Experimental Details**