diff --git a/README.md b/README.md index cb65988bf4a0e82e87ad2ce5fd690d990caa74c1..03ee07076abbca6c06f50fbd967abe6fff6b7d2a 100644 --- a/README.md +++ b/README.md @@ -10,7 +10,7 @@ The diagram below outlines a general workflow for the identifcation of enhancers ```mermaid graph TD; -A[1. For each brain region separately, merge BAM files with SAMtools] --> B[2. For each brain region separately, call peaks with MACS2]; +A[1. For each brain region separately, merge BAM files with SAMtools] --> B[2. For each brain region separately, Call peaks with MACS2]; B --> C[3. For each brain region separately, merge peaks within 1kb using bedtools]; C --> D[4. For each brain region separately, remove any peaks smaller than 146 bp or the legnth of DNA wrapped around a nucleosome]; D --> E[5. Combine all peaks with rbind in R. These peaks are the Regions of Open Chromatin or ROCs];